3 research outputs found
Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
The aim of this study was to evaluate the susceptibility of 35
resistant Pseudomonas aeruginosa clinical isolates to a
quaternary ammonium hospital disinfectant. The methodology was the AOAC
Use-Dilution Test, with disinfectant at its use-concentration. In
addition, the chromosomal DNA profile of the isolates were determined
by macro-restriction pulsed field gel electrophoresis (PFGE) method
aiming to verify the relatedness among them and the behavior of
isolates from the same group regarding the susceptibility to the
disinfectant. Seventy one percent of the isolates were multiresistant
to antibiotics and 43% showed a reduced susceptibility to the
disinfectant. The PFGE methodology detected 18 major clonal groups. We
found isolates with reduced susceptibility to the disinfectant and we
think that these are worrying data that should be further investigated
including different organisms and chemical agents in order to
demonstrate that microorganisms can be destroyed by biocide as
necessary. We also found strains of the same clonal groups showing
different susceptibility to the disinfectant. This is an interesting
observation considering that only few works are available about this
subject. PFGE profile seems not to be a reliable marker for resistance
to disinfectants