Isolation and amino acid sequence of a mating pheromone produced by mating type α cells of Saccharomyces kluyveri

AbstractA peptide, termed αsk2 pheromone, was isolated from culture filtrates of mating type α cells of Saccharomyces kluyveri as a mating pheromone, having both shmoo inducing (in a cells of S. kluyveri and S. cerevisiae) and agglutinability inducing (in a cells of S. cerevisiae) actions. The amino acid sequence of αsk2 pheromone was determined as H—Trp—His—Trp—Leu—Ser—Phe—Ser—Lys—Gly—Glu—Pro—Met—Tyr-OH by mass spectrometry, sequence analysis and enzymatic digestions