2 research outputs found
Acid-Resistant Mesoporous Metal–Organic Frameworks as Carriers for Targeted Hypoglycemic Peptide Delivery: Peptide Encapsulation, Release, and Bioactivity
Oral administration of bioactive peptides with α-glucosidase
inhibitory activities is a promising strategy for diabetes mellitus.
The wheat germ peptide Leu–Asp–Leu–Gln–Arg
(LDLQR) has been previously proven to inhibit the activity of α-glucosidase
efficiently. However, it is still difficult to transport the peptide
to the intestine completely due to the harsh condition of the stomach.
Herein, an acid-resistant zirconium-based metal–organic framework,
NU-1000, was used to immobilize LDLQR with a high encapsulation capacity
(92.72%) and encapsulation efficiency (44.08%) in only 10 min. The in vitro release results showed that the acid-stable NU-1000
not only effectively protected LDLQR from degradation in the presence
of stomach acid and pepsin effectively but also ensured the release
of encapsulated LDLQR under simulated intestinal conditions. Furthermore,
LDLQR@NU-1000 could slow down the elevated blood sugar caused by maltose
in mice and the area under blood sugar curve decreased by almost 20%
when compared with the control group. The inflammatory factor (IL-1β,
IL-6) in vivo and cell growth in vitro were almost the same between NU-1000 treatment and normal control
groups. This study indicates NU-1000 is a promising vehicle for targeted
peptide-based bioactive delivery to the small intestine
Molecularly Imprinted Electrochemical Sensor Based on α‑Cyclodextrin Inclusion Complex and MXene Modification for Highly Sensitive and Selective Detection of Alkylresorcinols in Whole Wheat Foods
Authenticating whole wheat foods poses a significant
challenge
for both the grain industry and consumers. Alkylresorcinols (ARs),
serving as biomarkers of whole wheat, play a crucial role in assessing
the authenticity of whole wheat foods. Herein, we introduce a novel
molecularly imprinted electrochemical sensor with modifications involving
a molecularly imprinted polymer (MIP) and MXene nanosheets, enabling
highly sensitive and selective detection of ARs. Notably, we specifically
chose 5-heneicosylresorcinol (AR21), the predominant homologue in
whole wheat, as the template molecule. α-Cyclodextrin and acrylamide
served as dual functional monomers, establishing a robust multiple
interaction between the MIP and AR21. As a result, the sensor exhibited
a wide linear range of 0.005 to 100 μg·mL–1 and a low detection limit of 2.52 ng·mL–1, demonstrating exceptional selectivity and stability. When applied
to commercial whole wheat foods, the assay achieved satisfactory recoveries
and accuracy, strongly validating the practicality and effectiveness
of this analytical technique