18 research outputs found
Exome, transcriptome and miRNA analysis donβt reveal any molecular markers of TKI efficacy in primary CML patients
Π ΠΎΠ»Ρ Π³Π°Π»Π΅ΠΊΡΠΈΠ½Π°-1 Π² ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ Π³ΠΎΠΌΠ΅ΠΎΡΡΠ°Π·Π° Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ²
Fault in T-lymphocytes homeostasis leads to different diseases with poor or vise versa strong immune response. So it seems to be interesting to research molecules of T-cell cooperation to develop new more effective therapeutic methods. Important factor modulating T-cell activity is galectin-1 which takes part in multiply process of cell biology β regulation of cell maturation, migration, signal transduction, functional ability and apoptosis.ΠΠ°ΡΡΡΠ΅Π½ΠΈΠ΅ Π³ΠΎΠΌΠ΅ΠΎΡΡΠ°Π·Π° Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΌΠΎΠΆΠ΅Ρ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡΡ ΠΊ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ, ΡΠ²ΡΠ·Π°Π½Π½ΡΡ
Ρ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΠΈΠ»ΠΈ, Π½Π°ΠΏΡΠΎΡΠΈΠ², Π³ΠΈΠΏΠ΅ΡΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ. Π ΡΠ²ΡΠ·ΠΈ Ρ ΡΡΠΈΠΌ ΠΎΡΠΎΠ±ΡΠΉ ΠΈΠ½ΡΠ΅ΡΠ΅Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ», ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ
Π² Π’-ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΊΠΎΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ, Ρ ΡΠ΅Π»ΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ². ΠΠ°ΠΆΠ½ΡΠΌ ΡΠ°ΠΊΡΠΎΡΠΎΠΌ ΠΌΠΎΠ΄ΡΠ»ΡΡΠΈΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΡΠ²Π»ΡΠ΅ΡΡΡ Π³Π°Π»Π΅ΠΊΡΠΈΠ½-1, ΠΊΠΎΡΠΎΡΡΠΉ Π²ΠΎΠ²Π»Π΅ΡΠ΅Π½ Π² ΠΌΠ½ΠΎΠ³ΠΎΡΠΈΡΠ»Π΅Π½Π½ΡΠ΅ ΠΏΡΠΎΡΠ΅ΡΡΡ ΠΆΠΈΠ·Π½Π΅Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ β ΡΠ΅Π³ΡΠ»ΡΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π°, ΠΌΠΈΠ³ΡΠ°ΡΠΈΡ, ΠΏΠ΅ΡΠ΅Π΄Π°ΡΡ ΠΌΠ΅ΠΆΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
ΡΠΈΠ³Π½Π°Π»ΠΎΠ², Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΎΡΠ½ΡΡ
ΡΡΠ½ΠΊΡΠΈΠΉ ΠΈ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΡ Π°ΠΏΠΎΠΏΡΠΎΠ·Π°
Π‘ΠΠ‘Π’ΠΠΠ ΠΠΠ£Π’ΠΠ’ΠΠΠΠ Π£Π§ΠΠ‘Π’ΠΠ£ΠΠ’ Π Π ΠΠΠ£ΠΠ―Π¦ΠΠ ΠΠΠΠΠ’ΠΠΠ ΠΠΠ£Π₯ΠΠΠΠΠ«Π₯ ΠΠΠΠ’ΠΠ
The research objective is to determine the role of the gluthatione system components in realization of the receptor pathway of Jukart tumor cell apoptosis.Apoptosis realization using FITC-labeled annexin V and propidium iodide as well as the amount of TNFΒ R1- and Fas-presenting cells has been evaluated by flow cytofluorometry; activity caspase-3 registered a spektroflyuorimetrichesky method. The concentration of reduced and oxidated gluthatione has been determined by spectrophotometry.The material for the research was intact Jukart tumor cells and the ones incubated in the presence of a selective inhibitor of the key gluthatione synthesis enzyme β buthionine-sulfoximine.The research has shown that the gluthatione system plays an important regulatory role in activation of the receptor pathway of Jukart tumor cell apoptosis.The gluthatione system components are targets for activation of programmed cell death in tumor growth.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β Π²ΡΡΠ²ΠΈΡΡ ΡΠΎΠ»Ρ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΡΠΈΡΡΠ΅ΠΌΡ Π³Π»ΡΡΠ°ΡΠΈΠΎΠ½Π° Π² ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΏΡΡΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Π»ΠΈΠ½ΠΈΠΈ Jurkat.ΠΡΠ΅Π½ΠΊΡ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΡΠ»ΡΠΎΡΠΈΠΌΠ΅ΡΡΠΈΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ FITS-ΠΌΠ΅ΡΠ΅Π½Π½ΠΎΠ³ΠΎ Π°Π½Π½Π΅ΠΊΡΠΈΠ½Π° V ΠΈ ΠΏΡΠΎΠΏΠΈΠ΄ΠΈΡ ΠΈΠΎΠ΄ΠΈΠ΄Π°, ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° TNFΒ R1- ΠΈ Fas-ΠΏΡΠ΅Π·Π΅Π½ΡΠΈΡΡΡΡΠΈΡ
ΠΊΠ»Π΅ΡΠΎΠΊ; Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΊΠ°ΡΠΏΠ°Π·Ρ-3 ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠ»ΡΠΎΡΠΈΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈ ΠΎΠΊΠΈΡΠ»Π΅Π½Π½ΠΎΠ³ΠΎ Π³Π»ΡΡΠ°ΡΠΈΠΎΠ½Π° ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠΌ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ»ΡΠΆΠΈΠ»ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ Π»ΠΈΠ½ΠΈΠΈ Jurkat: ΠΈΠ½ΡΠ°ΠΊΡΠ½ΡΠ΅ ΠΈ ΠΈΠ½ΠΊΡΠ±ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΠ° ΠΊΠ»ΡΡΠ΅Π²ΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΡΠΈΠ½ΡΠ΅Π·Π° Π³Π»ΡΡΠ°ΡΠΈΠΎΠ½Π° β Π±ΡΡΠΈΠΎΠ½ΠΈΠ½-ΡΡΠ»ΡΡΠΎΠΊΡΠΈΠΌΠΈΠ½Π°.ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΎ, ΡΡΠΎ ΡΠΈΡΡΠ΅ΠΌΠ° Π³Π»ΡΡΠ°ΡΠΈΠΎΠ½Π° ΠΈΠ³ΡΠ°Π΅Ρ Π²Π°ΠΆΠ½ΡΡ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ ΡΠΎΠ»Ρ Π² Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΏΡΡΠΈ Π°ΠΏΠΎΠΏΡΠΎΠ·Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Π»ΠΈΠ½ΠΈΠΈ Jurkat.ΠΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΡ ΡΠΈΡΡΠ΅ΠΌΡ Π³Π»ΡΡΠ°ΡΠΈΠΎΠ½Π° ΡΠ²Π»ΡΡΡΡΡ ΠΌΠΈΡΠ΅Π½ΡΠΌΠΈ Π΄Π»Ρ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π³ΠΈΠ±Π΅Π»ΠΈ ΠΏΡΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΌ ΡΠΎΡΡΠ΅
COGNITIVE IMPAIRMENT IN MULTIPLE SCLEROSIS: ASSOCIATION WITH THE ACTIVITY OF INFLAMMATORY PROCESS AND SEVERITY OF DISABILITY
Background: Multiple sclerosis is the most common neurological disease leading to disability in young and productive patients. One of available methods of control over the course of the disease and detection of its progression is neuropsychological testing. However, selection of the most informative tests, as well as the search for interpretation of their results is still ongoing.Aim: To study cognitive functions in multiple sclerosis patients depending on the disease activity and degree of disability.Materials and methods: Fifty patients with multiple sclerosis were evaluated during their remissions and 15 of them were additionally assessed during an exacerbation. During neuropsychological testing a computer test βSelection of figuresβ was used for assessment of attention, productivity, frontal functions and visual neglect. We studied potential dependency of the parameters obtained from degree of disability and disease stage (exacerbation or remission). The control group comprised 12 healthy subjects.Results: Compared to healthy subjects, the multiple sclerosis patients had a prolonged time of test performance, decreased learning, and statistically significant decrease of the working efficacy (p < 0.05). During an attack, there was a decreased conceptualization (48 answers compared to 51.5 at remission stage); more than 3-fold (from 1,3 to 4,4) increase in the number of figures missed in one visual field, that could indirectly indicate visual neglect. Also, changes in frontal functions were noted as a tendency to almost 2-fold higher numbers of perseverative answers, more frequent categorical escape and learning abnormality. There was a positive correlation between expanded disability scale score (EDSS) and working efficacy (r = 0.453, p = 0.001), and a negative correlation between EDSS and mental stability (r = 0.4055, p = 0.0035).Conclusion: Patients with multiple sclerosis had abnormal rate and accuracy of test performance, compared to those in healthy subjects. During an exacerbation of the disease, there was a deterioration of the parameters compared to those registered in the remission. Also, a negative association with disease severity assessed by EDSS, was found. The data obtained documents feasibility of the computer test βSelection of figuresβ for dynamic control in multiple sclerosis patients
ASSOCIATIONS OF INTERFERON GENE POLYMORPHISMS OAS-1, OAS-3, PKR WITH CHRONIC VIRAL HEPATITIS C
Abstract. Present work is devoted to analyzing possible associations of single-nucleotide gene polymorphisms (SNPs) with liver pathology in patients with chronic hepatitis C. SNP genotypes and allele frequencies were identified for some genes of interferon system, i.e., oligoadenilatsynthetase-1 (-1A/G), oligoadenilatsynthetase-3 (+1314C/T), and protein kinase R (+244A/G), using DNA samples from healthy donors and patients with chronic hepatitis C representing population of Tomsk and Tomsk Region. An association has been shown between SNPs of oligoadenilatsynthetase-1 and protein kinase R genes, and clinical activity of inflammatory process in the liver, as well as an impact of certain SNPs of protein kinase R and oligoadenilatsynthetase-3 genes upon fibrogenesis. (Med. Immunol., 2011, vol. 13, N 1, pp 93-100
ASSESSMENT OF COLOR VISION FOR DIAGNOSIS AND DYNAMIC MONITORING OF MULTIPLE SCLEROSIS
Background: Multiple sclerosis is regarded as the most frequent cause of neurological disability. Visual disturbances are common and may be due to pathology of retina, optic nerve and cerebral conduction tracts (optic tract). Routine methods of assessment of visual function are insufficient in diagnosing some visual problems including color vision disturbances. Aim: To assess color vision in patients with multiple sclerosis.Β Materials and methods: We examined 110 patients (age > 18 years old) with previously diagnosed multiple sclerosis. Neurological status was assessed using functional scales; results of neuroimaging, medical records and history were taken into account. Color vision was examined using Farnsworth dichotomous test. Control group included 20 healthy volunteers (8 men, 12 women, mean age 29.1 Β± 1.4 years old).Β Results: In patients with multiple sclerosis, color vision defects were significantly more prevalent compared to the control group (89.1% vs. 65%, p < 0.05). Deuteranopia was found in 18.6% of patients with multiple sclerosis, protanopia in 17.3%, tritanopia in 7.3% of patients. Significant color vision defects positively correlated with disability level of the Expanded Disability Status Scale (EDSS) and were independent of disease duration and history of retrobulbar neuritis.Β Conclusion: In patients with multiple sclerosis, color vision defects were associated with the activity of pathological process. Severity of neurological disability level estimated by EDSS positively correlated with visual disturbances. Thus, Farnsworth dichotomous test may be recommended for dynamic monitoring of multiple sclerosis
Molecular genetics of follicular cell thyroid carcinoma
Thyroid cancer is the most frequent endocrine malignancy. In the most cases thyroid cancer arises from follicular cells. Diagnosis of the cancer is based on the cytological analysis of fine needle aspiration biopsy of thyroid nodes. But the accuracy of the cytological diagnosis is about 80% that leads to the false positive and false negative cases and wrong strategy of treatment. Identification of genetic and epigenetic markers in the biopsies will allowΒ to improveΒ diagnostic accuracy. This article describes mutations, aberrant DNAΒ methylationΒ and abnormalΒ microRNAΒ expression constituting the core of molecular genetics of follicular cell thyroid cancer. The mutations given in the article includes point mutations, fusions and copy number variation. Besides frequent and well described driver mutations in genes ofΒ ΠΠΠ K, PI3K/Akt andΒ WntΒ signaling pathways, as well asΒ TP53Β andΒ TERTΒ genes, we introduce here less frequent mutations appeared in the literature during theΒ pastΒ two years. In addition the article contains examples ofΒ diagnostic panels applying these markers
The role of galectin-1 in the T-lymphocytes homeostasis
Fault in T-lymphocytes homeostasis leads to different diseases with poor or vise versa strong immune response. So it seems to be interesting to research molecules of T-cell cooperation to develop new more effective therapeutic methods. Important factor modulating T-cell activity is galectin-1 which takes part in multiply process of cell biology β regulation of cell maturation, migration, signal transduction, functional ability and apoptosis