DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis

Lim15/Dmc1 is a meiosis specific RecA-like protein. Here we propose its participation in meiotic chromosome pairing-related events along with DNA topoisomerase II. Analysis of protein–protein interactions using in vitro binding assays provided evidence that Coprinus cinereus DNA topoisomerase II (CcTopII) specifically interacts with C.cinereus Lim15/Dmc1 (CcLim15). Co-immunoprecipitation experiments also indicated that the CcLim15 protein interacts with CcTopII in vivo. Furthermore, a significant proportion of CcLim15 and CcTopII could be shown to co-localize on chromosomes from the leptotene to the zygotene stage. Interestingly, CcLim15 can potently activate the relaxation/catenation activity of CcTopII in vitro, and CcTopII suppresses CcLim15-dependent strand transfer activity. On the other hand, while enhancement of CcLim15's DNA-dependent ATPase activity by CcTopII was found in vitro, the same enzyme activity of CcTopII was inhibited by adding CcLim15. The interaction of CcLim15 and CcTopII may facilitate pairing of homologous chromosomes

Interoperable Private Attribution: A Distributed Attribution and Aggregation Protocol

Measuring people’s interactions that span multiple websites can provide unique insight that enables better products and improves people’s experiences, but directly observing people’s individual journeys creates privacy risks that conflict with the newly emerging privacy model for the web. We propose a protocol that uses the combination of multi-party computation and differential privacy that enables the processing of peoples’ data such that only aggregate measurements are revealed, strictly limiting the information leakage about individual people. Our primary application of this protocol is measuring, in aggregate, the effectiveness of digital advertising without enabling cross-site tracking of individuals. In this paper we formalize our protocol, Interoperable Private Attribution (IPA), and analyze its security. IPA is proposed in the W3C’s Private Advertising Technology Community Group (PATCG) [8]. We have implemented our protocol in the malicious honest majority MPC setting for three parties where network costs dominate compute costs. For processing a query with 1M records it uses around 18GB of network which at \$0.08 per GB leads to a network cost of \$1.44

An observation of the walking behavior of Podosphaeraster toyoshiomaruae collected from the bank Oshima-shinsone, Kagoshima Prefecture, Japan

トヨシオマリヒトデPodosphaeraster toyoshiomaruae Fujita and Rowe, 2002は体がほぼ球形で，腕が発達しないという特殊な形態を持つ小型ヒトデ類である。鹿児島県奄美大島北西部に位置する大島新曽根水深100-200 m 程度の堆に生息している。この堆はROV で観察するとカイメン類，八放サンゴ類などで覆われている。2017年5月21日に本種の生きた個体が大島新曽根で採集され，管足を伸ばした状態や歩行が観察されたのでその行動を記載した。管足を体内にしまった状態とは異なり，体がやや口・肛門軸方向に扁平になり，約1.65 cm/min の速度で歩行した。通常の腕の発達したヒトデ類の歩行速度と比較すると相対的に著しく遅い。管足には少なくとも2種類が認められ，歩行用と感覚用と考えられる。The podosphaerastrid asteroid Podosphaeraster toyoshiomaruae Fujita and Rowe, 2002 is small-sized, nearly spherical in shape. The species exclusively inhabits on the bank Oshima-shinsone at depths of 100 to 200 m, northwest of Amami-Oshima Island, Kagoshima Prefecture, Japan, where the bottom is entirely covered with sponges, octocorals, and hyrozoan polyps with patches of sandy bottom. The bottom conditions were clearly observed with a ROV on September 29, 2009. A living specimen was collected from this locality on May 21, 2017. During locomotion by tube feet, the body was depressed dorso-ventrally in contrast with the spherical body shape with tube feet unexpanded. The locomotion rate of the specimen was about 1.65 cm/min. Two types of tube feet were identified. Presumably one is for locomotion with a sucker terminally, while the other for sensing.本研究の一部は日本学術振興会科学研究費（基盤研究C，No.16K07825，代表 大塚攻），国立科学博物館総合研究「黒潮に注目した地史・生物史・人類史」によって行われた

Artificial intelligence for diagnosis and Gleason grading of prostate cancer: The PANDA challenge

Through a community-driven competition, the PANDA challenge provides a curated diverse dataset and a catalog of models for prostate cancer pathology, and represents a blueprint for evaluating AI algorithms in digital pathology. Artificial intelligence (AI) has shown promise for diagnosing prostate cancer in biopsies. However, results have been limited to individual studies, lacking validation in multinational settings. Competitions have been shown to be accelerators for medical imaging innovations, but their impact is hindered by lack of reproducibility and independent validation. With this in mind, we organized the PANDA challenge-the largest histopathology competition to date, joined by 1,290 developers-to catalyze development of reproducible AI algorithms for Gleason grading using 10,616 digitized prostate biopsies. We validated that a diverse set of submitted algorithms reached pathologist-level performance on independent cross-continental cohorts, fully blinded to the algorithm developers. On United States and European external validation sets, the algorithms achieved agreements of 0.862 (quadratically weighted kappa, 95% confidence interval (CI), 0.840-0.884) and 0.868 (95% CI, 0.835-0.900) with expert uropathologists. Successful generalization across different patient populations, laboratories and reference standards, achieved by a variety of algorithmic approaches, warrants evaluating AI-based Gleason grading in prospective clinical trials.KWF Kankerbestrijding ; Netherlands Organization for Scientific Research (NWO) ; Swedish Research Council European Commission ; Swedish Cancer Society ; Swedish eScience Research Center ; Ake Wiberg Foundation ; Prostatacancerforbundet ; Academy of Finland ; Cancer Foundation Finland ; Google Incorporated ; MICCAI board challenge working group ; Verily Life Sciences ; EIT Health ; Karolinska Institutet ; MICCAI 2020 satellite event team ; ERAPerMe

A deep learning approach to photo–identification demonstrates high performance on two dozen cetacean species

We thank the countless individuals who collected and/or processed the nearly 85,000 images used in this study and those who assisted, particularly those who sorted these images from the millions that did not end up in the catalogues. Additionally, we thank the other Kaggle competitors who helped develop the ideas, models and data used here, particularly those who released their datasets to the public. The graduate assistantship for Philip T. Patton was funded by the NOAA Fisheries QUEST Fellowship. This paper represents HIMB and SOEST contribution numbers 1932 and 11679, respectively. The technical support and advanced computing resources from University of Hawaii Information Technology Services—Cyberinfrastructure, funded in part by the National Science Foundation CC* awards # 2201428 and # 2232862 are gratefully acknowledged. Every photo–identification image was collected under permits according to relevant national guidelines, regulation and legislation.Peer reviewedPublisher PD

Recent Results from LHD Experiment with Emphasis on Relation to Theory from Experimentalist’s View

he Large Helical Device (LHD) has been extending an operational regime of net-current free plasmas towardsthe fusion relevant condition with taking advantage of a net current-free heliotron concept and employing a superconducting coil system. Heating capability has exceeded 10 MW and the central ion and electron temperatureshave reached 7 and 10 keV, respectively. The maximum value of β and pulse length have been extended to 3.2% and 150 s, respectively. Many encouraging physical findings have been obtained. Topics from recent experiments, which should be emphasized from the aspect of theoretical approaches, are reviewed. Those are (1) Prominent features in the inward shifted configuration, i.e., mitigation of an ideal interchange mode in the configuration with magnetic hill, and confinement improvement due to suppression of both anomalous and neoclassical transport, (2) Demonstration ofbifurcation of radial electric field and associated formation of an internal transport barrier, and (3) Dynamics of magnetic islands and clarification of the role of separatrix