Role of vitamin C on hormonal and pathological changes in Heteropneustes fossilis (Bloch) due to exposure to sodium fluoride

706-713Fluoride is an essential trace element for humans and animals. Here, we explored the effects of sodium fluoride (NaF) on morphological, hormonal and histological changes in ovary of Asian stinging catfish Heteropneustes fossilis (Bloch) after exposure to 1/10th mg and 1/5th mg F/L of LC50 of fluoride in water for 45 and 90 days. Activities of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T) and estradiol (E) were measured using an enzyme-linked immunosorbent assay. Histological evaluation of the ovaries was conducted after staining with haematoxylin-eosin (HE). Reproductive hormones were significantly decreased after NaF-treated groups. Ovary was found to be adversely effected by exposure to NaF. Maturation of follicles was inhibited by exposure after observation of GSI and histology in ovary. These results suggest that female reproductive system is adversely affected by NaF. NaF may thus significantly reduce the fertility of female fish. This study encompasses alteration in hormonal assay to evaluate the role of vitamins C in the recovery of fluoride toxicity in fish. Vitamin C is a water-soluble ROS scavenger with high potency

Role of vitamin C on hormonal and pathological changes in Heteropneustes fossilis (Bloch) due to exposure to sodium fluoride

Fluoride is an essential trace element for humans and animals. Here, we explored the effects of sodium fluoride (NaF) on morphological, hormonal and histological changes in ovary of Asian stinging catfish Heteropneustes fossilis (Bloch) after exposure to 1/10th mg and 1/5th mg F/L of LC50 of fluoride in water for 45 and 90 days. Activities of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T) and estradiol (E) were measured using an enzyme-linked immunosorbent assay. Histological evaluation of the ovaries was conducted after staining with haematoxylin-eosin (HE). Reproductive hormones were significantly decreased after NaF-treated groups. Ovary was found to be adversely effected by exposure to NaF. Maturation of follicles was inhibited by exposure after observation of GSI and histology in ovary. These results suggest that female reproductive system is adversely affected by NaF. NaF may thus significantly reduce the fertility of female fish. This study encompasses alteration in hormonal assay to evaluate the role of vitamins C in the recovery of fluoride toxicity in fish. Vitamin C is a water-soluble ROS scavenger with high potency

Feto-maternal outcomes following caesarean section: a prospective comparative study at tertiary care centre in North-Western Rajasthan

Background: Caesarean section (CS) is employed when vaginal delivery is not feasible or hazardous to the mother and/or her baby. The procedure, however, is not without risk. We determined the fetomaternal outcomes of CS conducted at P.B.M Tertiary hospital situated in the North-Western region of Rajasthan.Methods: This is a Hospital based prospective comparative study of all CSs performed for various indications at the Dept. of Gynaecology and Obst., S.P. Medical College and P.B.M Hospital, Bikaner, India, from August 01, 2016, to July 31, 2017. All patients who had CS at any time within the 24 h period were noted and followed up until discharge. The sociodemographic data, types of CS, indications, and feto-maternal outcomes were documented in a proforma. Statistical analysis was carried out using the SPSS version 24.Results: There were 16386 deliveries out of which 4456 (27.1%) were by LSCS. The age range of the group A was 21-25 years while in group B it was 26-30 years. The mean age group A was 22.4, and group B it was 27.9 years. Total 6572 primigravida patients delivered and 32.1% had LSCS. Total 9814 multigravida patients delivered and 12.6% had primary LSCS. In group A, 119(79.3%) LSCS were elective as compared to group B where only 19(12.7%) were elective and this difference was found statistically highly significant (p<0.001). Indication of LSCS is different in both the groups. Fetal distress was most common indication in group A (53.3%) while in group B most common indication was APH (35.9%). Perinatal mortality/morbidity was significantly higher in group B (7.3%) as compared to group A (2.7%).Conclusions: The CS rate in this study was 27.1%. Although primary caesarean section in multipara constitutes only a small percentage of total deliveries and caesarean, they are associated with high maternal and perinatal morbidity. The reason for these complications is many. Beside obstetrical causes, factors like lack of antenatal care, low socioeconomic status, anaemia, malnutrition and illiteracy also play a major role obstructed labor and previous CS among Maternal and perinatal complications were more frequent with emergency CS and in the referred cases

Immunohistochemical expression of SNAIL and SLUG in endometrioid endometrial cancer and precursor lesions

Background: Endometrioid endometrial carcinoma is one of the most prevalent gynecologic cancers. The epithelial-mesenchymal transition is a multistep process involved in the development of cells that results in disappearance of intercellular adhesion in the epithelium and acquiring mesenchymal properties, hence influence cancer progression and metastasis. Loss of intercellular adhesion can be activated by different mechanisms, including transcriptional repression. However, the expression of transcriptional repressors in EEC and precursor lesions remain to be investigated. In the present study, we evaluated the immunohistochemical expression of proteins of transcriptional after repression snail and slug in EEC and its precursor lesions. Methods: It was a prospective nested case-control study on women from 35 to 70 years of age. Endometrial biopsies were obtained and processed for routine histological diagnosis. Immunohistochemical expression of snail and slug were evaluated. Results: A total of 39 EEC cases, 37 Endometrial hyperplasia and 19 normal controls were included in this study. Expression of snail was positive in 77% (30/39) and slug in 82% (32/39) of EEC cases while in precancer group snail was positive in 76% (28/37) and slug in 68% (25/37). In normal control snail was positive in 32% (6/19) and SLUG in 21% (4/19). Conclusion: Up-regulation of snail &amp; slug observed in both precancer and cancer cases, suggesting their involvement from an early stage of carcinogenesis. Therefore therapies targeted at transcriptional repressors at an early stage of carcinogenesis, i.e., at precancerous lesions, could play a valuable role in reducing cancer progression and metastasis

A study on delayed cord clamping and early skin-to-skin contact and its effects on neonatal outcome

Background: Previous study conducted against immediate tying and cutting of the umbilical cord and suggested waiting until the child had taken repeated breaths and the pulsation in the cord had ceased to prevent potential weakness in the child.  A comparative study between delayed cord clamping (DCC) and early cord clamping (ECC) was carried out on a select group of primigravida without any high-risk factor and delivering at term. The objective of the study was to identify the effects of delayed cord clamping and early skin to skin contact on new born infant’s physiological parameters (temperature, weight, SpO2= saturation of peripheral oxygen, Apgar score= appearance, pulse, grimace, activity, and respiration, Hb=haemoglobin level). Methods: Study sample consisted of 300 mothers and their new born after gaining mother’s acceptance. They were divided into 2 groups of 150 each. Group A underwent delayed cord clamping and early skin to skin contact and group B early cord clamping. Newborns monitored 24 hours for hypothermia, apnoea, oxygen needs. Results: The findings of the present study were equivalent among both groups regards the mean neonatal haematological parameters were comparable and slightly elevated hemoglobin level and weight status among late cord clamping compared to early cord clamping group with significant difference was observed at 24 hour later. Conclusions: This study was found that DCC does have a beneficial effect on temperature, Apgar score, SpO2, Hb level and weight status of new born. Therefore, it is believed that DCC and early skin to skin contact (ESSC) provides effective thermal control with a reduced risk of hypothermia

Musk Rose (Rosa moschata) Grown at Middle Hill Climatic Conditions of the Western Himalayas: A Rich Source of Anti-Oxidants and Nutrients

236-243Musk rose (Rosa moschata) is an important wild aromatic climbing shrub of Rosaceae family. Scant scientific information is available on phyto-chemical composition and antioxidant activity of the Himalayan plant. Here we report, nutrient compositions, antioxidant constituents and activities of the different plant parts like leaves, petals, and rest of the flower parts of musk rose. Chlorophyll pigments and carotenoids contents were significantly higher in the mature leaves (2113.1 and 125.2 mg/g fresh weight respectively). The mature leaves also contained significantly higher amount of ascorbic acid (227.0 mg/100g) followed by the dried petals (141.6 mg/100g). The total antioxidant activity, determined based on ABTS and DPPH free radical scavenging ability, was higher in the hydro-alcoholic extracts from dried petals, dried flowers and mature leaves than that in the other parts. Analysis of the extracts from the dried petals revealed significantly higher contents of phenolics (62.9 mg/g), flavonoids (23.7 mg/g), tannins (133.8 mg/g), total carbohydrates (191.6 mg/g) and soluble protein (49.3 mg/g). The mature leaves were found to be the next richer sources for these nutrients. Correlation analysis revealed positive correlation of total antioxidant activities and the antioxidant constituents of the plant extract suggesting contribution of these compounds to the higher antioxidant activities. Thus, the phyto-chemical composition analyses suggest dried petals and mature leaves as potential natural sources of the nutrients and antioxidants. The study may further enhance utility of the Himalayan plant as source for preparing alternative natural medicines and functional foods