Potent Vasodilatory Effect of Fasudil on Radial Artery Graft in Coronary Artery Bypass Operations

Background: The radial artery (RA) is a useful conduit for coronary artery bypass grafting (CABG) but is susceptible to vasospasm during harvesting. We evaluated the usefulness of fasudil, a Rho kinase inhibitor, in dilating the RA graft and increasing graft free flow (GFF) compared with the conventional graft-dilating agents papaverine and verapamil-nitroglycerin (VG). Methods: Between June 2012 and January 2013, 45 patients with ischemic heart disease who underwent isolated CABG using the RA were enrolled and randomly assigned to fasudil (n = 15), papaverine (n = 15), or VG (n = 15). Fasudil (2.67 mmol/L), papaverine (1.0 mmol/L) mixed with heparinized blood, or VG (30 μmol/L each of verapamil and nitroglycerin) was injected intraluminally into the RA graft after harvesting. Main outcome measures were RA GFF, hemodynamic changes, and histopathologic examination of the RA. Results: In the fasudil group, GFF increased significantly (p < 0.001) from 36.8 ± 20.4 at baseline to 148.0 ± 88.3 mL/min after injection. GFF increased significantly (p < 0.001) from 36.0 ± 19.0 to 72.3 ± 36.7 mL/min in the papaverine group and increased significantly (p < 0.001) from 39.5 ± 23.3 to 64.3 ± 29.9 mL/min in the VG group. The GFF was significantly higher (p = 0.001) in fasudil-treated RA than in papaverine- or VG-treated RA. Histopathologically, RA graft diameter was markedly increased after fasudil injection, and the structure of the multiple elastic lamellae was intact. Blood pressure did not change significantly after drug injection in all groups. Conclusions: Fasudil exhibited a very potent vasodilatory effect on the RA compared with conventional papaverine or VG, resulting in increased GFF. This agent is useful for dilating RA grafts in CABG. © 2013 The Society of Thoracic Surgeons

Fasudil is a superior vasodilator for the internal thoracic artery in coronary surgery

Background: The internal thoracic artery (ITA) is a very useful conduit for coronary artery bypass artery (CABG), with excellent long-term patency. With the purpose to dilate the ITA graft and increase graft free flow (GFF) intraoperatively, we evaluated the usefulness of intraluminal injection of fasudil, a Rho-kinase inhibitor, in comparison to the conventional graft dilating agent, papaverine. Methods: Between June 2011 and January 2012, 30 patients with ischemic heart disease who underwent isolated CABG using ITA were enrolled. The patients were randomly assigned to 2 groups: the fasudil group (n = 15) in which fasudil solution 0.9 mg/dL was injected into the ITA, and the papaverine group (n = 15) in which papaverine solution (0.4 mg/mL) mixed with heparinized blood was used. Outcome measures were left ITA GFF, heart rate, and mean blood pressure during flow measurements, and histopathologic examination of the ITA. Results: In the fasudil group, GFF increased significantly (p < 0.01) from 19.7 ± 15.2 mL/minute at baseline to 66.9 ± 31.7 mL/minute after fasudil injection. In the papaverine group, GFF increased significantly (p < 0.01) from 22.9 ± 17.3 mL/minute at baseline to 44.8 ± 26.7 mL/minute after papaverine injection. Blood pressure and heart rate did not change significantly after drug injection in both groups. The GFF was significantly higher (p = 0.038) in fasudil-treated ITA than in papaverine-treated ITA. Histopathologically, the diameter of the ITA was markedly increased after fasudil injection. Elastica van Gieson staining showed that the multiple elastic lamellae structure was intact. Conclusions: Fasudil exhibited very potent vasodilatory effect on the ITA compared with conventional papaverine resulting in increased GFF. This agent is a useful graft dilating agent. © 2013 The Society of Thoracic Surgeons

Mild Heat Stress Affects on the Cell Wall Structure in Candida albicans Biofilm

福岡歯科大学2018年

A new instrument of suction support adapter system for epicardial radiofrequency ablation

ObjectiveRecently, epicardial ablation on a beating heart has been conducted as a minimally invasive surgical procedure for atrial fibrillation. We have developed a suction support adapter with the aim of ensuring contact between the ablation probe and the atrial wall to obtain transmural coagulation, and we evaluated the effectiveness of the adapter in electrophysiologic and histopathologic procedures.MethodsTo ensure contact between the radiofrequency ablation probe and the atrial wall, we designed and fabricated a suction support adapter. Twelve pigs were used in this study. A comparison was made between a group in which the ablation probe alone was used (group C, n = 6) and a group in which the ablation probe was used with the adapter (group S, n = 6). In both groups, epicardial ablation was conducted on a beating heart. An electrophysiologic evaluation was conducted to determine whether there was electrical isolation. Histopathologic evaluations were performed to identify the range of coagulation in the resected atrial wall specimens.ResultsThe coagulation range was significantly deeper in group S than in group C, and the breadth was significantly narrower. Transmural coagulation and electrical isolation of the atrial wall were seen in 1 case in group C and in all cases in group S.ConclusionsUsing the suction support adapter, we were able to maintain good contact between the ablation probe and the atrial wall. This device is thus thought to be useful for the safe and reliable performance of epicardial ablation on a beating heart