Efficient and Provably-secure Certificateless Strong Designated Verifier Signature Scheme without Pairings

Strong designated verifier signature (generally abbreviated to SDVS) allows signers to obtain absolute control over who can verify the signature, while only the designated verifier other than anyone else can verify the validity of a SDVS without being able to transfer the conviction. Certificateless PKC has unique advantages comparing with certificate-based cryptosystems and identity-based PKC, without suffering from key escrow. Motivated by these attractive features, we propose a novel efficient CL-SDVS scheme without bilinear pairings or map-to-point hash operations. The proposed scheme achieves all the required security properties including EUF-CMA, non-transferability, strongness and non-delegatability. We also estimate the computational and communication efficiency. The comparison shows that our scheme outperforms all the previous CL-(S)DVS schemes. Furthermore, the crucial security properties of the CL-SDVS scheme are formally proved based on the intractability of SCDH and ECDL assumptions in random oracle model

Echinacoside attenuates lipopolysaccharide-induced acute lung injury in newborn mice via inactivation of NF- κB/NLRP3 signaling pathway

Purpose: To investigate the effect of echinacoside (ECH) on acute lung injury (ALI) and the underlying mechanism of action.Methods: The ALI model was established through intranasal instillation of lipopolysaccharide (LPS). Lung tissue damage was determined using hematoxylin and eosin (H&E) staining and lung wet-to-dry–weight ratio. Bronchoalveolar lavage fluid (BALF) protein concentration, cell count, and cytokine level were evaluated. Western blotting was used to determine protein expression level.Results: ECH attenuated lung tissue injury and lung wet-to-dry–weight ratio in the ALI model (p < 0.01). The total protein content and number of total cells, neutrophils, and macrophages increased in BALF of mice treated with LPS, but these increases were reversed by ECH treatment (p < 0.01). The levels of TNF-α and IL-1β increased in BALF and lung tissue of LPS-treated mice; however, ECH treatment decreased these changes (p < 0.01). In addition, ECH inhibited the activation of the nuclear factor-κB (NF-κB)/NLR family pyrin domain containing 3 (NLRP3) pathway in LPS-treated mice (p < 0.01).Conclusion: Echinacoside attenuates LPS-induced ALI via inactivation of the NF-κB/NLRP3 pathway, making echinacoside a potential drug for the treatment of ALI. Keywords: Echinacoside, Acute lung injury, Lipopolysaccharide, Nuclear factor-κB, NLR family pyrin domain containing

Gut Bacterial Communities of Lymantria xylina and Their Associations with Host Development and Diet

The gut microbiota of insects has a wide range of effects on host nutrition, physiology, and behavior. The structure of gut microbiota may also be shaped by their environment, causing them to adjust to their hosts; thus, the objective of this study was to examine variations in the morphological traits and gut microbiota of Lymantria xylina in response to natural and artificial diets using high-throughput sequencing. Regarding morphology, the head widths for larvae fed on a sterilized artificial diet were smaller than for larvae fed on a non-sterilized host-plant diet in the early instars. The gut microbiota diversity of L. xylina fed on different diets varied significantly, but did not change during different development periods. This seemed to indicate that vertical inheritance occurred in L. xylina mutualistic symbionts. Acinetobacter and Enterococcus were dominant in/on eggs. In the first instar larvae, Acinetobacter accounted for 33.52% of the sterilized artificial diet treatment, while Enterococcus (67.88%) was the predominant bacteria for the non-sterilized host-plant diet treatment. Gut microbe structures were adapted to both diets through vertical inheritance and self-regulation. This study clarified the impacts of microbial symbiosis on L. xylina and might provide new possibilities for improving the control of these bacteria

Unraveling clonal CD8 T cell expansion and identification of essential factors in γ-herpesvirus-induced lymphomagenesis

Alcelaphine gammaherpesvirus 1 (AlHV-1) asymptomatically persists in its natural host, the wildebeest. However, cross-species transmission to cattle results in the induction of an acute and lethal peripheral T cell lymphoma-like disease (PTCL), named malignant catarrhal fever (MCF). Our previous findings demonstrated an essential role for viral genome maintenance in infected CD8+ T lymphocytes but the exact mechanism(s) leading to lymphoproliferation and MCF remained unknown. To decipher how AlHV-1 dysregulates T lymphocytes, we first examined the global phenotypic changes in circulating CD8+ T cells after experimental infection of calves. T cell receptor repertoire together with transcriptomics and epigenomics analyses demonstrated an oligoclonal expansion of infected CD8+ T cells displaying effector and exhaustion gene signatures, including GZMA, GNLY, PD-1, and TOX2 expression. Then, among viral genes expressed in infected CD8+ T cells, we uncovered A10 that encodes a transmembrane signaling protein displaying multiple tyrosine residues, with predicted ITAM and SH3 motifs. Impaired A10 expression did not affect AlHV-1 replication in vitro but rendered AlHV-1 unable to induce MCF. Furthermore, A10 was phosphorylated in T lymphocytes in vitro and affected T cell signaling. Finally, while AlHV-1 mutants expressing mutated forms of A10 devoid of ITAM or SH3 motifs (or both) were able to induce MCF, a recombinant virus expressing a mutated form of A10 unable to phosphorylate its tyrosine residues resulted in the lack of MCF and protected against a wild-type virus challenge. Thus, we could characterize the nature of this γ-herpesvirus-induced PTCL-like disease and identify an essential mechanism explaining its development.</p

Whole-genome sequencing of <em>Oryza brachyantha</em> reveals mechanisms underlying <em>Oryza</em> genome evolution

The wild species of the genus Oryza contain a largely untapped reservoir of agronomically important genes for rice improvement. Here we report the 261-Mb de novo assembled genome sequence of Oryza brachyantha. Low activity of long-terminal repeat retrotransposons and massive internal deletions of ancient long-terminal repeat elements lead to the compact genome of Oryza brachyantha. We model 32,038 protein-coding genes in the Oryza brachyantha genome, of which only 70% are located in collinear positions in comparison with the rice genome. Analysing breakpoints of non-collinear genes suggests that double-strand break repair through non-homologous end joining has an important role in gene movement and erosion of collinearity in the Oryza genomes. Transition of euchromatin to heterochromatin in the rice genome is accompanied by segmental and tandem duplications, further expanded by transposable element insertions. The high-quality reference genome sequence of Oryza brachyantha provides an important resource for functional and evolutionary studies in the genus Oryza

Evaluation of Global Historical Cropland Datasets with Regional Historical Evidence and Remotely Sensed Satellite Data from the Xinjiang Area of China

Global land use/cover change (LUCC) datasets are essential for quantitatively assessing the impacts of LUCC on global change, but many uncertainties in existing global datasets seriously hamper climate modeling. Evaluating the reliability of existing global LUCC datasets is a precondition for improved data quality. In this study, based on the regional historical document-based reconstructions, satellite-based data, and historical reclamation evidence for the Xinjiang area of China, the accuracy and rationality of cropland data for this area in the HYDE 3.2 and SAGE datasets were evaluated by utilizing comparative analysis regarding three aspects, namely the change tendency of the cropland area, the area of cropland, and the differences in spatial pattern. This study concluded that the amount of cropland in the Xinjiang area in the global and regional datasets shows both disparate trends and large differences in absolute values. Spatially, historical reclamation evidence indicated that agricultural cultivation in the Xinjiang area underwent expansion from south to north and from east to west over the past 300 years; however, the global datasets revealed that the cropland spatial patterns in the Xinjiang area in the historical period are similar to those in the current period. These differences are attributable to the uncertainties of the basic assumptions, per capita cropland area estimates, and reconstruction methods in the global datasets. The findings of the study highlight the necessity of regional studies on historical LUCC in the Xinjiang area

Inconsistent Carbon Budget Estimation Using Dynamic/Static Carbon Density under Land Use and Land Cover Change: A Case Study in Henan Province, China

The scientific estimation of carbon budgets induced by land use and land cover change (LUCC) can improve the accuracy of carbon budget estimates for terrestrial ecosystems and deepen our understanding of the future carbon sink potential of these ecosystems. Previous studies have typically selected one of many LUCC-induced carbon budget methods and overlooked the differences in the results between the different methods. Taking Henan Province, China, as a case study, we used the IPCC method, the bookkeeping model, and the inventory method to estimate LUCC-induced carbon budgets and compared the differences in these methods. The results showed that LUCC in Henan Province was categorized as a carbon sink from 1980 to 2015 based on the IPCC method and the inventory method. However, the estimates were significantly different, and the total estimates of the IPCC method were 40% less than those of the inventory method. At the regional ecogeographical scale, the two methods were not consistent in assessing the carbon source/sink of LUCC. The bookkeeping model categorized LUCC as a carbon source in Henan Province for the same period, and the carbon storage change trend opposed those of the other two methods. The failure of the IPCC method and the bookkeeping model to include the dynamic changes in carbon density caused by climate and land use management led to the great differences between the three methods. The inventory method is recommended to estimate the carbon budget caused by LUCC for regions where carbon density varies greatly over time. Both the IPCC method and the bookkeeping model may have great uncertainty in estimating changes in LUCC-induced carbon stocks and should be used with caution

The ultrasonic features and diagnostic performance of ultrasound for ovarian cystadenofibroma and adenofibroma

Objective: To investigate ultrasonic features of ovarian cystadenofibroma (CAF) and adenofibroma (AF). Methods: Ultrasonic features of 50 cases with ovarian CAF and 7 cases with AF were analyzed, and diagnostic performance of ultrasound was evaluated. Results: According to pathological findings of the CAF cases studied, 46 were benign CAF with 47 tumors identified, and 4 cases were borderline AF. All the seven AF cases were benign with single lesion. Pathologically, most of lesions was serous (53/58, 91.4%). With borderline CAF defined as malignant, the ultrasound differentiated benign from malignant masses of CAF and AF with a sensitivity of 100.0% (95%CI 39.8%-100.0%), specificity of 72.2% (95%CI 58.4%-83.5%), and accuracy of 74.1%. Most benign CAF masses were described as unilocular solid cysts (25/47, 53.2%) with regular morphology (43/47, 91.5%) and well-defined boundaries (46/47, 97.9%). Regular papillary projections (32/47, 68.1%) in CAF lesions manifested no or minimal color Doppler signals（31/32, 96.9%）. The borderline CAF masses were all identified with solid components or papillary projections, which manifested various degree of blood supply. Compared with benign CAF, borderline CAF showed more solid or papillary components [maximum diameter, borderline CAF was 19.5 (18.8-26.3) mm versus benign 8.0 (7.0-13) mm, P=0.002], with higher the maximum diameter ratio of the solid components or papillary projections to the mass [the borderline CAF was 0.35 versus benign CAF 0.15, P=0.033], and there were more blood supply inside the borderline CAF (4/4) than benign CAF (10/35), (P=0.012). Most of the AF masses were described as regular (6/7), well-defined (5/7) solid masses with shadowing identified (4/7). Absent color Doppler signals were recorded in the solid components (6/6). Conclusions: The ultrasound has a sound diagnostic performance for CAF and AF. Benign CAF lesions are mostly described as solid cysts with papillary projection(s) manifesting no or minimal color Doppler signals; borderline CAFs are mostly described as masses with papillary projection(s) and solid component(s) containing varied blood supply. AFs are mostly described as solid masses with shadowing identified and absent color Doppler signals. It is difficult to discriminate benign from borderline CAF, presence of solid components or papillary projections and copious blood supply may indicate borderline CAF

Inconsistent Carbon Budget Estimation Using Dynamic/Static Carbon Density under Land Use and Land Cover Change: A Case Study in Henan Province, China

The scientific estimation of carbon budgets induced by land use and land cover change (LUCC) can improve the accuracy of carbon budget estimates for terrestrial ecosystems and deepen our understanding of the future carbon sink potential of these ecosystems. Previous studies have typically selected one of many LUCC-induced carbon budget methods and overlooked the differences in the results between the different methods. Taking Henan Province, China, as a case study, we used the IPCC method, the bookkeeping model, and the inventory method to estimate LUCC-induced carbon budgets and compared the differences in these methods. The results showed that LUCC in Henan Province was categorized as a carbon sink from 1980 to 2015 based on the IPCC method and the inventory method. However, the estimates were significantly different, and the total estimates of the IPCC method were 40% less than those of the inventory method. At the regional ecogeographical scale, the two methods were not consistent in assessing the carbon source/sink of LUCC. The bookkeeping model categorized LUCC as a carbon source in Henan Province for the same period, and the carbon storage change trend opposed those of the other two methods. The failure of the IPCC method and the bookkeeping model to include the dynamic changes in carbon density caused by climate and land use management led to the great differences between the three methods. The inventory method is recommended to estimate the carbon budget caused by LUCC for regions where carbon density varies greatly over time. Both the IPCC method and the bookkeeping model may have great uncertainty in estimating changes in LUCC-induced carbon stocks and should be used with caution