A Phase Change Storage Material that May be Used in the Fire Resistance of Building Structure

AbstractThis study prepared polyethylene glycol/silicon dioxide composite, a kind of form-stable phase change material. The composites can be made into mortar which is able to adhere to the surface of building structure and absorb the fire heat. This paper aims to study the effect of the composites on the fire resistance of building structure. Scanning electronic microscope and differential scanning calorimeter were adopted to investigate the structural and thermal properties of the composites. It was found that the polyethylene glycol was well dispersed into the network of solid SiO2. And the latent heat of PEG/SiO2 increased with the decrease of SiO2 content. The required weight percentage of SiO2 was found to be 15% at least if the composites remain solid without leakage. It was also found that a phase change of pure PEG6000 happened with an enthalpy of 158J/g while the 80 wt% PEG composite is 133J/g. In conclusion, the phase change storage material may be used for fire resistance of building structure

Spectral fluctuations in the interacting boson model

The energy dependence of the spectral fluctuations in the interacting boson model (IBM) and its connections to the mean-field structures have been analyzed through adopting two statistical measures, the nearest neighbor level spacing distribution $P(S)$ measuring the chaoticity (regularity) in energy spectra and the $\Delta_3(L)$ statistics of Dyson and Metha measuring the spectral rigidity. Specifically, the statistical results as functions of the energy cutoff have been worked out for different dynamical situations including the U(5)-SU(3) and SU(3)-O(6) transitions as well as those near the AW arc of regularity. It is found that most of the changes in spectral fluctuations are triggered near the stationary points of the classical potential especially for the cases in the deformed region of the IBM phase diagram. The results thus justify the stationary point effects from the point of view of statistics. In addition, the approximate degeneracies in the $2^+$ spectrum on the AW arc is also revealed from the statistical calculations

苏州市三甲医院医患关系现状及其影响因素分析*

Objective: To investigate the relationship between doctors and patients in Suzhou, we focused on exploring the factors of doctor-patient communication, and strived to deepen the doctor-patient communication skills and knowledge. Method: Questionnaire survey was carried out in comprehensive tertiary-class hospitals in Suzhou , adopting the method of random sampling, respectively on patients and doctors. Results: 593 valid questionnaires were from both doctors and patients. The doctors thought that the current doctor-patient relationship  "good" and above accounted for 32% (31/98).At the meanwhile, in the patients, this proportion was 45% (223/495).There was statistically significance between the difference(P ＜0.05).Only 6% doctors thought that the communication between doctors and patients is not important; in the patients, the ratio was 10%. Among the doctors, the top three factors of doctor-patient communication were: lack of communication skills, too much tasks and not enough time and energy, not good attitude. Among patients, the top three factors were: incomprehension and distrust of the doctors, the poor understanding for medical knowledge and the low cultural level. Conclusion: In the first-class hospitals of Suzhou, the relationship between doctors and patients had a relatively good development trend. There were some problems in the communication between doctors and patients. We should enhance the doctor-patient communication, and build a harmonious doctor-patient relationship.目的  了解苏州市三甲医院的医患关系现状，探寻医患沟通的影响因素，以增进医患沟通的知识及技能。方法  在苏州市综合性“三甲”医院进行问卷调查，采用单纯随机抽样方法，分别就患方和医方进行调研。结果  收回医患双方有效问卷593份，有效率为94.6%。医方认为当前医患关系“比较好”及以上者占32%(31/98)，患方认为当前医患关系“比较好”及以上者占45%(223/495)，两者比较差异有统计学意义（P＜0.05）。医方有6%的人认为医患沟通不重要；在患方，这一比率为10%。就医方责任方面来说，影响医患沟通的前三位因素依次为：缺乏沟通技巧、工作任务繁重没有时间和精力、服务态度不好；就患者方面责任来说，影响医患沟通的前三位因素依次为：不理解不信任医护人员、对医疗知识不了解、文化层次相对较低。结论  苏州市3家三甲医院医患关系总体较好，医患沟通方面存在一定不足，应当切实增进医患沟通，构建和谐医患关系

Yield enhancement of recombinant α-Amylases in Bacillus amyloliquefaciens by ARTP mutagenesis-screening and medium optimization

α-Amylase is the most extensively applied enzyme in industry. There is an urgent need for improvement on the yield of α-amylases currently. Herein, a strategy which combined Atmospheric and Room Temperature Plasma (ARTP) mutagenesis tool for construction of mutant library of Bacillus amyloliquefaciens with a 24-well plates screening technique was adopted to improve the yield of recombinant Bacillus amyloliquefaciens α-amylases (BAA). A mutant strain named B. amyloliquefaciens ZN mut-7# was obtained, and the activity of BAA produced by this mutant strain was 86.92% higher than that of the original strain. B. amyloliquefaciens ZN mut-7# has an unchanged BAA gene and genetic stability. This successful application proved that ARTP can be applied to the genetically engineering strains that contain recombinant plasmid. Furthermore, response surface methodology offers an achievable and efficient strategy to optimize the composition of medium used to generate BAA in B. amyloliquefaciens ZN mut-7#. A 1.28-fold increase had been obtained compared to the production of non-optimized fermentation medium. This study demonstrates that ARTP mutagenesis and medium optimization are efficient and feasible methods for increasing recombinant enzyme production in the genetically engineering strains

Transcription Expression and Clinical Significance of Dishevelled-3 mRNA and δ-Catenin mRNA in Pleural Effusions from Patients with Lung Cancer

Objective. To evaluate diagnostic utility of Dishevelled-3 (DVL-3) mRNA and δ-catenin mRNA expression in pleural effusions of patients with lung cancer. Methods. DVL-3 mRNA and δ-catenin mRNA levels were assessed by performing RT-PCR on pleural effusion specimens from patients with lung cancer (n = 75) and with lung benign disease (n = 51). Results. The expressions of DVL-3 mRNA and δ-catenin mRNA were significantly higher in malignant than in benign lung disease (P < 0.01) and were obviously higher than cytology in adenocarcinoma (P < 0.01). In single use, DVL-3 mRNA had the highest specificity (94.1%) and PPV (95.7%), whereas δ-catenin mRNA had the highest sensitivity (92.0%) and NPV (88.5%). When combinations of markers were evaluated together, DVL-3 mRNA and δ-catenin mRNA gave a high-diagnostic performance: sensitivity of 100.0%, NPV of 100.0%, and accuracy of 96.0%, respectively. Conclusion. As molecular markers of detecting pleural micrometastasis, DVL-3 mRNA and δ-catenin mRNA are helpful to diagnose the cancer cells in pleural effusions of patients with lung cancer

Identification and Nearly Full-Length Genome Characterization of Novel Porcine Bocaviruses

The genus bocavirus includes bovine parvovirus (BPV), minute virus of canines (MVC), and a group of human bocaviruses (HBoV1-4). Using sequence-independent single primer amplification (SISPA), a novel bocavirus group was discovered with high prevalence (12.59%) in piglet stool samples. Two nearly full-length genome sequences were obtained, which were approximately 5,100 nucleotides in length. Multiple alignments revealed that they share 28.7–56.8% DNA sequence identity with other members of Parvovirinae. Phylogenetic analyses indicated their closest neighbors were members of the genus bocavirus. The new viruses had a putative non-structural NP1 protein, which was unique to bocaviruses. They were provisionally named porcine bocavirus 1 and 2 (PBoV1, PBoV2). PBoV1 and PBoV2 shared 94.2% nucleotide identity in NS1 gene sequence, suggesting that they represented two different bocavirus species. Two additional samples (6V, 7V) were amplified for 2,407 bp and 2,434 bp products, respectively, including a partial NP1 gene and the complete VP1 gene; Phylogenetic analysis indicated that 6Vand 7V grouped with PBoV1 and PBoV2 in the genus of bocavirus, but were in the separate clusters. Like other parvoviruses, PBoV1, PBoV2, 6Vand 7V also contained a putative secretory phospholipase A2 (sPLA2) motif in the VP1 unique region, with a conserved HDXXY motif in the catalytic center. The conserved motif YXGXF of the Ca2+-binding loop of sPLA2 identified in human bocavirus was also found in porcine bocavirus, which differs from the YXGXG motif carried by most other parvoviruses. The observation of PBoV and potentially other new bocavirus genus members may aid in molecular and functional characterization of the genus bocavirus