Visible-Light-Induced Intramolecular C(sp²)–H Amination and Aziridination of Azidoformates via a Triplet Nitrene Pathway

Catalytic intramolecular C–H amination and aziridination reactions of <i>o</i>-allylphenyl azidoformates have been achieved under visible-light irradiation, providing a mild, clean, and efficient method for the synthesis of useful benzoxazolones and [5.1.0] bicyclic aziridines. Mechanistic studies suggest that a triplet nitrene acts as the reactive intermediate. The chemoselectivity of the reaction, with alkyl olefin aziridination ≫ electron deficient olefin aziridination ≈ C­(sp²)–H amination ≫ C­(sp³)–H amination was observed, which may be instructive in the development of an understanding of visible-light-induced triplet nitrene transformation reactions

Summary of the cDNA library of goose peripheral blood lymphocytes.

<p>Summary of the cDNA library of goose peripheral blood lymphocytes.</p

Histogram presentation of eukaryotic cluster of orthologous groups (KOG) classification.

<p>A total of 15,334 sequences were annotated into 25 KOG categories.</p

Amino acid alignment of complement C1q subcomponent subunit A (C1qA).

<p>Amino acid alignment of C1qA shows, signal peptide, collagen like domain and C1q domain are respectively indicated with a bottle green arrow, orange arrow and blue arrow. Blue Triangle indicates the key residues for CRP interaction. Purple Triangle indicates the key residues for IgG interaction. Magenta pentacle indicates the sites for calcium binding. Residues in the cyan rectangle are in the formation of inter-chain disulfide bond. Residues with the hollow blue triangle are O-linked glycans sites in post-translation. Residues in yellow-green rectangle are hydroxylation sites with GXPG motif. NCBI accession numbers of C1qAs are listed as follows: goose: KP238277; duck: 514725938; bird (zebra finch): 449487169; chicken: 118101238; lizard: XP_003230642.1; mouse: 408359988; human: 399138.</p

Insight into the Role of Hydrogen Bonding in the Molecular Self-Assembly Process of Sulfamethazine Solvates

The new solid forms screening of sulfamethazine was conducted in 16 kinds of different pure solvents. Four new sulfamethazine solvates were reported for the first time, and three crystal structures of solvates were successfully determined from single-crystal X-ray diffraction data. The results showed that sulfamethazine solvate formation directly depended on the solvents used in the experiments. The solvent properties were used to evaluate the effects of solvent on solvate formation. It was found that the H-bond acceptor ability of the solvent was the main factor that governed the solvate formation. The H-bonded motifs in the structures of solvates have been fully characterized. The results revealed that sulfamethazine solvate formation was mainly driven by molecular self-assembly through hydrogen bonding between solvent and solute molecules. Meanwhile, the crystal structures results also showed that the sulfamethazine molecule had flexible conformation. Furthermore, the principles of different sulfamethazine molecules packing in different crystal structures were discussed from the view of molecular intermolecular interactions and the molecular conformation

sj-pdf-1-imr-10.1177_03000605221121940 - Supplemental material for Type 1 diabetes mellitus induced by PD-1 inhibitors in China: a report of two cases

Supplemental material, sj-pdf-1-imr-10.1177_03000605221121940 for Type 1 diabetes mellitus induced by PD-1 inhibitors in China: a report of two cases by Jingmei Luo, Jiagang Feng, Chunyan Liu, Zhongce Yang, Dong Zhan, Yanan Wu, Li Pan and Lihua Zhang in Journal of International Medical Research</p

Comparison of goose immune relevant genes with duck, chicken, turkey and zebra finch.

<p>The number of immune relevant genes of goose and its comparison among the known sequences of duck, chicken, turkey and zebra finch deposited in the NCBI database. (a) represents the total number of immune relevant genes found in the goose, (b-e) represents the immune relevant genes found in duck, chicken, turkey, and zebra finch, and (f-i) represents the immune relevant genes not found in the duck, chicken, turkey, and zebra finch.</p

Amino acid alignment of suppressor of cytokine signaling 3 (SOCS3).

<p>Amino acid alignment of SOCS3 shows the SH2_SOCS3 domain and SOCS_SOCS3 box are respectively indicated by orange arrow and pink arrow. An extended SH2 subdomain is marked with dark green line. Kinase inhibitory region (KIR) is masked with cyan box. Functional sites are marked with blue triangles. L22, F25, E30, Y31, V34, L41, G45 and R71 have an effect on EPO/LIF-induced signaling suppression. L58, L93 and R94 have an effect on the binding to Y429/Y431 phosphorylated EPOR. NCBI accession numbers of SOCS3s are listed as follows: goose: KP238281; duck: 514711433; chicken: 45382967; bird (zebra finch): 224074414; lizard: 637266413; mouse: 6671758; human: 49168482;</p

<i>De Novo</i> Transcriptomic Analysis of Peripheral Blood Lymphocytes from the Chinese Goose: Gene Discovery and Immune System Pathway Description

<div><p>Background</p><p>The Chinese goose is one of the most economically important poultry birds and is a natural reservoir for many avian viruses. However, the nature and regulation of the innate and adaptive immune systems of this waterfowl species are not completely understood due to limited information on the goose genome. Recently, transcriptome sequencing technology was applied in the genomic studies focused on novel gene discovery. Thus, this study described the transcriptome of the goose peripheral blood lymphocytes to identify immunity relevant genes.</p><p>Principal Findings</p><p><i>De novo</i> transcriptome assembly of the goose peripheral blood lymphocytes was sequenced by Illumina-Solexa technology. In total, 211,198 unigenes were assembled from the 69.36 million cleaned reads. The average length, N50 size and the maximum length of the assembled unigenes were 687 bp, 1,298 bp and 18,992 bp, respectively. A total of 36,854 unigenes showed similarity by BLAST search against the NCBI non-redundant (Nr) protein database. For functional classification, 163,161 unigenes were comprised of three Gene Ontology (Go) categories and 67 subcategories. A total of 15,334 unigenes were annotated into 25 eukaryotic orthologous groups (KOGs) categories. Kyoto Encyclopedia of Genes and Genomes (KEGG) database annotated 39,585 unigenes into six biological functional groups and 308 pathways. Among the 2,757 unigenes that participated in the 15 immune system KEGG pathways, 125 of the most important immune relevant genes were summarized and analyzed by STRING analysis to identify gene interactions and relationships. Moreover, 10 genes were confirmed by PCR and analyzed. Of these 125 unigenes, 109 unigenes, approximately 87%, were not previously identified in the goose.</p><p>Conclusion</p><p>This <i>de novo</i> transcriptome analysis could provide important Chinese goose sequence information and highlights the value of new gene discovery, pathways investigation and immune system gene identification, and comparison with other avian species as useful tools to understand the goose immune system.</p></div

List of annotations against public databases.

<p>List of annotations against public databases.</p