33 research outputs found
Ectopic expression of RMRP promoted lung adenocarcinoma cell proliferation, colony formation and invasion.
<p>(A) The expression of RMRP was measured in the H1299 cell after treated with RMRP vector. (B) Ectopic expression of RMRP promoted H1299 cell proliferation. (C) Overexpression of RMRP enhanced the cyclin D1 expression in the H1299 cell. (D) Ectopic expression of RMRP promoted ki-67 expression in the H1299 cell. (E) Overexpression of RMRP promoted the H1299 cell colony formation. (F) Overexpression of RMRP enhanced the H1299 cellinvasion. *p<0.05, **p<0.01 and ***p<0.001.</p
miR-206 expression was downregulated in the lung adenocarcinoma tissues.
<p>(A) The miR-206 expression was measured in lung adenocarcinoma cell lines (A549, SPC-A1, H1299 and H23) and the bronchial epithelial cell line using qRT-PCR. (B) The miR-206 expression was detected in lung adenocarcinoma tissues and the matched adjacent normal tissues by using qRT-PCR. (C) miR-206 expression was downregulated in 21 cases (21/35; 60%) compared to the adjacent normal tissues. (D) The expression of RMRP was negative correlated with the expression of miR-206 in lung adenocarcinoma tissues.</p
RMRP suppressed expression of miR-206 and increased the expression of KRAS, FMNL2 and SOX9.
<p>(A) Overexpression of RMRP inhibited theexpression of miR-206 in the H1299 cell. (B) Ectopic expression ofRMRP promoted the KRAS mRNA expression in the H1299 cell. (C) The protein expression of KRAS was determined using western blot. (D) Ectopic expression ofRMRP promoted the FMNL2 mRNA expression in the H1299 cell. (E) The protein expression ofFMNL2 was determined using western blot. (F) Ectopic expression ofRMRP promoted the SOX9 mRNA expression in the H1299 cell. (G) The protein expression ofSOX9 was determined using western blot.</p
The expression of RMRP was upregulated in the lung adenocarcinoma tissues.
<p>(A) RMRP expression was measured in the lung adenocarcinoma tissues and the matched adjacent normal tissues using qRT-PCR. (B) The RMRP was upregulated in 25 cases (25/35; 71.4%) compared to the adjacent normal tissues. (C) The RMRP expression was upregulated in lung adenocarcinoma cell lines (A549, SPC-A1, H1299 and H23) compared to the bronchial epithelial cell line (16HBE).</p
RMRP exhibited an oncogenic activity through targeting miR-206.
<p>(A) miR-206 expression was upregulated in H1299 cell after treated with the miR-206 mimic. (B) miR-206 expression was decreased in the RMRP-induced H1299 cell after treated with RMRP vector. (C) Restoration of miR-206 suppressed cell proliferation in the RMRP-induced H1299 cell after treated with miR-206 mimic. (D) Restoration of miR-206 inhibited the cell invasion in the RMRP-induced H1299 cell after treated with miR-206 mimic.*p<0.05, **p<0.01 and ***p<0.001.</p
CuWO<sub>4</sub> Nanoflake Array-Based Single-Junction and Heterojunction Photoanodes for Photoelectrochemical Water Oxidation
Over recent years, tremendous efforts
have been invested in the search and development of active and durable
semiconductor materials for photoelectrochemical (PEC) water splitting,
particularly for photoanodes operating under a highly oxidizing environment.
CuWO<sub>4</sub> is an emerging candidate with suitable band gap and
high chemical stability. Nevertheless, its overall solar-to-electricity
remains low because of the inefficient charge separation process.
In this work, we demonstrate that this problem can be partly alleviated
through designing three-dimensional hierarchical nanostructures. CuWO<sub>4</sub> nanoflake arrays on conducting glass are prepared from the
chemical conversion of WO<sub>3</sub> templates. Resulting electrode
materials possess large surface areas, abundant porosity and small
thickness. Under illumination, our CuWO<sub>4</sub> nanoflake array
photoanodes exhibit an anodic current density of ∼0.4 mA/cm<sup>2</sup> at the thermodynamic potential of water splitting in pH 9.5
potassium borate buffer î—¸ the largest value among all available
CuWO<sub>4</sub>-based photoanodes. In addition, we demonstrate that
their performance can be further boosted to >2 mA/cm<sup>2</sup> by coupling with a solution-cast BiVO<sub>4</sub> film in a heterojunction
configuration. Our study unveils the great potential of nanostructured
CuWO<sub>4</sub> as the photoanode material for PEC water oxidation
Efficient Photoelectrochemical Hydrogen Evolution on Silicon Photocathodes Interfaced with Nanostructured NiP<sub>2</sub> Cocatalyst Films
Increasing attention
has now been focused on the photoelectrochemical (PEC) hydrogen evolution
as a promising route to transforming solar energy into chemical fuels.
Silicon is one of the most studied PEC electrode materials, but its
performance is still limited by its inherent PEC instability and electrochemical
inertness toward water splitting. To achieve significant PEC activities,
silicon-based photoelectrodes usually have to be coupled with proper
cocatalysts, and thus, the formed semiconductor–cocatalyst
interface presents a critical structural parameter in the rational
design of efficient PEC devices. In this study, we directly grow nanostructured
pyrite-phase nickel phosphide (NiP<sub>2</sub>) cocatalyst films on
textured pn<sup>+</sup>-Si photocathodes via on-surface reaction at
high temperatures. The areal loading of the cocatalyst film can be
tailored to achieve an optimal balance between its optical transparency
and electrocatalytic activity. As a result, our pn<sup>+</sup>-Si/Ti/NiP<sub>2</sub> photocathodes demonstrate a great PEC onset potential of
0.41 V versus reversible hydrogen electrode (RHE), a decent photocurrent
density of ∼12 mA/cm<sup>2</sup> at the thermodynamic potential
of hydrogen evolution, and an impressive operation durability for
at least 6 h in 0.5 M H<sub>2</sub>SO<sub>4</sub>. Comparable PEC
performance is also observed in 1 M potassium borate buffer (pH =
9.5) using this device
Controllably Interfacing with Ferroelectric Layer: A Strategy for Enhancing Water Oxidation on Silicon by Surface Polarization
Silicon (Si) is an important material
in photoelectrochemical (PEC) water splitting because of its good
light-harvesting capability as well as excellent charge-transport
properties. However, the shallow valence band edge of Si hinders its
PEC performance for water oxidation. Generally, thanks to their deep
valence band edge, metal oxides are incorporated with Si to improve
the performance, but they also decrease the transportation of carriers
in the electrode. Here, we integrated a ferroelectric polyÂ(vinylidene
fluoride–trifluoroethylene) [PÂ(VDF–TrFE)] layer with
Si to increase the photovoltage as well as the saturated current density.
Because of the prominent ferroelectric property from PÂ(VDF–TrFE),
the Schottky barrier between Si and the electrolyte can be facially
tuned by manipulating the poling direction of the ferroelectric domains.
The photovoltage is improved from 460 to 540 mV with a forward-poled
PÂ(VDF–TrFE) layer, while the current density increased from
5.8 to 12.4 mA/cm<sup>2</sup> at 1.23 V bias versus reversible hydrogen
electrode
Liquid Phase Exfoliated MoS<sub>2</sub> Nanosheets Percolated with Carbon Nanotubes for High Volumetric/Areal Capacity Sodium-Ion Batteries
The search for high-capacity,
low-cost electrode materials for
sodium-ion batteries is a significant challenge in energy research.
Among the many potential candidates, layered compounds such as MoS<sub>2</sub> have attracted increasing attention. However, such materials
have not yet fulfilled their true potential. Here, we show that networks
of liquid phase exfoliated MoS<sub>2</sub> nanosheets, reinforced
with 20 wt % single-wall carbon nanotubes (SWNTs), can be formed into
sodium-ion battery electrodes with large gravimetric, volumetric,
and areal capacity. The MoS<sub>2</sub>/SWNT composite films are highly
porous, electrically conductive, and mechanically robust due to its
percolating carbon nanotube network. When directly employed as the
working electrode, they exhibit a specific capacity of >400 mAh/g
and volumetric capacity of ∼650 mAh/cm<sup>3</sup>. Their mechanical
stability allows them to be processed into free-standing films with
tunable thickness up to ∼100 μm, corresponding to an
areal loading of 15 mg/cm<sup>2</sup>. Their high electrical conductivity
allows the high volumetric capacity to be retained, even at high thickness,
resulting in state-of-the-art areal capacities of >4.0 mAh/cm<sup>2</sup>. Such values are competitive with their lithium-ion counterparts
Covalent Hybrid of Spinel Manganese–Cobalt Oxide and Graphene as Advanced Oxygen Reduction Electrocatalysts
Through direct nanoparticle nucleation and growth on
nitrogen doped,
reduced graphene oxide sheets and cation substitution of spinel Co<sub>3</sub>O<sub>4</sub> nanoparticles, a manganese–cobalt spinel
MnCo<sub>2</sub>O<sub>4</sub>/graphene hybrid was developed as a highly
efficient electrocatalyst for oxygen reduction reaction (ORR) in alkaline
conditions. Electrochemical and X-ray near-edge structure (XANES)
investigations revealed that the nucleation and growth method for
forming inorganic–nanocarbon hybrids results in covalent coupling
between spinel oxide nanoparticles and N-doped reduced graphene oxide
(N-rmGO) sheets. Carbon K-edge and nitrogen K-edge XANES showed strongly
perturbed C–O and C–N bonding in the N-rmGO sheet, suggesting
the formation of C–O–metal and C–N–metal
bonds between N-doped graphene oxide and spinel oxide nanoparticles.
Co L-edge and Mn L-edge XANES suggested substitution of Co<sup>3+</sup> sites by Mn<sup>3+</sup>, which increased the activity of the catalytic
sites in the hybrid materials, further boosting the ORR activity compared
with the pure cobalt oxide hybrid. The covalently bonded hybrid afforded
much greater activity and durability than the physical mixture of
nanoparticles and carbon materials including N-rmGO. At the same mass
loading, the MnCo<sub>2</sub>O<sub>4</sub>/N-graphene hybrid can outperform
Pt/C in ORR current density at medium overpotentials with stability
superior to Pt/C in alkaline solutions