Parameter Setting Strategy for the Controller of the DFIG Wind Turbine Considering the Small-Signal Stability of Power Grids

Due to the increasing penetration of the wind generation, the stability, especially the small-signal stability, of the power grid is much related to it. Currently, few studies considered the impact of the parameter settings of the wind turbine controller on the small-signal stability of the grid under the full range of wind conditions. In this paper, we propose a framework for deriving a set of controller parameters by interiorizing their impact on the power system stability, based on an analytic model of a 15th-order single DFIG-infinite grid connection under all wind speeds. The study results on a real wind turbine show that the controller parameters optimized for a specific wind speed may not feasible for other operational conditions yet the proposed framework can obtain a set of parameters guaranteeing the power system stability under all wind speeds

Slug down-regulation by RNA interference inhibits invasion growth in human esophageal squamous cell carcinoma

<p>Abstract</p> <p>Background</p> <p>Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive carcinomas of the gastrointestinal tract. We assessed the relevance of Slug in measuring the invasive potential of ESCC cells <it>in vitro </it>and <it>in vivo </it>in immunodeficient mice.</p> <p>Methods</p> <p>We utilized RNA interference to knockdown Slug gene expression, and effects on survival and invasive carcinoma were evaluated using a Boyden chamber transwell assay <it>in vitro</it>. We evaluated the effect of Slug siRNA-transfection and Slug cDNA-transfection on E-cadherin and Bcl-2 expression in ESCC cells. A pseudometastatic model of ESCC in immunodeficient mice was used to assess the effects of Slug siRNA transfection on tumor metastasis development.</p> <p>Results</p> <p>The EC109 cell line was transfected with Slug-siRNA to knockdown Slug expression. The TE13 cell line was transfected with Slug-cDNA to increase Slug expression. EC109 and TE13 cell lines were tested for the expression of apoptosis-related genes bcl-2 and metastasis-related gene E-cadherin identified previously as Slug targets. Bcl-2 expression was increased and E-cadherin was decreased in Slug siRNA-transfected EC109 cells. Bcl-2 expression was increased and E-cadherin was decreased in Slug cDNA-transfected TE13 cells. Invasion of Slug siRNA-transfected EC109 cells was reduced and apoptosis was increased whereas invasion was greater in Slug cDNA-transfected cells. Animals injected with Slug siRNA-transfected EC109 cells exhihited fewer seeded nodes and demonstrated more apoptosis.</p> <p>Conclusions</p> <p>Slug down-regulation promotes cell apoptosis and decreases invasion capability <it>in vitro </it>and <it>in vivo</it>. Slug inhibition may represent a novel strategy for treatment of metastatic ESCC.</p

Conserved synteny at the protein family level reveals genes underlying Shewanella species’ cold tolerance and predicts their novel phenotypes

© The Authors 2009. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License. The definitive version was published in Functional & Integrative Genomics 10 (2010): 97-110, doi:10.1007/s10142-009-0142-y.Bacteria of the genus Shewanella can thrive in different environments and demonstrate significant variability in their metabolic and ecophysiological capabilities including cold and salt tolerance. Genomic characteristics underlying this variability across species are largely unknown. In this study, we address the problem by a comparison of the physiological, metabolic, and genomic characteristics of 19 sequenced Shewanella species. We have employed two novel approaches based on association of a phenotypic trait with the number of the trait-specific protein families (Pfam domains) and on the conservation of synteny (order in the genome) of the trait-related genes. Our first approach is top-down and involves experimental evaluation and quantification of the species’ cold tolerance followed by identification of the correlated Pfam domains and genes with a conserved synteny. The second, a bottom-up approach, predicts novel phenotypes of the species by calculating profiles of each Pfam domain among their genomes and following pair-wise correlation of the profiles and their network clustering. Using the first approach, we find a link between cold and salt tolerance of the species and the presence in the genome of a Na+/H+ antiporter gene cluster. Other cold-tolerance-related genes include peptidases, chemotaxis sensory transducer proteins, a cysteine exporter, and helicases. Using the bottom-up approach, we found several novel phenotypes in the newly sequenced Shewanella species, including degradation of aromatic compounds by an aerobic hybrid pathway in Shewanella woodyi, degradation of ethanolamine by Shewanella benthica, and propanediol degradation by Shewanella putrefaciens CN32 and Shewanella sp. W3-18-1.This research was supported by the U.S. Department of Energy (DOE) Office of Biological and Environmental Research under the Genomics: GTL Program via the Shewanella Federation consortium

Ground Simulation Test of 2D Dynamic Overload Environment of Fuze Launching

The fuze launch process is subjected to backseat and spin overloads. To address this issue, a loading method of a 2D dynamic acceleration environment was developed in this study for testing fuze antioverload performance on ground. The techniques of flywheel energy storage, high-speed impact, and centrifugal rotation in the track are combined in a dynamic analysis and simulation. First, the flywheel is rotated at a constant speed by a variable-frequency motor to obtain high kinetic energy. Second, an impact hammer is instantaneously released on the specimen at a high speed, loading the backseat acceleration environment. Finally, the impact hammer is retracted, and the specimen is rotated in the track instead of spinning around its axis, thereby loading the centrifugal acceleration environment. The peak value and pulse width of the 2D overload acceleration can be adjusted by changing the speed of the flywheel and buffers in the abovementioned process. The experimental and simulation results observed that the peak value of backseat acceleration could reach 34,559 g, the pulse width was approximately 400 μs, and the peak value of the centrifugal acceleration was 1,020 g. The study results showed that the proposed approach fulfills the requirements of the 2D overload simulation test of the micro-electromechanical system (MEMS) fuze safety and arming mechanism. The proposed loading method has been successfully applied to ground simulation tests of the MEMS fuze safety and arming mechanism

The Effect of Multi-Years Reclaimed Water Irrigation on Dryland Carbon Sequestration in the North China Plain

Reclaimed water is an alternative water source which could alleviate the shortage of water resources in agricultural systems. Many researchers have studied the effect of reclaimed water on soil environment, crop yield, etc. However, carbon sequestration in reclaimed water irrigated agricultural systems is less studied. This study investigates methane uptake and photosynthesis in reclaimed water irrigation systems contributing to carbon sequestration estimation and analyzes the important factors impacting them. The results show that CH4 uptake is related to soil water-filled pore space (WFPS) with a quadratic and it has the highest uptake when WFPS is between 40 and 50%. Long-term reclaimed water irrigation could significantly decrease (p &lt; 0.05) CH4 uptake and macroaggregate stability in the topsoil. However, reclaimed water had no significant impact on photosynthesis in comparison. The type of fertilizer is an important factor which impacts CH4 emission from soil; urea had a lower CH4 uptake and a higher CO2 emission than slow-released fertilizer. Overall, reclaimed water irrigation could effectively decrease soil carbon sequestration. A soil wetted proportion level of 40–50% was recommended in this study for favorable methane oxidation. Slow-released fertilizer in reclaimed water irrigated agriculture could better control soil carbon emission and soil carbon absorption

Machinability and Surface Generation of Pd40Ni10Cu30P20 Bulk Metallic Glass in Single-Point Diamond Turning

Pd40Ni10Cu30P20 bulk metallic glass (BMG) is widely used in industrial fields due to its excellent oxidation resistance, corrosion resistance, and thermal stability. However, the lack of research on the machinability and cutting performance of BMG using single-point diamond turning (SPDT) limits its application for engineering manufacturing. In the present research, a series of turning experiments were carried out under different cutting parameters, and the machinability reflected by the quality of machined surface, chip morphology, and tool wear were analyzed. Based on the oxidation phenomenon of the machined surface, a molecular dynamics (MD) simulation was conducted to study the mechanism and suppression of the machined surface oxidation during the cutting. The results show that: (1) The Pd-based BMG had good machinability, where the machined surface roughness could go down to 3 nm; (2) irregular micro/nanostructures were found along the tool path on the outer circular region of the machined surface, which greatly affected the surface roughness; and (3) the cutting heat softened the workpiece material and flattened the tool marks under surface tension, which improved the surface quality. This research provides important theoretical and technical support for the application of BMG in optical mold manufacturing