43 research outputs found
Key fault propagation path identification of CNC machine tools based on maximum occurrence probability
In order to revise the deviation caused by ignoring the dynamic character of fault propagation in traditional fault propagation path identification methods, a method based on the maximum occurrence probability is proposed to identify the key fault propagation path. Occurrence probability of fault propagation path is defined by dynamic importance, dynamic fault propagation probability and fault rate. Taking the fault information of CNC machine tools whichsubject to Weibull distribution as an example, this method has been proven to be reasonable through comparative analysis. Result shows that the key fault propagation path of CNC machine tools is not unique, but changes with time. Before 1000 hours, key fault propagation path is electrical component (E) to mechanical component (M); after 1000 hours, key fault propagation path is auxiliary component (A) to mechanical component (M). This change should be taken into account when developing maintenance strategies and conducting reliability analysis
Molecular and Physiological Characterization of Two Novel Multirepeat β-Thymosins from Silkworm, Bombyx mori.
β-thymosin plays important roles in the development of the lymphatic system and the central nervous system in vertebrates. However, its role and function in invertebrates remain much less explored. Here, we firstly isolated a gene encoding β-thymosin in silkworm (Bombyx mori L.). Interestingly, this gene encodes two polypeptides, named as BmTHY1 and BmTHY2, via two different modes of RNA splicing. The recombinant proteins fused with an N-term GST tag were over-expressed in Escherichia coli (E. coli) and further purified to near homogenity to prepare mouse antibodies. The Western blot analysis showed that these proteins were expressed in various tissues and organs, as well as in different developmental stages. Amazingly, the expression of BmTHY2 was hugely increased during the pupae stage, indicating a specialized role in this period. The expression of these proteins was gradually decreased in BmN cells infected by BmNPV, suggesting they may play different roles in the virus infection. In addition, both BmTHY1 and BmTHY2 can interact with 14-3-3 of silkworm and Ubiquitin of BmNPV as shown by GST pull down and Co-IP assays, consistent with their roles in the regulation of the development of nervous system
Additional file 8 of Identification of crucial genes and metabolites regulating the eggshell brownness in chicken
Additional file 8: Table S7. Differential metabolites between LBS and DBS groups at 6 h following oviposition in uterine fluid
Additional file 3 of Identification of crucial genes and metabolites regulating the eggshell brownness in chicken
Additional file 3: Table S2. Differentially upregulated genes in DBS group at 22 h compared with 4 h following oviposition
Additional file 9 of Identification of crucial genes and metabolites regulating the eggshell brownness in chicken
Additional file 9: Table S8. Differential metabolites between LBS and DBS groups at 22 h following oviposition in uterine fluid
Additional file 1 of Identification of crucial genes and metabolites regulating the eggshell brownness in chicken
Additional file 1: Fig. S1. Eggs in shell glands of WS, LBS, and DBS groups at 16 hours and 22 hours following oviposition
Additional file 7 of Identification of crucial genes and metabolites regulating the eggshell brownness in chicken
Additional file 7: Table S6. Differential metabolites between LBS and DBS groups at 22 h following oviposition in shell glands
The expression pattern of BmTHYs in BmN cells infected with BmNPV.
<p>The BmN cells were infected with BmNPV and samples were collected at different hours to examine the expression of BmTHYs. The mass of each lane’s total protein were 30 μg, and the sample were equalized.</p
Additional file 10 of Identification of crucial genes and metabolites regulating the eggshell brownness in chicken
Additional file 10: Fig. S2. The location of the next ovulated egg in the oviduct at different time points following oviposition