Abnormal interruption of water flow from an artesian well prior to 2008 Wenchuan earthquake

Water flow from an artesian well stopped on December 17, 2007 but recovered when the Wenchuan Ms 8.0 earthquake occurred on May 12, 2008. This well is located 90 km south of the epicenter in an extensional tectonic setting, where similar changes of water level and resistivity were observed at two other nearby sites. Our investigation suggests that this phenomenon was not caused by environmental disturbances, such as drought or borehole-drilling activity, but might be a precursor to the earthquake

The Advanced Synthesis of MOFs-Based Materials in Photocatalytic HER in Recent Three Years

Since the advent of metal–organic frameworks (MOFs), researchers have paid extensive attention to MOFs due to their determined structural composition, controllable pore size, and diverse physical and chemical properties. Photocatalysis, as a significant application of MOFs catalysts, has developed rapidly in recent years and become a research hotspot continuously. Various methods and approaches to construct and modify MOFs and their derivatives can not only affect the structure and morphology, but also largely determine their properties. Herein, we summarize the advanced synthesis of MOFs-based materials in the field of the photocatalytic decomposition of water to produce hydrogen in the recent three years. The main contents include the overview of the novel synthesis strategies in four aspects: internal modification and structure optimization of MOFs materials, MOFs/semiconductor composites, MOFs/COFs-based hybrids, and MOFs-derived materials. In addition, the problems and challenges faced in this direction and the future development goals were also discussed. We hope this review will help deepen the reader’s understanding and promote continued high-quality development in this field

A novel super-enhancer-related gene signature predicts prognosis and immune microenvironment for breast cancer

Abstract Background This study targeted at developing a robust, prognostic signature based on super-enhancer-related genes (SERGs) to reveal survival prognosis and immune microenvironment of breast cancer. Methods RNA-sequencing data of breast cancer were retrieved from The Cancer Genome Atlas (TCGA), 1069 patients of which were randomly assigned into training or testing set in 1:1 ratio. SERGs were downloaded from Super-Enhancer Database (SEdb). After which, a SERGs signature was established based on the training set, with its prognostic value further validated in the testing set. Subsequently, we identified the potential function enrichment and tumor immune infiltration of the model. Moreover, in vitro experiments were completed to further explore the biological functions of ZIC2 gene (one of the risk genes in the prognostic model) in breast cancer. Results A risk score system of prognostic value was constructed with 6 SERGs (ZIC2, NFE2, FOXJ1, KLF15, POU3F2 and SPIB) to find patients in high-risk group with significantly worse prognosis in both training and testing sets. In addition, a multivariate regression was established via integrating the 6 genes with age and N stage, indicating well performance by calibration, time-dependent receiver operating characteristic (ROC) analysis and decision curve analysis (DCA). Further analysis demonstrated that tumor-associated pathological processes and pathways were significantly enriched in the high-risk group. In general, the novel SERGs signature could be applied to screen breast cancer with immunosuppressive microenvironment for the risk score was negatively correlated with ESTIMATE score, tumor-infiltration lymphocytes (such as CD4 + and CD8 + T cell), immune checkpoints and chemotactic factors. Furthermore, down-regulation of ZIC2 gene expression inhibited the cell viability, cellular migration and cell cycle of breast cancer cells. Conclusions The novel SERGs signature could predict the prognosis of breast cancer; and SERGs might serve as potential therapeutic targets for breast cancer

Upregulation of KHDC1L promotes the proliferation and inhibits apoptosis in head and neck squamous cell carcinoma

Head and neck squamous cell carcinoma (HNSCC) remains a dreadful malignancy bearing poor clinical efficacy, with emerging evidences indicating RNA-binding proteins’ (RBPs’) relevance to the evolution of the disease. Categorized as RBPs, the K-homology domain-containing 1 (KHDC1) family is proved to be closely related to cell survival and death. As a novel KHDC1 member, only one study is currently available in osteoarthritis synovial cells to unveil KHDC1L’s function of promoting proliferation. Nevertheless, to the best of our knowledge, the role of KHDC1L in human tumour is yet to be fully explored. On the basis of The Cancer Genome Atlas (TCGA) database and cell lines comparison with normal counterparts in this study, we first discovered KHDC1L to be overexpressed in HNSCC. According to bioinformatics analysis, apoptosis and P53 pathways were remarkably enriched in the KHDC1L low-expression group in TCGA database. Moreover, in vitro experiments were applied to verify that upregulation of KHDC1L could promote the proliferation and inhibit apoptosis in HNSCC cells CAL27. Transcriptome sequencing ascertained downstream differentially expressed genes to be significantly enriched in PI3K-AKT pathways. Furthermore, as validated by western blot, we found an elevated expression level of pAKT/AKT and Bcl-2, constant expression level of BAX, together with decreased activity of Caspase-3 and PARP-1 in the KHDC1L-upregulated group. In conclusion, our study pioneeringly elaborated that KHDC1L could promote proliferation and inhibit apoptosis in HNSCC cell CAL27 via AKT and Bcl-2 pathways, representing a crucial step for seeking a new diagnostic and therapeutic target in HNSCC

Geological characteristics and potential evaluation of typical interlayer shale oil in the Ordos Basin: A case study of the Chang 7 Member of Well Ning228

The Chang 7 Member of the Ordos Basin has favorable shale oil resource potentiality. The exploration and development of sandstone interlayer shale oil in the Chang 7 Member has made substantial breakthroughs. The exploration and development potential of shale oil is huge, and will be a significant oil and gas alternative resource in the Changqing Oilfield in the future, but its related geological fundamental research is relatively weak. Based on the observation, description and systematic sampling of the centimeter-level core of the full core section of Chang 7 Member in Well Ning228, a series of experimental test analyses and identification outcomes indicated that Chang 7 Member primarily developed four rock types: Black shale, dark mudstone, siltstone and tuff. The overall conditions of source rocks in the Chang 7 member are superior, the organic carbon content of shale is high, and the hydrocarbon generation potential is large. The organic matter types are mainly type Ⅰ and type Ⅱ, which are in the low mature-mature stage, and the black shale has higher organic matter abundance. The main reservoir spaces of the Chang 7 Member are primary pores and secondary pores. Mud shale primarily develops organic pores and inorganic pores. Fine sandstone mainly develops residual intergranular pores, intragranular pores and intergranular pores. Tuff mainly develops intergranular pores and mineral intragranular pores. The overall oil-bearing property of the Chang 7 Member is good, but the mobility difference is large. The fluid mobility in sandstone and tuff is good, and the mobility inshale is poor. In short, fine anatomical analysis of typical interlayer shale oil provides favorable guidance for promoting the large-scale development of shale oil in the Ordos Basin

The Analysis of Lithosphere–Atmosphere–Ionosphere Coupling Associated with the 2022 Luding Ms6.8 Earthquake

Taking the Luding Ms6.8 earthquake (EQ) on 5 September 2022 as a case study, we investigated the potential seismic anomalies of the ionosphere, infrared radiation, atmospheric electrostatic field (AEF), and hot spring ions in the seismogenic region. Firstly, we analyzed the multi-parameter anomalies in the ionosphere around the epicenter and found synchronous anomalous disturbances in the ground parameters, namely the global ionospheric map (GIM), GPS, TEC, and satellite parameters, such as the He+ and O+ densities on 26 August under relatively quiet solar–geomagnetic conditions (F10.7 −30 nT; |AE| < 500 nT). Next, both the anomaly analysis of the infrared radiation and AEF, and the survey results of the Luding EQ scientific expedition on the hot spring ions showed pre-seismic anomalous variations at different time periods in the seismogenic region. The characteristics of Earth’s multi-sphere coupling anomalies in temporal evolution and spatial distribution were obvious, which validated the Lithosphere–Atmosphere–Ionosphere Coupling (LAIC) mechanism. Finally, combining the analysis results and the LAIC mechanism, we suggested that the multi-sphere coupling anomalies were more likely associated with the Luding Ms6.8 EQ, and that the differential motion and the regional crustal stress accumulation between the Chuandian block and the Bayan Har block might have led to this EQ. Furthermore, remote sensing and ground-based monitoring technologies can play an important role in corroborating and compensating each other, while further study of the multi-sphere coupling mechanism will provide a clearer understanding of the seismogenic process for major EQs

Spring floods and their major influential factors in the upper reaches of Jinsha River basin during 2001–2020

Study region: The upper reaches of Jinsha River basin (UJSB) in the northeastern Tibetan Plateau. Study focus: Spring floods in the UJSB have posed increasingly severe challenges to reservoirs operation and water resources management due to increased climatic variability under global warming. In this study, spring floods in the region were investigated for 2001–2020 based on station data and multisource remote sensing products. We seek to understand: 1) the characteristics including the peaks, the frequency, and duration of spring floods, 2) how snowfall and rainfall affect spring floods. New hydrological insights for the region: Late May has seen most of the highest spring flood discharge, while some springs have experienced multiple peaks. Extreme spring floods were identified in the years 2012, 2013, 2019, and 2020, with the highest peak discharge (1365.83 m3/s) and longest flood duration (47 days) in 2019. Spring snowmelt dominated the extreme spring floods in most years while the floods in 2012 and 2020 were results of combined snowmelt and rainfall. We defined Snow Water Volume (SWV) as an indicator of the precondition for high spring flood. The key regions contributing to spring floods in the UJSB were concentrated around the Tongtianhe sub-basin and the northern parts of Batang–Zhimenda sub-basin where the SWV was large. The enhanced Westerly jets in winters brought about large snowfall and extended snow cover in the UJSB which can be released as floods triggered by rapid increase in air temperature in the coming spring

Cigarette smoke triggers calcium overload in mouse hippocampal neurons via the ΔFOSB-CACNA2D1 axis to impair cognitive performance

A growing body of evidence shows that cigarette smoking impairs cognitive performance. The 'Calcium Hypothesis' theory of neuronopathies reveals a critical role of aberrant calcium signaling in compromised cognitive functions. However, the underlying implications of abnormalities in calcium signaling in the neurotoxicity induced by cigarette smoke (CS) have not yet been identified. CACNA2D1, an important auxiliary subunit involved in the composition of voltage-gated calcium channels (VGCCs), was reported to affect the calcium signaling in neurons by facilitating VGCCs-mediated Ca2+ influx. ΔFOSB, an alternatively-spliced product of the Fosb gene, is an activity-dependent transcription factor induced robustly in the brain in response to environmental stimuli such as CS. Interestingly, our preliminary bioinformatics analysis revealed a significant co-expression between ΔFOSB and CACNA2D1 in brain tissues of patients with neurodegenerative diseases characterized by progressive cognitive decline. Therefore, we hypothesized that the activation of the ΔFOSB-CACNA2D1 axis in response to CS exposure might cause dysregulation of calcium homeostasis in hippocampal neurons via VGCCs-mediated Ca2+ influx, thereby contributing to cognitive deficits. To this end, the present study established a CS-induced mouse model of hippocampus-dependent cognitive impairment, in which the activation of the ΔFOSB-CACNA2D1 axis accompanied by severe calcium overload was observed in the mouse hippocampal tissues. More importantly, ΔFOSB knockdown-/overexpression-mediated inactivation/activation of the ΔFOSB-CACNA2D1 axis interdicted/mimicked CS-induced dysregulation of calcium homeostasis followed by severe cellular damage in HT22 mouse hippocampal neurons. Mechanistically speaking, a further ChIP-qPCR assay confirmed the physical interaction between transcription factor ΔFOSB and the Cacna2d1 gene promoter, suggesting a direct transcriptional regulation of the Cacna2d1 gene by ΔFOSB. Overall, our current work aims to deliver a unique insight into the neurotoxic mechanisms induced by CS to explore potential targets for intervention