Cosmic star formation history revealed by the AKARI, & Spatially-resolved spectroscopy of an E+A (Post-starburst) system

We reveal cosmic star-formation history obscured by dust using deep infrared observation with the AKARI. A continuous filter coverage in the mid-IR wavelength (2.4, 3.2, 4.1, 7, 9, 11, 15, 18, and 24um) by the AKARI satellite allows us to estimate restframe 8um and 12um luminosities without using a large extrapolation based on a SED fit, which was the largest uncertainty in previous work. We found that restframe 8um (0.38<z<2.2), 12um (0.15<z<1.16), and total infrared (TIR) luminosity functions (LFs) (0.2<z<1.6) constructed from the AKARI NEP deep data, show a continuous and strong evolution toward higher redshift. In terms of cosmic infrared luminosity density (Omega_IR), which was obtained by integrating analytic fits to the LFs, we found a good agreement with previous work at z<1.2, with Omega_IR propto (1+z)^4.4+-1.0. When we separate contributions to Omega_IR by LIRGs and ULIRGs, we found more IR luminous sources are increasingly more important at higher redshift. We found that the ULIRG (LIRG) contribution increases by a factor of 10 (1.8) from z=0.35 to z=1.4.Comment: To appear in "Hunting for the Dark: The Hidden Side of Galaxy Formation", Malta, 19-23 Oct. 2009, eds. V.P. Debattista & C.C. Popescu, AIP Conf. Se

Far-infrared all sky diffuse mapping with AKARI

We discuss the capability of AKARI in recovering diffuse far-infrared emission, and examine the achieved reliability. Critical issues in making images of diffuse emission are the transient response and long-term stability of the far-infrared detectors. Quantitative evaluation of these characteristics are the key to achieving sensitivity comparable to or better than that for point sources (< 20 -- 95 MJy sr-1). We describe current activity and progress toward the production of high quality images of the diffuse far-infrared emission using the AKARI all-sky survey data.Comment: 4 pages, 8 figures, to appear in the Proceedings of the Conference "AKARI, a light to illuminate the misty Universe", Fukutake Hall, The University of Tokyo, Japan, 16-19 February 200

AKARI Far-Infrared All Sky Survey

We demonstrate the capability of AKARI for mapping diffuse far-infrared emission and achieved reliability of all-sky diffuse map. We have conducted an all-sky survey for more than 94 % of the whole sky during cold phase of AKARI observation in 2006 Feb. -- 2007 Aug. The survey in far-infrared waveband covers 50 um -- 180 um with four bands centered at 65 um, 90 um, 140 um, and 160 um and spatial resolution of 3000 -- 4000 (FWHM).This survey has allowed us to make a revolutionary improvement compared to the IRAS survey that was conducted in 1983 in both spatial resolution and sensitivity after more than a quarter of a century. Additionally, it will provide us the first all-sky survey data with high-spatial resolution beyond 100 um. Considering its extreme importance of the AKARI far-infrared diffuse emission map, we are now investigating carefully the quality of the data for possible release of the archival data. Critical subjects in making image of diffuse emission from detected signal are the transient response and long-term stability of the far-infrared detectors. Quantitative evaluation of these characteristics is the key to achieve sensitivity comparable to or better than that for point sources (< 20 -- 95 [MJy/sr]). We describe current activities and progress that are focused on making high quality all-sky survey images of the diffuse far-infrared emission.Comment: To appear in Proc. Workshop "The Space Infrared Telescope for Cosmology & Astrophysics: Revealing the Origins of Planets and Galaxies". Eds. A.M. Heras, B. Swinyard, K. Isaak, and J.R. Goicoeche

Subzero Nonfreezing Hypothermia with Insect Antifreeze Protein Dramatically Improves Survival Rate of Mammalian Cells

Cells for therapeutic use are often preserved at +4 &deg;C, and the storage period is generally limited to 2&ndash;3 days. Here, we report that the survival rate (%) of mammalian cells is improved to 10&ndash;20 days when they are preserved with a subzero supercooled solution containing the antifreeze protein (AFP), for which an ability to stabilize both supercooled water and cell membrane integrity has been postulated. We chose adherent rat insulinoma (RIN-5F) cells as the preservation target, which were immersed into &minus;5 &deg;C-, &minus;2 &deg;C-, or +4 &deg;C-chilled &ldquo;unfrozen&rdquo; solution of Euro-Collins or University of Washington (UW) containing the AFP sample obtained from insect or fish. Our results show that the survival rate of the cells preserved with the solution containing insect AFP was always higher than that of the fish AFP solution. A combination of the &minus;5 &deg;C-supercooling and insect AFP gave the best preservation result, namely, UW solution containing insect AFP kept 53% of the cells alive, even after 20 days of preservation at &minus;5 &deg;C. The insect AFP locates highly organized ice-like waters on its molecular surface. Such waters may bind to semiclathrate waters constructing both embryonic ice crystals and a membrane&ndash;water interface in the supercooled solution, thereby protecting the cells from damage due to chilling

Subzero Nonfreezing Hypothermia with Insect Antifreeze Protein Dramatically Improves Survival Rate of Mammalian Cells

Cells for therapeutic use are often preserved at +4 °C, and the storage period is generally limited to 2–3 days. Here, we report that the survival rate (%) of mammalian cells is improved to 10–20 days when they are preserved with a subzero supercooled solution containing the antifreeze protein (AFP), for which an ability to stabilize both supercooled water and cell membrane integrity has been postulated. We chose adherent rat insulinoma (RIN-5F) cells as the preservation target, which were immersed into −5 °C-, −2 °C-, or +4 °C-chilled “unfrozen” solution of Euro-Collins or University of Washington (UW) containing the AFP sample obtained from insect or fish. Our results show that the survival rate of the cells preserved with the solution containing insect AFP was always higher than that of the fish AFP solution. A combination of the −5 °C-supercooling and insect AFP gave the best preservation result, namely, UW solution containing insect AFP kept 53% of the cells alive, even after 20 days of preservation at −5 °C. The insect AFP locates highly organized ice-like waters on its molecular surface. Such waters may bind to semiclathrate waters constructing both embryonic ice crystals and a membrane–water interface in the supercooled solution, thereby protecting the cells from damage due to chilling

Discovery of Hyperactive Antifreeze Protein from Phylogenetically Distant Beetles Questions Its Evolutionary Origin

Beetle hyperactive antifreeze protein (AFP) has a unique ability to maintain a supercooling state of its body fluids, however, less is known about its origination. Here, we found that a popular stag beetle Dorcus hopei binodulosus (Dhb) synthesizes at least 6 isoforms of hyperactive AFP (DhbAFP). Cold-acclimated Dhb larvae tolerated −5 °C chilled storage for 24 h and fully recovered after warming, suggesting that DhbAFP facilitates overwintering of this beetle. A DhbAFP isoform (~10 kDa) appeared to consist of 6−8 tandem repeats of a 12-residue consensus sequence (TCTxSxNCxxAx), which exhibited 3 °C of high freezing point depression and the ability of binding to an entire surface of a single ice crystal. Significantly, these properties as well as DNA sequences including the untranslated region, signal peptide region, and an AFP-encoding region of Dhb are highly similar to those identified for a known hyperactive AFP (TmAFP) from the beetle Tenebrio molitor (Tm). Progenitor of Dhb and Tm was branched off approximately 300 million years ago, so no known evolution mechanism hardly explains the retainment of the DNA sequence for such a lo­ng divergence period. Existence of unrevealed gene transfer mechanism will be hypothesized between these two phylogenetically distant beetles to acquire this type of hyperactive AFP