Formation of Norisoprenoid Flavor Compounds in Carrot (Daucus carota L.) Roots: Characterization of a Cyclic-Specific Carotenoid Cleavage Dioxygenase 1 Gene

Carotenoids are isoprenoid pigments that upon oxidative cleavage lead to the production of norisoprenoids that have profound effect on flavor and aromas of agricultural products. The biosynthetic pathway to norisoprenoids in carrots (Daucus carota L.) is still largely unknown. We found the volatile norisoprenoids farnesylacetone, α-ionone, and β-ionone accumulated in Nairobi, Rothild, and Purple Haze cultivars but not in Yellowstone and Creme de Lite in a pattern reflecting their carotenoid content. A cDNA encoding a protein with carotenoid cleavage dioxygenase activity, <i>Dc</i>CCD1, was identified in carrot and was overexpressed in Escherichia coli strains previously engineered to produce different carotenoids. The recombinant <i>Dc</i>CCD1 enzyme cleaves cyclic carotenes to generate α- and β-ionone. No cleavage products were found when <i>Dc</i>CCD1 was co-expressed in E. coli strains accumulating non-cyclic carotenoids, such as phytoene or lycopene. Our results suggest a role for <i>Dc</i>CCD1 in carrot flavor biosynthesis

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28 avril 19391939/04/28 (A68).Appartient à l’ensemble documentaire : PoitouCh

Additional file 5: Table S4. of A bulk segregant transcriptome analysis reveals metabolic and cellular processes associated with Orange allelic variation and fruit β-carotene accumulation in melon fruit

List of 79, 805, 37 and 122 genes that were differentially expressed at 10, 20, 30 DAA, and the mature stage, respectively. (XLSX 162 kb

Additional file 6: Table S5. of A bulk segregant transcriptome analysis reveals metabolic and cellular processes associated with Orange allelic variation and fruit β-carotene accumulation in melon fruit

List of genes exhibited significant up or down regulation in two fruit developmental stages. (XLSX 10 kb