Medium optimization for endochitinase production by recombinant Pichia pastoris ZJGSU02 using response surface methodology

Plackett-Burman design and response surface methodology were employed to optimize the medium components for endochitinase production by Pichia pastoris. A Plackett- Burman design of seven factors with 12 runs was applied to evaluate the effects of different medium components. Yeast extract, oleic acid and Tween-80 were found to have significant influence on endochitinase production. The optimal concentrations of three factors were investigated by the response surface methodology using Box- Behnken design. The optimal medium components obtained for achieving the maximum activity of the endochitinase were as follows: Yeast extract 24.36 g/l, tryptone 20 g/l, YNB 5.0 g/l, potassium phosphate 100 mM, methanol 5 ml/l, oleic acid 1.758 ml/l, Tween-80 6.2 ml/l, Pichia trace metals (PTM1) 4.0 ml/ l and biotin 4.00 × 10-4 g/l. Under these conditions, endochitinase activity was up to 88.26 μ/ml, which was about 1.14-fold higher than using the original medium (77.62 μ/ml). This work will be very helpful for large-scale production of endochitinase for future industrial application.Keywords: Recombinant endochitinase, Plackett-Burman design, response surface methodology, fermentatio

A New Method for Fast Computation of Moments Based on 8-neighbor Chain CodeApplied to 2-D Objects Recognition

2D moment invariants have been successfully applied in pattern recognition tasks. The main difficulty of using moment invariants is the computational burden. To improve the algorithm of moments computation through an iterative method, an approach for fast computation of moments based on the 8-neighbor chain code is proposed in this paper. Then artificial neural networks are applied for 2D shape recognition with moment invariants. Compared with the method of polygonal approximation, this approach shows higher accuracy in shape representation and faster recognition speed in experiment

A Practical Case Study of the Interactive TV Service as a Time-Critical Product

In this paper, we conducted a case study of time-critical goods - NG goods. We expected the study to integrate the field of information management and the TV broadcasting field, thereby creating a new wave of potential for the information management field after e-commerce. We suggest two perspectives germane to industry development: the development of the whole industry, and, the operation of the individual companie

Multiparty quantum secret splitting and quantum state sharing

A protocol for multiparty quantum secret splitting is proposed with an ordered $N$ EPR pairs and Bell state measurements. It is secure and has the high intrinsic efficiency and source capacity as almost all the instances are useful and each EPR pair carries two bits of message securely. Moreover, we modify it for multiparty quantum state sharing of an arbitrary $m$-particle entangled state based on quantum teleportation with only Bell state measurements and local unitary operations which make this protocol more convenient in a practical application than others.Comment: 7 pages, 1 figure. The revision of the manuscript appeared in PLA. Some procedures for detecting cheat have been added. Then the security loophole in the original manuscript has been eliminate