STEPPING CHARACTERISTICS BEFORE STAIR WALKING TRANSITONS IN TAICHI ELDERLY

The purpose of this study was to investigate the difference between TC exerciser and normal elderly in stepping characteristics before stair walking transition. There were 12 TC practitioner elderly and 14 matched controls participated in this stady. Ten Vicon high-speed cameras (250Hz) were utilised to collect kinematic data. Results showed that TC group presented faster CoM velocity during descending and following walk. At the moment of just before transition, TC group showed faster resultant / horizontal CoM velocity, TOE resultant / vertical velocity. TC group also demonstrated greater stride length while contacting the ground. We concluded TC group had better abilities of body control. Faster horizontal CoM velocity and vertical TOE velocity negotiated before transiton in TC group, would be order to transit the unstable situation more efficient

AGE-DIFFERENCES IN THE FREE VERTICAL MOMENTS DURING STEPPING DOWN - PILOT

The aim of this study was to understand age-differences in body control during stepping down by investigating free vertical moments (FVMs). Two older adults and two young adults participated in this study. During each trial, lower extremities kinematics were measured using a 10 camera Vicon system (250Hz) and ground reaction forces were recorded using two Kistler force platforms (1000Hz). FVM was calculated by ground reaction forces using Visual3D software. The results indicated young adults showed adduction-FVM (ADD-FVM) but older adults presented abduction-FVM (ABD-FVM) during double-stance phase. Older adults seemed to exert more ABD-FVM than young adults while in the single support phase. It was concluded that the FVMs seemed to point to different strategies between older adults and young adults

BIOMECHANICAL DIFFERENCES BETWEEN TAI-CHI PRACTICING AND ACTIVE ELDERLY DURING THE STAIR-TO-FLOOR TRANSITION

Long-term Tai-Chi practitioners tend to have similar movement to healthy adults and exhibit movement strategies that reduce fall risk during stair-to-floor transition. We aimed to assess the differences during stair descent to ground in Tai-Chi elderly practitioners and active elderly. Fourteen regular Tai-Chi practitioners and fourteen active elderly participated. Whole-body kinematics and ground reaction forces (GRFs) were recorded synchronously by using motion analysis and a force platform. A t-test was used to test the differences between the groups. Both descent and forward walking step length and center of mass (COM) velocity, both horizontal braking and propulsive force and impulse, ankle range of motion (ROM) and total work in the sagittal plane, and maximum hip moment in the frontal plane had significant differences. Our results appear to support the benefits of long-term Tai-Chi training during the stair-to-floor transition

SAGITTAL AND FRONTAL LOWER LIMBS KINETICS DURING STEPPING DOWN IN TAICHI ELDERLY

The aim was to compare the kinetic characteristics of the beginning stance phase during stepping down in Taichi and normal elderly. Nine elderly taichi subjects and eleven matched controls participated in the study. Whole body kinematics and ground reaction forces (GRF) were recorded using 10 Vicon cameras (250Hz) and two Kistler force plates (1000Hz). Sagittal and frontal kinetic parameters were calculated by using Visual3D software. Differences in variables between groups were tested using t-test. The results indicated hip extensor / knee flexor / ankle plantarflexor / support moment and peak hip/knee/ankle power were greater in Taichi group. It was concluded that Taichi group has ability to translate forward movement (hip moment / power), to control body (knee moment /power) and to absorption energy (ankle moment / power) in sagittal plane

Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells

Type-2 ribosome-inactivating proteins, composed of a toxic A-chain and lectin-like B-chain, display various biological functions, including cytotoxicity and immunomodulation. We here cloned the lectin-like B-chain encoding fragment of a newly identified type-2 RIP gene, articulatin gene, from Viscum articulatum, into a bacterial expression vector to obtain nonglycosylated recombinant protein expressed in inclusion bodies. After purification and protein refolding, soluble refolded recombinant articulatin B-chain (rATB) showed lectin activity specific toward galactoside moiety and was stably maintained while stored in low ionic strength solution. Despite lacking glycosylation, rATB actively bound leukocytes with preferential binding to monocytes and in vitro stimulated PBMCs to release cytokines without obvious cytotoxicity. These results implicated such a B-chain fragment as a potential immunomodulator

Role of pirenoxine in the effects of catalin on in vitro ultraviolet-induced lens protein turbidity and selenite-induced cataractogenesis in vivo

Purpose: In this study, we investigated the biochemical pharmacology of pirenoxine (PRX) and catalin under in vitro selenite/calcium- and ultraviolet (UV)-induced lens protein turbidity challenges. The systemic effects of catalin were determined using a selenite-induced cataractogenesis rat model. Methods: In vitro cataractogenesis assay systems (including UVB/C photo-oxidation of lens crystallins, calpain-induced proteolysis, and selenite/calcium-induced turbidity of lens crystallin solutions) were used to screen the activity of PRX and catalin eye drop solutions. Turbidity was identified as the optical density measured using spectroscopy at 405 nm. We also determined the in vivo effects of catalin on cataract severity in a selenite-induced cataract rat model. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) was applied to analyze the integrity of crystallin samples. Results: PRX at 1,000 μM significantly delayed UVC-induced turbidity formation compared to controls after 4 h of UVC exposure (p<0.05), but not in groups incubated with PRX concentrations of <1,000 μM. Results were further confirmed by SDS–PAGE. The absolute γ-crystallin turbidity induced by 4 h of UVC exposure was ameliorated in the presence of catalin equivalent to 1~100 μM PRX in a concentration-dependent manner. Samples with catalin-formulated vehicle only (CataV) and those containing PRX equivalent to 100 μM had a similar protective effect after 4 h of UVC exposure compared to the controls (p<0.05). PRX at 0.03, 0.1, and 0.3 μM significantly delayed 10 mM selenite- and calcium-induced turbidity formation compared to controls on days 0~4 (p<0.05). Catalin (equivalent to 32, 80, and 100 μM PRX) had an initial protective effect against selenite-induced lens protein turbidity on day 1 (p<0.05). Subcutaneous pretreatment with catalin (5 mg/kg) also statistically decreased the mean cataract scores in selenite-induced cataract rats on post-induction day 3 compared to the controls (1.3±0.2 versus 2.4±0.4; p<0.05). However, catalin (equivalent to up to 100 μM PRX) did not inhibit calpain-induced proteolysis activated by calcium, and neither did 100 μM PRX. Conclusions: PRX at micromolar levels ameliorated selenite- and calcium-induced lens protein turbidity but required millimolar levels to protect against UVC irradiation. The observed inhibition of UVC-induced turbidity of lens crystallins by catalin at micromolar concentrations may have been a result of the catalin-formulated vehicle. Transient protection by catalin against selenite-induced turbidity of crystallin solutions in vitro was supported by the ameliorated cataract scores in the early stage of cataractogenesis in vivo by subcutaneously administered catalin. PRX could not inhibit calpain-induced proteolysis activated by calcium or catalin itself, and may be detrimental to crystallins under UVB exposure. Further studies on formulation modifications of catalin and recommended doses of PRX to optimize clinical efficacy by cataract type are warranted

Mediating role of resilience in the relationships of physical activity and mindful self-awareness with peace of mind among college students

Abstract Peace of mind (PoM) is an index of mental health in Asian culture and emphasizes low arousal, happiness, harmony, and an internal state of peacefulness. While previous studies have found that mindful self-awareness can contribute to PoM, regular physical activity (PA) is also an important factor contributing to one’s PoM due to its function in promoting one's resilience. The study aims to investigate a hypothetical model that assumes PA is associated with resilience while controlling for mindful self-awareness, contributing to PoM. The PoM scale, Connor-Davidson Resilience Scale, Chinese translation of Mindful Attention Awareness Scale, and PA self-report questionnaire were used. A path analysis was applied to test the association between these variables and the mediating role of resilience. A total of 436 students from a university in Taiwan were recruited; the mean age was 20.87, with 46.3% female and 73.6% engaging in over 150 min/week of moderate PA. Gender and age negatively correlated with PA. After controlling for age and gender, there was no direct effect of physical activity on PoM; both mindful self-awareness and PA predict resilience, which in turn predicts PoM, suggesting that both cognitive (i.e., mindful self-awareness) and PA are important to cultivate resilience and thus PoM