SIRT3 Protects Rotenone-induced Injury in SH-SY5Y Cells by Promoting Autophagy through the LKB1-AMPK-mTOR Pathway.

SIRT3 is a class III histone deacetylase that modulates energy metabolism, genomic stability and stress resistance. It has been implicated as a potential therapeutic target in a variety of neurodegenerative diseases, including Parkinson's disease (PD). Our previous study demonstrates that SIRT3 had a neuroprotective effect on a rotenone-induced PD cell model, however, the exact mechanism is unknown. In this study, we investigated the underlying mechanism. We established a SIRT3 stable overexpression cell line using lentivirus infection in SH-SY5Y cells. Then, a PD cell model was established using rotenone. Our data demonstrate that overexpression of SIRT3 increased the level of the autophagy markers LC3 II and Beclin 1. After addition of the autophagy inhibitor 3-MA, the protective effect of SIRT3 diminished: the cell viability decreased, while the apoptosis rate increased; α-synuclein accumulation enhanced; ROS production increased; antioxidants levels, including SOD and GSH, decreased; and MMP collapsed. These results reveal that SIRT3 has neuroprotective effects on a PD cell model by up-regulating autophagy. Furthermore, SIRT3 overexpression also promoted LKB1 phosphorylation, followed by activation of AMPK and decreased phosphorylation of mTOR. These results suggest that the LKB1-AMPK-mTOR pathway has a role in induction of autophagy. Together, our findings indicate a novel mechanism by which SIRT3 protects a rotenone-induced PD cell model through the regulation of autophagy, which, in part, is mediated by activation of the LKB1-AMPK-mTOR pathway

Tetra­aqua­tetra­kis­(4,4′-bipyridine dioxide-κO)terbium(III) octa­cyanido­molybdate(V)

In the title compound, [Tb(C10H8N2O2)4(H2O)4][Mo(CN)8], both metal atoms are eight-coordinated. The TbIII atom displays a dodecahedral geometry, while the MoV ion exhibits a distorted square-anti­prismatic geometry. The Tb atoms are located on a special position of site symmetry , whereas the Mo atoms are located on a twofold rotation axis. The cations are linked by O—H⋯O hydrogen bonds

Quorum Sensing System of Ruegeria mobilis Rm01 Controls Lipase and Biofilm Formation

Quorum sensing (QS) promotes in situ extracellular enzyme (EE) activity via the exogenous signal N-acylhomoserine lactone (AHL), which facilitates marine particle degradation, but the species that engage in this regulatory mechanism remain unclear. Here, we obtained AHL-producing and AHL-degrading strains from marine particles. The strain Ruegeria mobilis Rm01 of the Roseobacter group (RBG), which was capable of both AHL producing and degrading, was chosen to represent these strains. We demonstrated that Rm01 possessed a complex QS network comprising AHL-based QS and quorum quenching (QQ) systems and autoinducer-2 (AI-2) perception system. Rm01 was able to respond to multiple exogenous QS signals through the QS network. By applying self-generated AHLs and non-self-generated AHLs and AI-2 QS signal molecules, we modulated biofilm formation and lipase production in Rm01, which reflected the coordination of bacterial metabolism with that of other species via eavesdropping on exogenous QS signals. These results suggest that R. mobilis might be one of the participators that could regulate EE activities by responding to QS signals in marine particles

Rapid Identification of Asteraceae

Plants from Asteraceae family are widely used as herbal medicines and food ingredients, especially in Asian area. Therefore, authentication and quality control of these different Asteraceae plants are important for ensuring consumers’ safety and efficacy. In recent decades, electronic nose (E-nose) has been studied as an alternative approach. In this paper, we aim to develop a novel discriminative model by improving radial basis function artificial neural network (RBF-ANN) classification model. Feature selection algorithms, including principal component analysis (PCA) and BestFirst + CfsSubsetEval (BC), were applied in the improvement of RBF-ANN models. Results illustrate that in the improved RBF-ANN models with lower dimension data classification accuracies (100%) remained the same as in the original model with higher-dimension data. It is the first time to introduce feature selection methods to get valuable information on how to attribute more relevant MOS sensors; namely, in this case, S1, S3, S4, S6, and S7 show better capability to distinguish these Asteraceae plants. This paper also gives insights to further research in this area, for instance, sensor array optimization and performance improvement of classification model

Classification of Traditional Chinese Medicine Syndromes in Patients with Chronic Hepatitis B by SELDI-Based ProteinChip Analysis

Traditional Chinese medicine (TCM) syndrome, also called ZHENG, is the basis concept of TCM theory. It plays an important role in TCM practice. There are excess and deficiency syndromes in TCM syndrome. They are the common syndromes in chronic hepatitis B (CHB) patients. Here we aim to explore serum protein profiles and potential biomarkers for classification of TCM syndromes in CHB patients. 24 healthy controls and two cohorts of CHB patients of excess syndrome (n = 25) or deficiency syndrome (n = 19) were involved in this study. Protein profiles were obtained by surface-enhanced laser desorption ionization time-flight mass spectrometry (SELDI-TOF/MS) and multiple analyses were performed. Based on SELDI ProteinChip data, healthy controls and CHB patients or excess and deficiency syndromes in CHB patients were obviously differentiated by orthogonal partial least square (OPLS) analysis. Two significant serum proteins (m/z 4187 and m/z 5032) for classifying excess and deficiency syndromes were found. Moreover, the area under the receiver operating characteristic (ROC) curve was 0.887 for classifying excess and nonexcess syndrome, and 0.700 for classifying deficiency and nondeficiency syndrome, respectively. Therefore, the present study provided the possibility of TCM syndrome classification in CHB patients using a universally acceptable scientific approach

Bis{tris­[3-(2-pyrid­yl)pyrazole]manganese(II)} dodeca­molybdosilicate hexa­hydrate

The title compound, [Mn(C8H7N3)3]2[SiMo12O40]·6H2O, consists of an [SiMo12O40]4− heteropolyanion, lying on a centre of inversion, and a complex [Mn(C8H7N3)3]4+ cation. The MnII atom of the cation is hexa­coordinated in a distorted octa­hedral geometry by six N atoms from three chelating 3-(2-pyrid­yl)pyrazole ligands. In the heteropolyanion, the four O atoms of the tetra­hedral SiO4 group each half-occupy eight sites due to Si lying on the centre of inversion. N—H⋯O and O—H⋯O hydrogen bonding mediated by the water mol­ecules leads to a consolidation of the structure