Interfacial Interactions between Poly(3-hexylthiophene) and Substrates

Interfacial interactions between poly­(3-hexylthiophene) (P3HT) and substrate surface have been investigated. P3HT nanowhiskers of single molecule thickness were prepared from chloroform solution, and their adsorption on substrates of various surface chemistries was investigated using atomic force microscopy (AFM) and Raman spectroscopy. P3HT monolayer nanowhiskers with edge-on molecular orientation were found to adsorb readily onto a SiO₂ substrate, and the amount of adsorption was significantly higher on a SiO₂ surface modified with a perfluorohexyl monolayer; no P3HT adsorption was observed on a hexyl monolayer. These results suggest that electron-withdrawing groups rather than surface energy govern the interfacial interactions. On a highly oriented pyrolytic graphite (HOPG) surface, P3HT molecules adsorbed in face-on orientation, and edge-on monolayer nanowhiskers were absent on the surface. Raman spectroscopy data revealed strong charge-transfer interactions between face-on P3HT molecules and the HOPG surface

SUR1-associated mechanisms are not involved in ischemic optic neuropathy 1 day post-injury PLOS One Raw Data

<p>The Results of OCT Grading spreadsheet contains the raw data from raters examining OCT images of rat optic nerves at the level of the hyaloid artery. The data shows no significant change in apparent damage at 1 day post-injury with glibenclamide treatment.Optic nerve edema qualitative scoring results with and without PGJ2. Sheet 2 shows raw data for vessel filling calculations.<br></p> <p>The other spreadsheet shows real time PCR data.</p><br

QTL mapping of wheat plant architectural characteristics and their genetic relationship with seven QTLs conferring resistance to sheath blight

<div><p>Sheath blight is one of the most devastating wheat diseases worldwide. Breeding resistant cultivars is the most powerful strategy to defeat the disease. Plant resistance on “disease escape” works through modulation of morphological traits and shows sustainable resistance to disease. Plant architectural traits have been reported to play a significant role in disease response. Therefore, exploring the genetic relationship between plant architecture and disease resistance is of importance to the understanding of plant resistance via “disease escape”. Using an F₉ population of 266 RILs (Recombinant Inbred Lines) derived from the cross of Luke × AQ24788-83, we have generated a linkage map of 631 markers on 21 chromosomes. In this study, we present the QTL identification of fourteen plant architectural characteristics and heading time from two years and analyze their genetic relationships with seven previously published QTLs to sheath blight (QSBs, <i>QSe</i>.<i>cau</i>), including plant height (PH), the space between the flag leaf and penultimate leaf (fdR), heading date (Hd), and other traits. Twelve stable QTLs of the morphological traits were identified with good consistency across five replicates. For the seven previously published QSBs, we found no significant association with plant height. However, some of the QSBs displayed strong associations with plant architectural traits and heading date. Especially, <i>Q</i>_<i>fdR</i>.<i>cau-1AS</i>, <i>Q</i>_<i>Hd</i>.<i>cau-2BS</i>, <i>Q</i>_<i>fdR</i>.<i>cau-5DL</i>, and <i>Q</i>_<i>fdR</i>.<i>cau-6BL</i> were respectively mapped to the same regions as <i>QSe</i>.<i>cau-1AS</i>, <i>QSe</i>.<i>cau-2BS</i>, <i>QSe</i>.<i>cau-5DL</i>, and <i>QSe</i>.<i>cau-6BL</i>. Taken together, we have demonstrated that plant height did not exert a direct influence on the resistance to sheath blight conferred by the seven QSBs and that the plant architecture and heading date did exhibit a tight relationship with the resistance. Therefore, this study provides a novel evidence to help understand sheath blight resistance in wheat. In addition, the linked morphological characteristics and the generated flanking markers will facilitate breeding for resistance to sheath blight in wheat.</p></div

Fluorescent capillary filling of ischemic ON at 1 day post-rAION after glibenclamide or vehicle treatment.

<p>Rats were treated via implanted osmotic pump and i.p. loading dose of either vehicle or glibenclamide. Images show injured ON 1 day post-rAION in maximum intensity projection of confocal microscope z-stacks where vessels were perfused with fluorescent gelatin [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0148855#pone.0148855.ref016" target="_blank">16</a>]. Injury severity ranged from complete loss of perfusion to limited sectorial dropout of perfusion. Previously, we showed a marked improvement in ON perfusion when rAION was treated with a single injection of prostaglandin J₂ [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0148855#pone.0148855.ref016" target="_blank">16</a>]. While we cannot conclude glibenclamide has no effect, this drug does not appear to provide reliable protection against early loss of microvascular perfusion in rAION. For both treatment groups, n = 4. Scale bar = 100 μm.</p

OCT comparison of rAION injured ONH treated with glibenclamide vs. vehicle.

<p>Panels A and B are typical images of rAION at 1 day post-injury treated with vehicle and glibenclamide, respectively. Panel C shows ONH edema measurements across rat ONH in naive rats compared with the edematous diameter with either glibenclamide or vehicle treatment. A normal curve is plotted over each category of data to indicate the mean and variance of the data.</p

LOD Curves of QTLs for Plant Height (PH) on Chromosome 4B.

<p>LOD Curves of QTLs for Plant Height (PH) on Chromosome 4B.</p

Descriptive statistics of EC_a (mS/m) in 2009, 2010 and 2011.

<p>Descriptive statistics of EC_a (mS/m) in 2009, 2010 and 2011.</p

Architecture of the Flag Leaf, the Uppermost Internode, and the Penultimate Leaf of Wheat Plants.

<p>Architecture of the Flag Leaf, the Uppermost Internode, and the Penultimate Leaf of Wheat Plants.</p

LOD Curves of QTLs for Heading Date (Hd) on Chromosome 2B.

<p>The red arrow represents the position of <i>QSe</i>.<i>cau-2BS</i>.</p

LOD Curves of QTLs for the Ratio of Flag Leaf -Penultimate Leaf (fdR) on Chromosome 6B.

<p>The red arrow represents the position of <i>QSe</i>.<i>cau-6BL</i>.</p