Designing two synthetic constructs for real time pcr detection of francisella tularensis and ebola virus

Background: Generally, timely diagnosis of micro-organisms is very important to prevent many diseases. Many methods can detect micro-organisms like culture-based methods and molecular methods. The molecular methods are usually preferred be-cause they provide fast and reliable results. In some cases, microbial strains are not ac-cessible, and there is no safety to work with them; therefore, synthetic constructs which are designed according to the available sequences in databases can be used as a positive control for detection of them. Methods: In this study, a synthetic construct was designed for molecular detection of Francisella tularensis (F. tularensis) and the Ebola virus by multiplex real-time PCR reaction. For this, sequences were taken from databases and then multiple alignments were done by software. Also, conventional PCR and two models of real-time PCR (SYBR green and TaqMan) were applied. Finally, multiplex real-time PCR was performed. Results: The synthetic construct was designed and used for conventional PCR and multiplex PCR. The results of common PCR showed a single band at 148 bp and 167 bp in 1.5 agarose gel stained by ethidium bromide for F. tularensis and Ebola virus, respectively. Also, a dual-band at 148 and 167 bp was observed in multiplex PCR. Results of real-time PCR showed a limit of detection about 0.1 pg of plasmid/µl. Conclusion: In conclusion, the designed construct can be used as a positive control for an accurate diagnosis of these micro-organisms without any biological danger for laboratory staff. So, this method is useful for diagnosis of these agents in food, water, and blood samples. © 2021, Avicenna Journal of Medical Biotechnology. All rights reserved

Stator Turn-to-Turn Fault Detection of Induction Motor by Non-Invasive Method Using Generalized Regression Neural Network

Condition monitoring and protection methods based on the analysis of the machine's current are widely used according to non-invasive characteristics of current transformers. It should be noted that, these sensors are installed by default in the machine control center. On the other hand, condition monitoring based on mathematical methods has been proposed in literature. However, they are model based and are too complex. Artificial neural network (ANN) methods are robust and less model dependent for fault diagnosis when the fault signature can be directly achieved using the sampling data. In this procedure, the state of internal process will be ignored. Therefore, generalized regression neural network (GRNN) based method is presented in this paper that uses negative sequence currents (calculated from the machine's currents) as inputs to detect and locate an inter-turn fault in the stator windings of the induction motor. Turn-to-turn fault by changing the contact resistance and various numbers of shorted turns for realizing the fault severity has been modeled by Matlab/Simulink. The simulation and experimental results show that the proposed method is effective for the diagnosis of stator inter-turn fault in induction motor under the supply voltage unbalances

Study on Application of Two Different Magnetic Materials in Rotor of Cylindrical Synchronous Generator to Produce Reluctance Torque

Synchronous generators are of two type’s salient pole type and round rotor type. The load angle curve of a cylindrical rotor synchronous machine comprises a single sine term only while in salient pole synchronous generators, power-angle characteristic has two terms. The first term is the fundamental component due to field excitation (the same as the cylindrical rotor) and the second term includes the effect of salient pole. In fact, this term is the second harmonic component due to reluctance torque. This paper presents a study on the new design of cylindrical solid rotor synchronous generator. In this new design, rotor of the machine is designed in such a way that the required inductance values are reached to produce reluctance torque, besides electromagnetic torque due to field excitation. In this contribution, a combination of two different ferromagnetic materials is considered in the design of the rotor. In this theory, the tight connection between the different materials is very important from a mechanical point of view. In other words, this new idea and production principal has potential in some areas after some further research and engineering. But this paper is focused on magnetic flux-carrying materials and presents a study of the new design of cylindrical solid rotor synchronous generator (NCG). Then a comparative analysis was made between this new (NCG) and conventional cylindrical solid rotor synchronous generator (CCG) and the effectiveness of the new cylindrical solid rotor from a magnetic point of view is demonstrated. In this paper, mechanical and thermal aspects of design such as vibration did not analyze

Loss of Excitation Detection in Doubly Fed Induction Generator by Voltage and Reactive Power Rate

The doubly fed induction generator (DFIG) is one of the most popular technologies used in wind power systems. With the growing use of DFIGs and increasing power system dependence on them in recent years, protecting of these generators against internal faults is more considered. Loss of excitation (LOE) event is among the most frequent failures in electric generators. However, LOE detection studies heretofore were usually confined to synchronous generators. Common LOE detection methods are based on impedance trajectory which makes the system slow and also prone to interpret a stable power swing (SPS) as a LOE fault. This paper suggests a new method to detect the LOE based on the measured variables from the DFIG terminal. In this combined method for LOE detection, the rate of change of both the terminal voltage and the output reactive power are utilized and for SPS detection, the fast Fourier transform (FFT) analysis of the output instantaneous active power has been used. The performance of the proposed method was evaluated using Matlab/Simulink interface for various power capacities and operating conditions. The results proved the method's quickness, simplicity and security

Geometry optimization of five-phase permanent magnet synchronous motors using Bees algorithm

Among all types of electrical motors, permanent magnet synchronous motors (PMSMs) are reliable and efficient motors in industrial applications. Because of their superiority over other kinds of motors, they are replacing conventional electric motors. On the other hand, high-phase PMSMs are good candidates to be used in certain industrial and military projects such as electric vehicles, spacecrafts, naval systems and etc. In these cases, the motor has to be designed with minimum volume and high torque and efficiency. Design optimization can improve their features noticeably, thus reduce volume and enhance performance of motors. In this paper, a new method for optimum design of a five-phase surface-mounted permanent magnet synchronous motor is presented to achieve minimum permanent magnets (PMs) volume with an increased torque and efficiency. Design optimization is performed in search for optimum dimensions of the motor and its permanent magnets using Bees Algorithm (BA). The design optimization results in a motor with great improvement regarding the original motor which is compared with two well-known evolutionary algorithms i.e. GA and PSO. Finally, finite element method simulation is utilized to validate the accuracy of the design

Analysis of Magnetic Flux Linkage Distribution in Salient-Pole Synchronous Generator with Different Kinds of Inter-Turn Winding Faults

A reliable and accurate diagnosis of inter-turn short circuit faults is a challenging problem in the area of fault diagnosis of electrical machines. The purpose of this challenge is to be more efficient in fault detection and to provide a reliable method with low-cost sensors and simple numerical algorithms which not only detect the occurrence of the fault, but also locate its position in the winding. Hence, this paper presents a novel method for diagnosis of different kinds of inter-turn winding faults in a salient-pole synchronous generator using the change in the magnetic flux linkage. It describes the influence of inter-turn winding faults on the magnetic flux linkage distribution of the generator. The main feature of the proposed method is its capability to identify the faulty coils under two types of inter-turn winding faults. Also, simple algorithm, low cost sensor and sensitivity are the other feature in the proposed technique. In this method, generator air gap flux linkage is measured via search coils sensor installed under the stator wedges. Theoretical approach based on Finite Element Method (FEM) together with experimental results derived from a 4-pole, 380U, 1500 rpm, 50 Hz, 50 KVA, 3-phase salient-pole synchronous generator confirm the validity of the proposed method

Modelling and Simulation of a Transformer With Inter-turn Fault Including Saturation Effect and Variable Fault Parameters

This investigation deals with a mathematical model for a distribution transformer including saturation effect. To this end, the equations related to a three phase transformer are specified and the effect of an inter-turn fault is included. Naturally by applying an inter-turn fault the inductance and resistance matrix will change. Thus, unknown quantities of inductances and resistances for completing the matrix are calculated and the inputs, outputs and state variables are specified. All the equations will be rewritten in terms of state variables, subsequently saturation effect is added to the model. Finally the block diagram of the specified model based on the obtained equations are designed and the ultimate model is simulated. The saturation effect, added to the mathematical model and also the variable fault parameters are known as two significant contributions which distinguish this study from other investigations. Various results obtained from the simulation of the final model confirm the changes in the behavior of faulty transformer such as: a large circulating current flowing in the shorted turns, lower impact on terminal voltages and currents, a sudden increase in current flowing in the primary winding, asymmetrical flux distribution and inverse proportion of the fault severity and the limiting resistor