Exploring Unconfirmed Transactions for Effective Bitcoin Address Clustering

peer reviewe

LiFePO4 Particles Embedded in Fast Bifunctional Conductor rGO&C@Li3V2(PO4)3 Nanosheets as Cathodes for High‐Performance Li‐Ion Hybrid Capacitors

The sluggish kinetics of Faradaic reactions in bulk electrodes is a significant obstacle to achieve high energy and power density in energy storage devices. Herein, a composite of LiFePO 4 particles trapped in fast bifunctional conductor rGO&C@Li 3 V 2 (PO 4 ) 3 nanosheets is prepared through an in situ competitive redox reaction. The composite exhibits extraordinary rate capability (71 mAh g −1 at 15 A g −1 ) and remarkable cycling stability (0.03% decay per cycle over 1000 cycles at 10 A g −1 ). Improved extrinsic pseudocapacitive contribution is the origin of fast kinetics, which endows this composite with high energy and power density, since the unique 2D nanosheets and embedded ultrafine LiFePO 4 nanoparticles can shorten the ion and electron diffusion length. Even applied to Li-ion hybrid capacitors, the obtained devices still achieve high power density of 3.36 kW kg −1 along with high energy density up to 77.8 Wh kg −1 . Density functional theory computations also validate that the remarkable rate performance is facilitated by the desirable ionic and electronic conductivity of the composite

Data_Sheet_1_Associations of COVID-19 vaccination during pregnancy with adverse neonatal and maternal outcomes: A systematic review and meta-analysis.PDF

ObjectivesThe low COVID-19 vaccine uptake rate among pregnant women is mainly due to safety concerns about COVID-19 vaccines due to limited safety evidence. Our goal was to evaluate the safety of COVID-19 vaccination during pregnancy with up-to-date evidence.MethodsA comprehensive search of MEDLINE, EMBASE, the Cochrane Library, and clinicaltrials.gov was performed on April 5th, 2022, and updated on May 25th, 2022. Studies evaluating the association of COVID-19 vaccination during pregnancy with adverse maternal and neonatal outcomes were included. Two reviewers independently performed the risk of bias assessment and data extraction. Inverse variance random effect meta-analyses were performed to pool outcome data.ResultsForty-three observational studies were included. COVID-19 vaccination [96,384 (73.9%) BNT162b2, 30,889 (23.7%) mRNA-1273, and 3,172 (2.4%) other types] during pregnancy [23,721 (18.3%) in the first trimester, 52,778 (40.5%) in the second trimester, and 53,886 (41.2%) in the third trimester].was associated with reduced risks of stillbirth or neonatal death (OR, 0.74; 95% CI, 0.60–0.92). Sensitivity analysis restricted to studies in participants without COVID-19 showed that the pooled effect was not robust. COVID-19 vaccination during pregnancy was not associated with congenital anomalies (OR, 0.83; 95% CI, 0.63–1.08), preterm birth (OR, 0.98; 95% CI, 0.90–1.06), NICU admission or hospitalization (OR, 0.94; 95% CI, 0.84–1.04), an Apgar score at 5 min ConclusionsCOVID-19 vaccination during pregnancy was not associated with any of the adverse neonatal or maternal outcomes studied. Interpretation of study findings is limited by the types and timing of vaccination. The vaccinations in our study received during pregnancy were primarily mRNA vaccines administered in the second and third trimester. Future RCTs and meta-analysis are warranted to evaluate the efficacy and long-term effects of the COVID-19 vaccines.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022322525, identifier: PROSPERO, CRD42022322525.</p

An oleaginous yeast platform for renewable 1-butanol synthesis based on a heterologous CoA-dependent pathway and an endogenous pathway

Abstract Background Microbial biofuel production provides a promising sustainable alternative to fossil fuels. 1-Butanol is recognized as an advanced biofuel and is gaining attention as an ideal green replacement for gasoline. In this proof-of-principle study, the oleaginous yeast Yarrowia lipolytica was first engineered with a heterologous CoA-dependent pathway and an endogenous pathway, respectively. Results The co-overexpression of two heterologous genes ETR1 and EutE resulted in the production of 1-butanol at a concentration of 65 μg/L. Through the overexpression of multiple 1-butanol pathway genes, the titer was increased to 92 μg/L. Cofactor engineering through endogenous overexpression of a glyceraldehyde-3-phosphate dehydrogenase and a malate dehydrogenase further led to titer improvements to 121 μg/L and 110 μg/L, respectively. In addition, the presence of an endogenous 1-butanol production pathway and a gene involved in the regulation of 1-butanol production was successfully identified in Y. lipolytica. The highest titer of 123.0 mg/L was obtained through this endogenous route by combining a pathway gene overexpression strategy. Conclusions This study represents the first report on 1-butanol biosynthesis in Y. lipolytica. The results obtained in this work lay the foundation for future engineering of the pathways to optimize 1-butanol production in Y. lipolytica

Suppression of cytochrome p450 reductase enhances long-term hematopoietic stem cell repopulation efficiency in mice.

BACKGROUND: Bone marrow microenvironment (niche) plays essential roles in the fate of hematopoietic stem cells (HSCs). Intracellular and extracellular redox metabolic microenvironment is one of the critical factors for the maintenance of the niche. Cytochrome P450 reductase (CPR) is an obligate electron donor to all microsomal cytochrome P450 enzymes (P450 or CYP), and contributes to the redox metabolic process. However, its role in maintaining HSCs is unknown. OBJECTIVE: To examine the effects of low CPR expression on HSCs function using a mouse model of globally suppressed Cpr gene expression (Cpr Low, CL mice). METHODS: Hematopoietic cell subpopulations in bone marrow (BM) and peripheral blood (PB) from WT and CL mice were examined for their repopulation and differentiation ability upon BM competitive transplantation and enriched HSC (LKS(+)) transplantation. Effects of low CPR expression on hematopoiesis were examined by transplanting normal BM cells into CL recipients. Reactive oxygen species (ROS), cell cycle, and apoptosis in CL mice were analyzed by flow cytometry for DCF-DA fluorescence intensity, Ki67 protein, and Annexin-V, respectively. RESULTS: The levels of ROS in BM cells, HPCs and HSCs were comparable between CL and WT mice. In comparison to WT mice, the number of LT-HSCs or ST-HSCs was lower in CL mice while CMPs, GMPs and MEPs in CL mice were higher than that in WT control. Competitive transplantation assay revealed enhanced repopulation capacity of HSCs with low CPR expression, but no difference in differentiation potential upon in vitro experiments. Furthermore, lymphoid differentiation of donor cells decreased while their myeloid differentiation increased under CL microenvironment although the overall level of donor hematopoietic repopulation was not significantly altered. CONCLUSIONS: Our studies demonstrate that suppressing CPR expression enhances the repopulation efficiency of HSCs and a low CPR expression microenvironment favors the differentiation of myeloid over lymphoid lineage cells

Leukemic marrow infiltration reveals a novel role for Egr3 as a potent inhibitor of normal hematopoietic stem cell proliferation

Cytopenias resulting from the impaired generation of normal blood cells from hematopoietic precursors are important contributors to morbidity and mortality in patients with leukemia. However, the process by which normal hematopoietic cells are overtaken by emerging leukemia cells and how different subsets of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) are distinctly influenced during leukemic cell infiltration is poorly understood. To investigate these important questions, we used a robust nonirradiated mouse model of human MLL-AF9 leukemia to examine the suppression of HSCs and HPCs during leukemia cell expansion in vivo. Among all the hematopoietic subsets, long-term repopulating HSCs were the least reduced, whereas megakaryocytic-erythroid progenitors were the most significantly suppressed. Notably, nearly all of the HSCs were forced into a noncycling state in leukemic marrow at late stages, but their reconstitution potential appeared to be intact upon transplantation into nonleukemic hosts. Gene expression profiling and further functional validation revealed that Egr3 was a strong limiting factor for the proliferative potential of HSCs. Therefore, this study provides not only a molecular basis for the more tightened quiescence of HSCs in leukemia, but also a novel approach for defining functional regulators of HSCs in disease

Romanian journal of psychoanalysis : = Revue Roumain de Psychanalyse

Course-dependent chemotherapy response of the T-ALL mice. When leukemic cells reached 1-5% in PB, mice received chemotherapy composed of CTX (100 mg/kg) and Ara-C (150 mg/kg) for a consecutive 1, 2, 3 and 4 days, respectively. (A) Median survival days post leukemic cell injection were 29, 39.5, 45 and 71.5 for the leukemia-only, one-day treated, two-day treated and three-day treated group, respectively (n = 6-10). For the four-day treated group, no mice died within the inspecting 90 days (n = 6). (B) Leukemic burden in PB of the four differently treating groups (n = 6). Data showed that longer the therapeutic course, longer the relapse-free period. The day on which leukemic cells showed up again in PB for the one-day, two-day and three-day treated groups were the 8th, 11th and 17th day post therapy, respectively. While for the four-day treated group, no appearance of relapse was detected within the inspecting 90 days. (C) White blood cell count in PB of the four differently treating groups (n = 6). Data showed that longer the therapeutic course, heavier the depression of white blood cells post therapy. (D) Platelet count in PB of the four differently treating groups (n = 6). Data showed that longer the therapeutic course, longer the suppression period of platelet in PB post therapy. All data were presented as meanÂąSEM. Statistical significance as: * p<0.05; ** p<0.01; *** p<0.001

Similar ROS levels in BM cells between CL and WT mice.

<p>(<b>A</b>) CPR protein levels in bone marrow mononuclear cells (BMMNCs) and LKS⁺ of CL and WT mice were analyzed by Western blotting. (<b>B</b>) CPR mRNA expression in whole bone marrow cells (WBMCs), LKS⁺ and CD34⁻LKS⁺ were detected by qRT-PCR. Data shown are mean ± SEM (*P<0.05, **P<0.01, ***P<0.001, n3). (<b>C–D</b>) Representative FACS profiles (C) and quantification of the levels of ROS (D) in BM, LKS⁻ and LKS⁺ cells determined by DCF-DA mean fluorescence intensity with flow cytometry. Data shown are representative of two independent experiments.</p

Improved reconstitution of CL WBMCs during serial transplantation.

<p>1<b>^st</b> transplantation: 5×10⁵ WBMCs from WT or CL mice plus 5×10⁵ CD45.1 WBMCs were transplanted into lethally irradiated (9.5 Gy) recipients (B6.SJL, CD45.1). (<b>A–B</b>) The percentage of CD45.2 donor cell in PB and BM at 4-week interval after transplantation. Data shown are mean ± SEM (n = 5–8). 2^nd transplantation: sorted 5×10⁵ CD45.2 cells from 1^st recipients of WT or CL mice, plus 5×10⁵ CD45.1 WBMCs were transplanted into lethally irradiated (9.5 Gy) recipients (B6.SJL, CD45.1). (<b>C–D</b>) The percentage of CD45.2 donor cell in PB and BM was analyzed every 4 weeks after transplantation. Data shown are mean ± SEM (**P<0.01, n = 3–4).</p