ZmCom1 Is Required for Both Mitotic and Meiotic Recombination in Maize

CtIP/Ctp1/Sae2/Com1, a highly conserved protein from yeast to higher eukaryotes, is required for DNA double-strand break repair through homologous recombination (HR). In this study, we identified and characterized the COM1 homolog in maize. The ZmCom1 gene is abundantly expressed in reproductive tissues at meiosis stages. In ZmCom1-deficient plants, meiotic chromosomes are constantly entangled as a formation of multivalents and accompanied with chromosome fragmentation at anaphase I. In addition, the formation of telomere bouquet, homologous pairing and synapsis were disturbed. The immunostaining assay showed that the localization of ASY1 and DSY2 was normal, while ZYP1 signals were severely disrupted in Zmcom1 meiocytes, indicating that ZmCom1 is critically required for the proper SC assembly. Moreover, RAD51 signals were almost completely absent in Zmcom1 meiocytes, implying that COM1 is required for RAD51 loading. Surprisingly, in contrast to the Atcom1 and Oscom1 mutants, Zmcom1 mutant plants exhibited a number of vegetative phenotypes under normal growth condition, which may be partly attributed to mitotic aberrations including chromosomal fragmentation and anaphase bridges. Taken together, our results suggest that although the roles of COM1 in HR process seem to be primarily conserved, the COM1 dysfunction can result in the marked dissimilarity in mitotic and meiotic outcomes in maize compared to Arabidopsis and rice. We suggest that this character may be related to the discrete genome context

EXPERIMENTAL STUDY OF THE STABILITY OF NbTi COMPOSITE CONDUCTOR UNDER THE INFLUENCE OF PULSED MAGNETIC FIELD

Nous avons construit un appareillage expérimental destiné à étudier l'influence d'un champ magnétique pulsé sur la stabilité d'un conducteur multifilamentaire composite, torsadé, de NbTi. Plusieurs échantillons ont été mesurés. Nous présentons nos résultats.To study the influence of pulsed magnetic field on the stability of NbTi multifilamentary twisted composite conductor, an experimental device was constructed and several samples have been studied. The corresponding results are presented

IMPROVEMENT ON THE SUPPORT STRUCTURE OF THE SUPERCONDUCTING SADDLE MAGNET SSM-3

Le travail pour l'amélioration de la structure supportante de l'aimant SSM-3 ("adiabatically stable, saddle magnet") est résumé. Cet aimant atteint la performance en échantillon court.The work related to support structure improvement, which allows the adiabatically stable saddle magnet SSM-3 to achieve "short-sample" performance, has been summarized

Quench protection design of a 1.5 T superconducting MRI magnet

A 1.5 T superconducting MRI magnet has been developed in our laboratory. A passive quench protection system is employed to avoid the damage through the quench event. The coils are subdivided into several groups and a heater network is implemented accordingly. With the control volume method, the numerical model of the quench time is introduced. Different design schemes of the heater strip are compared. The simulation results of currents and voltages are illustrated and the temperature rise of the coils and the heaters are discussed

A homogeneous superconducting magnet design using a hybrid optimization algorithm

This paper employs a hybrid optimization algorithm with a combination of linear programming (LP) and nonlinear programming (NLP) to design the highly homogeneous superconducting magnets for magnetic resonance imaging (MRI). The whole work is divided into two stages. The first LP stage provides a global optimal current map with several non-zero current clusters, and the mathematical model for the LP was updated by taking into account the maximum axial and radial magnetic field strength limitations. In the second NLP stage, the non-zero current clusters were discretized into practical solenoids. The superconducting conductor consumption was set as the objective function both in the LP and NLP stages to minimize the construction cost. In addition, the peak-peak homogeneity over the volume of imaging (VOI), the scope of 5 Gauss fringe field, and maximum magnetic field strength within superconducting coils were set as constraints. The detailed design process for a dedicated 3.0 T animal MRI scanner was presented. The homogeneous magnet produces a magnetic field quality of 6.0 ppm peak-peak homogeneity over a 16 cm by 18 cm elliptical VOI, and the 5 Gauss fringe field was limited within a 1.5 m by 2.0 m elliptical region

Image_6_ZmCom1 Is Required for Both Mitotic and Meiotic Recombination in Maize.TIF

<p>CtIP/Ctp1/Sae2/Com1, a highly conserved protein from yeast to higher eukaryotes, is required for DNA double-strand break repair through homologous recombination (HR). In this study, we identified and characterized the COM1 homolog in maize. The ZmCom1 gene is abundantly expressed in reproductive tissues at meiosis stages. In ZmCom1-deficient plants, meiotic chromosomes are constantly entangled as a formation of multivalents and accompanied with chromosome fragmentation at anaphase I. In addition, the formation of telomere bouquet, homologous pairing and synapsis were disturbed. The immunostaining assay showed that the localization of ASY1 and DSY2 was normal, while ZYP1 signals were severely disrupted in Zmcom1 meiocytes, indicating that ZmCom1 is critically required for the proper SC assembly. Moreover, RAD51 signals were almost completely absent in Zmcom1 meiocytes, implying that COM1 is required for RAD51 loading. Surprisingly, in contrast to the Atcom1 and Oscom1 mutants, Zmcom1 mutant plants exhibited a number of vegetative phenotypes under normal growth condition, which may be partly attributed to mitotic aberrations including chromosomal fragmentation and anaphase bridges. Taken together, our results suggest that although the roles of COM1 in HR process seem to be primarily conserved, the COM1 dysfunction can result in the marked dissimilarity in mitotic and meiotic outcomes in maize compared to Arabidopsis and rice. We suggest that this character may be related to the discrete genome context.</p