A Study on Expression and Tyrosine 705 phosphorylation of STAT3 in Focal Cerebral Ischemia-Reperfusion Rat Model and its Role in Neuronal Apoptosis

Purpose: To investigate the expression and tyrosine 705 phosphorylation of STAT3 in focal cerebral ischemia-reperfusion rat model and its role in neuronal apoptosis.Methods: Ischemia-reperfusion model was established by thread-occluded method. Tetrazolium red (TTC), H/E and Nissl staining were used to evaluate whether ischemia-reperfusion model was successfully established. TUNEL staining and immunohistochemistry were employed to monitor apoptosis-positive nerve cells as well as STAT3-, p-Tyr705-STAT3-, Bcl-2- and Fas-positive cells in ischemic penumbra (IP) and ischemic core (IC).Results: The results of TTC, HE and Nissl staining indicated that the ischemia reperfusion model was successfully established. After 3 h, ischemia followed by different reperfusion times, the STAT3-, p- Tyr705-STAT3-, Fas- and Bcl-2 positive cells counts and the apoptosis-positive nerve cells count were significantly (p < 0.05 or 0.01) increased to 27.20, 29.20, 15.90, 18.50, and 202.00 in IP and 19.50, 21.20, 12.50, 12.40, and 97.80 in IC, compared with the sham-operated group. As reperfusion times increased, cell counts did not decrease significantly relative to control group. Correlation analysis indicate that there was significant (p < 0.01) positive correlations among STAT3-, p-Tyr705 STAT3-, Fas- and Bcl-2-positive cells counts on the one hand, and apoptosis positive nerve cells count in IP and IC, on the other hand.Conclusion: Regulating expression and tyrosine 705 phosphorylation of STAT3 may be a new and effective strategy for treating cerebral infarction.Keywords: Ischemia-reperfusion, Neuronal apoptosis, STAT3, Phosphorylation, Cerebral infarctio

Design of Pulse Forming Networks Triggered by High-Power Hydrogen Thyratron

Hydrogen thyratron is a switching device invented based on the phenomenon of gas discharge, and it is widely used in the field of high-power pulse technology. The design of Pulse Forming Network (PFN) triggered by hydrogen thyratron aims to control the switch of subsequent circuit, and shorten the gate-cathode voltage and conduction delay time by increasing the rise rate of the trigger voltage. However, in the currently adopted series resonance network design schemes, usually the value of inductance is very large, which can easily lead to the decline in the electromagnetic compatibility performance; moreover, the large distribution of network component parameters will greatly increase the fabrication difficulties. In view of the features of high-power hydrogen thyratron and the design requirements of PFN, this paper adopted the series resonance network design scheme to devise network series and parameters of the PFN and analyze the shortcomings of the series resonance network design scheme; then, it used the anti-resonance network to design a three-stage transform algorithm model, so as to achieve the purpose of reducing the inductance of the PFN and the difficulty of capacitance model selection in engineering practice. At last, simulation results verified the correctness and feasibility of the designed three-stage transform algorithm model, providing evidences for the pulse network projects of hydrogen thyratron and other high-power equipment in terms of implementation paths, methods, and algorithm models

(Acetone-2κO){μ-6,6′-dimeth­oxy-2,2′-[propane-1,2-diylbis(nitrilo­methyl­idyne)]diphenolato-κ81:2O 6,O 1,O 1′,O 6′:O 1,N,N′,O 1′}tris(nitrato-1κ2 O,O′)copper(II)terbium(III)

In the title heteronuclear complex, [CuTb(C19H20N2O4)(NO3)3(CH3COCH3)], the CuII ion is five-coordinated by two O and two N atoms from the 6,6′-dimeth­oxy-2,2′-[propane-1,2-diylbis(nitrilo­methyl­idyne)]diphenolate ligand (L) and an O atom from the acetone mol­ecule in a square-pyramidal geometry. The TbIII ion is ten-coordinated by six O atoms from three chelating nitrate ligands and four O atoms from the L ligand. In L, the CH2–CH–CH3 fragment is disordered over two conformations, with refined occupancies of 0.725 (11) and 0.275 (11)

Production of Spin-Semiconducting Zigzag Graphene Nanoribbons by Constructing Asymmetric Notch on Graphene Edges

The electronic and magnetic properties of zigzag graphene nanoribbons with asymmetric notches along their edges are investigated by first principle density functional theory calculations. It is found that the electronic and magnetic properties of the asymmetrically-notched graphene nanoribbons are closely related with the depth of notches, but weekly dependent on the length of notches. As the relative depth of notch increases, the energy level of spin-up and spin-down becomes greatly shifted, associated with the gradual increase of magnetic momentum. The asymmetric band shift allows the asymmetrically notched graphene nanoribbons to be a spintronic semiconductor, through which an N- or P-type spin-semiconductor can be obtained by doping B or N atoms

Effects of sea-buckthorn leaves on performance and serum metabolic profiles in Altay lambs

In this study, the effects of sea-buckthorn (Hippophae rhamnoides L.) leaves (SL) on the biochemical parameters and metabolomic profiling in Altay lamb (Ovis aries) were observed. Sixty six-month-old male Altay lambs (body weight 28.0 ± 3.5 kg) were randomly assigned to four groups (n=15). The experimental groups were named as CON, 2.5%, 5.0% and 7.5%. The group CON, contained animals fed with a basal diet. Animals of the other groups were fed a treatment diet consisting of 2.5% (Group 2.5%), 5.0% (Group 5.0%) and 7.5% (Group 7.5%) SL. The experimental period lasted 56 days. The results showed that the average daily gain (ADG) and average daily feed intake (ADFI) increased with the increase in the levels of dietary SL. Dietary SL showed a direct relationship with total protein (TP), albumin, globulin and total cholesterol (TC) content of the experimental animals. However, an indirect relationship was observed between dietary SL and the concentration of urea nitrogen (UN). The concentrations of glucose, high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) showed quadratic change. Additional changes occurred in the endogenous metabolites involving multiple pathways. The pathways were tricarboxylic acid (TCA) cycle, the metabolism of protein and amino acid and the metabolism of fatty acid and steroid. The changes in metabolites primarily revealed an increase in amino acids and carbohydrates and a decrease in lipid metabolites. These findings provide a comprehensive insight into the effects of the metabolic aspects of sea-buckthorn leaves on Altay lambs. In addition, the present research results provide a better understanding to the development and utilization of sea-buckthorn as a healthy additive for small ruminant production.Keywords: Hippophae rhamnoides L., lamb, metabolomics, growth performance, serum parameter