The number of samples positive for each pair of pathogens.

<p>The number of samples positive for each pair of pathogens.</p

Sequences of primers and probes used in the study.

<p>Sequences of primers and probes used in the study.</p

Correlation between real-time PCR and the RBPT results.

<p>The mean Ct-value of samples negative for <i>Brucella</i> (5.95) was significantly lower than that of samples with a strong reaction results (24.71), which is lower than that of samples with a weak reaction (34.61) (p<0,0001).</p

Sequencing results of vaginal swab samples positive with the pan-<i>Chlamydiales</i> PCR.

<p>*These results were previously published by Barkallah et al. (2013).</p

The association of abortion with positive results for different agents and frequencies of positive results between vaginal and milk samples.

<p>The association of abortion with positive results for different agents by real-time PCR and frequencies of positive results between vaginal and milk samples were assessed using the Fisher's exact test. A p value<0.05 was taken as a level of significance. Cases: cows with clinical signs, controls: cows with normal pregnancies and normal parturitions, OR: odds ratio, CI: confidence interval.</p

Details of cases positive for <i>Brucella abortus</i>.

<p>+ positive/ − negative.</p