カワウ捕食圧下におけるアユ管理のための確率制御モデル

京都大学0048新制・課程博士博士(農学)甲第22488号農博第2392号新制||農||1076(附属図書館)学位論文||R2||N5268(農学部図書室)京都大学大学院農学研究科地域環境科学専攻(主査)教授 藤原 正幸, 教授 村上 章, 准教授 宇波 耕一学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDFA

A potential diagnostic biomarker: Proteasome LMP2/b1i-differential expression in human uterus neoplasm

Uterine leiomyosarcoma (ULMS) develops more often in the muscle tissue layer of the uterine body than in the uterine cervix. The development of gynecologic tumors is often correlated with female hormone secretion; however, the development of uterine ULMS is not substantially correlated with hormonal conditions, and the risk factors are not yet known. Importantly, a diagnostic-biomarker which distinguishes malignant ULMS from benign tumor leiomyoma (LMA) is yet to be established. Accordingly, it is necessary to analyze risk factors associated with uterine ULMS, to establish a treatment method. Proteasome low-molecular mass polypeptide 2(LMP2)/b1i-deficient mice spontaneously develop uterine LMS, with a disease prevalence of ~40% by 14 months of age. We found LMP2/b1i expression to be absent in human LMS, but present in human LMA. Therefore, defective-LMP2/b1i expression may be one of the risk factors for ULMS. LMP2/b1i is a potential diagnostic-biomarker for uterine ULMS, and may be a targeted-molecule for a new therapeutic approach

Transient expression of the coat protein of Apple chlorotic leaf spot virus inhibits the viral RNA accumulation in Nicotiana occidentalis

The coat protein of Apple chlorotic leaf spot virus (ACLSV-CP) plays a crucial role in infectivity and efficient viral RNA accumulation in host cells (J. Gen. Virol, 88, 2007). In this study, the effect of ACLSV-CP on viral RNA accumulation in Nicotiana occidentalis was investigated. The CP, CPm40 (an amino acid (aa) substitution of Ala to Ser at aa position 40), CPm75 (a substitution of Phe to Tyr at aa position 75), and CPm40m75 (two aa substitutions at positions 40 and 75) of ACLSV (P205) were transiently expressed in N. occidentalis leaves by agroinfiltration. Immunoblot analysis showed that CP and CPm40m75 accumulated in infiltrated tissues, in contrast to CPm40 and CPm75 which were not detected, suggesting that the stable accumulation of CP is important for effective viral RNA accumulation. However, co-agroinfiltration of an infectious ACLSV cDNA clone (pBICLSF) or pBICLSF-based CP mutants (pBICLCPm40, pBICLCPm75, and pBICLCPm40m75) with a vector expressing CP (pBE2113-CP) showed no viral genomic RNA accumulations were found in any leaves infiltrated with these constructs. The inhibition of ACLSV-RNA accumulation was found only in leaves co-expressed with CP protein, but not with a frame-shift mutant of CP, a movement protein (P50), and a frame-shift mutant of P50. Keywords: Apple chlorotic leaf spot virus, coat protein, protein stability, coat protein mediated resistance (CP-MR), agroinfiltratio

Enhancement of Near-Infrared Emission Based on Hypervalent Germanium(IV)-Fused Azobenzene Compounds with Electron-Donating Groups

NIR-light emitting materials are significant as a source for NIR-light technology. Previously, we have found that hypervalent germanium-fused azobenzene (GAz) compounds showed NIR emissions despite the small π-conjugated systems owing to trigonal bipyramidal geometry with three-center four-electron (3c-4e) bonds. Herein, we succeed in enhancing their luminescence properties by introduction of electron-donating groups. It is revealed that the frontier molecular orbitals of the π-conjugated systems with the hypervalent bonds can be varied depending on the type of substituents. As a result, a GAz compound with diphenylamine groups shows efficient NIR emission at room temperature (λᴘʟ = 761 nm and Φᴘʟ = 0.07) and at 77 K (λᴘʟ = 749 nm and Φᴘʟ = 0.20). Furthermore, data from theoretical calculations support the experimental results and provide additional information on the producibility of the hypervalent compounds. Our findings afford strategies to expand the library of the fine-tunable NIR-luminescent materials involving heteroatoms

Red blood cell distribution width is a predictive factor of anthracycline-induced cardiotoxicity

博士（医学）福島県立医科大