942

After quanlity control, this experimental population consists of 942 young Simmental bulls born between 2008 and 2011, which are originated from Ulgai, Xilingol league, Inner Mongolia of China. After weaning, the cattle were moved to Beijing Jinweifuren cattle farm and were fattened under the same feeding and management environment. Each individual was timely observed for growth and development traits until slaughtered from 16 to 18 months old. During the period of slaughter, carcass traits and meat quality traits were measured according to Institutional Meat Purchase Specifications for fresh beef guidelines

Socio-demographic characteristics of children aged 6–17 years in nine provinces in China: China Health and Nutrition Survey 2004–2009 (cross-sectional analysis).

<p>Socio-demographic characteristics of children aged 6–17 years in nine provinces in China: China Health and Nutrition Survey 2004–2009 (cross-sectional analysis).</p

LANA interacts with the IL-22R1 promoter in vivo.

<p>(<b>A</b>) Schematic diagram showing the locations of the two pairs of primers used in the ChIP assay. (<b>B</b>) Formaldehyde cross-linked chromatin was prepared from 293T cells that were transfected with pFLAG, pFLAG-LANA, pFLAG-LANA_1–939 or pFLAG-LANA_933–1162, and immunoprecipitated with anti-FLAG antibody. PCR was performed with primer pair 1 to amplify a 197 bp DNA fragment containing LBS or with primer pair 2 to amplify a 283 bp DNA fragment, approximately 2 kb upstream of LBS-like sequence in IL-22R1 promoter. A sample representative of 5% the total input chromatin was included in the PCR analysis. (<b>C</b>) The expression levels of LANA and its truncated mutants were detected in cell lysates by western blotting using anti-FLAG antibody and anti-actin served as a loading control.</p

Funnel plot of the association of COX-2 positivity with clinical parameters.

<p>Funnel plot of the association of COX-2 positivity with clinical parameters.</p

Quantitative Assessment of the Association of COX-2 (Cyclooxygenase-2) Immunoexpression with Prognosis in Human Osteosarcoma: A Meta-Analysis

<div><p>Background</p><p>Numerous studies examining the relationship between Cyclooxygenase-2 (COX-2) immunoexpression and clinical outcome in osteosarcoma patients have yielded inconclusive results. </p> <p>Methods</p><p>We accordingly conducted a meta-analysis of 9 studies (442 patients) that evaluated the correlation between COX-2 immunoexpression and clinical prognosis (death). Pooled odds ratios (OR) and risk ratios (RR) with 95% confidence intervals (95% CI) were calculated using the random-effects or fixed-effects model. </p> <p>Results</p><p>Meta–analysis showed no significant association between COX-2 positivity and age, gender, tumor location, histology, stage, metastasis or 90% necrosis. Conversely, COX-2 immunoexpression was associated with overall survival rate (RR=2.12; 95% CI: 1.10–3.74; P=0.009) and disease-free survival rate (RR=1.63; 95% CI: 1.17–2.28; P=0.004) at 2 years. Sensitivity analysis performed by omitting low quality studies showed that the pooled results were stable. </p> <p>Conclusions</p><p>COX-2 positivity was associated with a lower 2-year overall survival rate and disease-free survival rate. COX-2 expression change is an independent prognostic factor in patients with osteosarcoma.</p> </div

Funnel plot of the association between COX-2 positivity and overall survival rate at 2 years by Sensitivity analysis.

<p>Funnel plot of the association between COX-2 positivity and overall survival rate at 2 years by Sensitivity analysis.</p

Linear and logistic regression analysis for cross-sectional and longitudinal associations between Chinese children’s Western fast food consumption and their BMI z-scores and weight status: China Health and Nutrition Survey 2004–2009.

<p>Linear and logistic regression analysis for cross-sectional and longitudinal associations between Chinese children’s Western fast food consumption and their BMI z-scores and weight status: China Health and Nutrition Survey 2004–2009.</p

Map of the China Health and Nutrition Survey (CHNS) coverage 2004–2009.

<p>Map of the China Health and Nutrition Survey (CHNS) coverage 2004–2009.</p

The LBS-like sequence is required for LANA to down-regulate IL-22R1 expression.

<p>(<b>A</b>) Schematic of pIL22R1 (−2139 to +39), pIL22R1ΔLBS-like and pIL22R1mLBS-like DNA sequences. (<b>B</b>) Increasing amounts of pcDNA-LANA expressing full-length LANA were co-transfected with either pIL22R1 (−2139 to +39) or its mutants (pIL22R1ΔLBS-like and pIL22R1mLBS-like) into 293T cells. At 48 h post-transfection, cells were harvested and assayed for luciferase activity. (<b>C</b>) Increasing amounts of pcDNA-LANA-C-expressing carboxyl-terminal domain (amino acids 951–1162) of LANA were co-transfected with either pIL22R1 (−2139 to +39) or its mutants (pIL22R1ΔLBS-like and pIL22R1mLBS-like) into 293T cells. At 48 h post-transfection, cells were harvested and assayed for luciferase activity. (<b>D</b>) Increasing amounts of pcDNA-LANA expressing full-length LANA were co-transfected with either pIL22R1 (−2139 to +39) or its mutants (pIL22R1ΔLBS-like and pIL-22R1mLBS-like) into HUVEC cells. At 48 h post-transfection, cells were harvested and assayed for luciferase activity.</p

The process flow diagram describes how we filtered the data we retrieved.

<p>The process flow diagram describes how we filtered the data we retrieved.</p