Heat shock protein 27 is a potential indicator for response to YangZheng XiaoJi and chemotherapy agents in cancer cells

Heat shock protein 27 (HSP27) is a member of the heat shock protein family which has been linked to tumour progression and, most interestingly, to chemotherapy resistance in cancer patients. The present study examined the potential interplay between HSP27 and YangZheng XiaoJi, a traditional Chinese medicine used in cancer treatment. A range of cell lines from different tumour types including pancreatic, lung, gastric, colorectal, breast, prostate and ovarian cancer (both wild-type and resistant) were used. Levels and activation of HSP27 and its potential associated signalling pathways were evaluated by protein array and western blotting. Knockdown of HSP27 in cancer cells was achieved using siRNA. Localisation and co-localisation of HSP27 and other proteins were carried out by immunofluorescence. Cell growth and migration were evaluated in their response to a range of chemotherapeutic agents. The present study first identified, by way of protein array, that YangZheng XiaoJi was able to inhibit the phosphorylation of HSP27 protein in cancer cells. We further demonstrated that HSP27, which is co-localised with caspase-9, can be blocked from localising in focal adhesions and co-localising with caspase-9 by YangZheng XiaoJi. The study also demonstrated that YangZheng XiaoJi was able to sensitise cancer cells including those cells that were resistant to chemotherapy, to chemotherapeutic agents. Finally, knocking down HSP27 markedly reduced the migration of cancer cells and increased the sensitivity of cancer cells to the inhibitory effect on cellular migration by YangZheng XiaoJi. YangZheng XiaoJi can act as an agent in first sensitising cancer cells to chemotherapy and secondly to overcome, to some degree, chemoresistance when used in an appropriate fashion in patients who have active HSP2

Effect of fermented heat-treated rice bran on performance and possible role of intestinal microbiota in laying hens

Rice bran is a high-quality and renewable livestock feed material rich in nutrients and bioactive substances. To investigate the effects of dietary supplementation with fermented heat-treated rice bran on the performance, apparent digestibility of nutrients, cecal microbiota and metabolites in laying hens, a total of 128 18-week-old Hy-Line brown layers were randomly assigned to four treatment groups: 2.5% HRB (basal diet contained 2.5% heat-treated rice bran), 5.0% HRB (5.0% heat-treated rice bran), 2.5% FHRB (2.5% fermented heat-treated rice bran), 5.0% FHRB (5.0% fermented heat-treated rice bran). Results showed that FHRB supplementation significantly increased the average daily feed intake (ADFI) during 25–28 weeks, and improved apparent digestibility of dry matter (DM), crude protein (CP), ether extract (EE) and crude fiber (CF) in laying hens. Moreover, feeding 5.0% of HRB and FHRB resulted higher egg production (EP) and average egg weight (AEW) during the feeding period, and decreased the feed conversion ratio (FCR) during 21 to 28 weeks. The alpha and beta diversity indices indicated that FHRB altered the cecal microbiota. In particular, dietary supplementation with FHRB significantly increased the relative abundances of Lachnospira and Clostridium. Compared with the 2.5% level of supplementation, supplementing 5.0% HRB and 5.0% FHRB increased the relative abundances of Firmicutes, Ruminococcus and Peptococcus, and lowered the relative abundance of Actinobacteria. Furthermore, dietary FHRB supplementation significantly increased the concentration of short-chain fatty acids in cecum and changed the overall metabolome. The results of correlation analysis showed a close interaction between cecal microbiota, metabolites and apparent digestibility of nutrients. Taken together, we revealed that FHRB supplementation can induce characteristic structural and metabolic changes in the cecal microbiome, which could potentially promote nutrient digestion and absorption, and improve the production performance of laying hens

Construction of a high-density genetic map for faba bean (Vicia faba L.) and quantitative trait loci mapping of seed-related traits

Faba bean (Vicia faba L.) is a valuable legume crop and data on its seed-related traits is required for yield and quality improvements. However, basic research on faba bean is lagging compared to that of other major crops. In this study, an F2 faba bean population, including 121 plants derived from the cross WY7×TCX7, was genotyped using the Faba_bean_130 K targeted next-generation sequencing genotyping platform. The data were used to construct the first ultra-dense faba bean genetic map consisting of 12,023 single nucleotide polymorphisms markers covering 1,182.65 cM with an average distance of 0.098 cM. The map consisted of 6 linkage groups, which is consistent with the 6 faba bean chromosome pairs. A total of 65 quantitative trait loci (QTL) for seed-related traits were identified (3 for 100-seed weight, 28 for seed shape, 12 for seed coat color, and 22 for nutritional quality). Furthermore, 333 candidate genes that are likely to participate in the regulation of seed-related traits were also identified. Our research findings can provide a basis for future faba bean marker-assisted breeding and be helpful to further modify and improve the reference genome

Fast Response Isopropanol Sensing Properties with Sintered BiFeO3 Nanocrystals

BiFeO3 nanocrystals were applied as the sensing material to isopropanol. The isopropanol sensor based on BiFeO3 nanocrystals shows excellent gas-sensing properties at the optimum working temperature of 240 °C. The sensitivity of as-prepared sensor to 100 ppm isopropanol is 31 and its response and recovery time is as fast as 6 and 17 s. The logarithmic curves of the sensitivity and concentration of BiFeO3 sensors are a very good linear in the low detection range of 2–100 ppm. In addition, the gas sensing mechanism is also discussed. The results suggest that the BiFeO3 nanomaterial can be potentially applied in isopropanol gas detection

Panxian Dadong, South China: Establishing a Record of Middle Pleistocene Climatic Changes

Broad-based reconstructions of the Middle Pleistocene Asian environment are valuable sources of information that can augment our understanding of prehistoric human adaptations and expansion into East Asia. The sediments, speleothems, and geochronology of Panxian Dadong Cave serve as an example of the possible integration of this broader paleoenvironmental information with more fine-grained archaeological data. The current U-series and ESR dating results for Dadong suggest that the early human activity in the cave began at least 260 kya and continued until around 142 kya. This period correlates with Oxygen Isotope Stages 7 through 6. The lower part of the breccia (Layer 2) contains very strongly weathered dark deposits, suggesting a relatively warm climatic period from 260-180 kya that corresponds to OIS 7, followed by a cooler phase with less speleothem formation corresponding to OIS 6. The Middle Pleistocene stratigraphic sequence in the Dadong cave deposits documents fluctuating and rapid changes in temperature and humidity that are also detected in general Asian, as well as South China, paleoclimatic studies based on diverse data ranging from microstratigraphic and geochemical sediment analyses to mollusk species representation. KEYWORDS: Middle Pleistocene, Asian paleoenvironment, stratigraphy, speleothem, Panxian Dadong, China

Virtual Flux Voltage-Oriented Vector Control Method of Wide Frequency Active Rectifiers Based on Dual Low-Pass Filter

This article presents a non-AC-side voltage sensor control method applied to More Electric Aircraft rectifiers. The control strategy can operate properly over a wide range of frequencies. This strategy calculates the AC supply frequency through an instantaneous phase-locked loop and feeds it back to a dual low-pass filter. The reconstructed rectifier-side voltage is filtered using two low-pass filters with different scale factors. Then, the values of the two filter outputs are subtracted and the effect of the DC bias due to the initial value of the integration is eliminated. The subtracted value is amplitude-phase compensated to calculate the virtual flux value. The phase angle can then be calculated from the virtual flux value. This phase angle is used for the implementation of the voltage-oriented vector control and as an input to the instantaneous phase-locked loop. Simulation and experimental results show that the use of dual low-pass filters under different frequency conditions improves the speed and accuracy of virtual flux estimation and eliminates DC-side bias errors

Enhanced and tunable Goos-Hänchen shift of reflected light due to Tamm surface plasmons with Dirac semimetals

In this paper, enhanced and tunable Goos-Hänchen (GH) shift of reflected light beam at mid-infrared band can be theoretically achieved by using a multilayer structure where a bulk Dirac semimetal (BDS) layer is coated on a distributed bragg reflector (DBR) separated by a spacer layer. This enhanced GH phenomenon results from the local field enhancement owing to the excitation of Tamm surface plasmons at the interface between BDS layer and spacer layer. Numerical calculation shows that the GH shift can be switched from negative to positive by harnessing the bulk conductivity properties of BDS, and can also be actively tuned through the Fermi energy of BDS or by controlling the structural parameters. These enhanced and tunable GH shifts are promising for fabricating BDS-based infrared shift devices and other applications in nanophotonics

Optimal Design and Verification of Informal Learning Spaces (ILS) in Chinese Universities Based on Visual Perception Analysis

As the focus on higher education in China gradually shifts from rapid development to an emphasis on quality, the need for campus environments to become facilitators of education has gained increasing attention. The accelerated development of information technology has also led to tremendous changes in both teaching and learning methods, with informal learning taking on an increasingly important role. Furthermore, the development of human sensing technology, especially visual perception technology, has brought in new opportunities for the research and optimization of informal learning spaces (ILSs) in universities. This paper focuses on the ILS in Chinese universities by exploring optimal design approaches based on visual perception analysis. Through research and field investigation, this paper proposes revised theoretical research of classifications and spatial elements of ILS in universities more applicable to the architectural study of space. This paper also explores practical optimal design methods with two case studies and makes experiments with wearable eye trackers to study the users’ perception in these spaces before and after optimization. The optimal design is made from the aspects of physical space, facilities, and environment. Visual perception experiments and quantitative analysis were used to obtain a higher level of experimental accuracy than the previous studies and thus to study the real feeling of users in spaces. By these means, the effect of the optimized design was verified and the relation between users’ perceptions and the spatial environments was explored for further improvements to optimal design methods. This article can provide theoretical and practical references for campus space optimization research and design, especially for ILS on university campuses

Virtual Flux Voltage-Oriented Vector Control Method of Wide Frequency Active Rectifiers Based on Dual Low-Pass Filter

This article presents a non-AC-side voltage sensor control method applied to More Electric Aircraft rectifiers. The control strategy can operate properly over a wide range of frequencies. This strategy calculates the AC supply frequency through an instantaneous phase-locked loop and feeds it back to a dual low-pass filter. The reconstructed rectifier-side voltage is filtered using two low-pass filters with different scale factors. Then, the values of the two filter outputs are subtracted and the effect of the DC bias due to the initial value of the integration is eliminated. The subtracted value is amplitude-phase compensated to calculate the virtual flux value. The phase angle can then be calculated from the virtual flux value. This phase angle is used for the implementation of the voltage-oriented vector control and as an input to the instantaneous phase-locked loop. Simulation and experimental results show that the use of dual low-pass filters under different frequency conditions improves the speed and accuracy of virtual flux estimation and eliminates DC-side bias errors