Additional file 1: of SPIN1, negatively regulated by miR-148/152, enhances Adriamycin resistance via upregulating drug metabolizing enzymes and transporter in breast cancer

Figure S1 and Figure S2. SPIN1 expression in breast cancer cells and miR-148a-3p/148b-3p/152-3p expression in xenograft tumors. (DOC 261 kb

A Selective and Ratiometric Bifunctional Fluorescent Probe for Al³⁺ Ion and Proton

A new bifunctional probe based on a pyrene–amino acid conjugate for the differential response of Al³⁺ and H⁺ was demonstrated for the first time. Interestingly, two solvent-dependent sensing mechanisms for Al³⁺, which feature a ratiometric change from excimer to monomer in CH₃OH and a turn-on response in water, are also disclosed

'Skaparen har inrättat de skapade ting...'

Anion to cation relay recognition was designed and realized for the first time with sequence specificity (F^–→Cu²⁺) via a fluorescence “off–on–off” mechanism. Probe <b>1</b> was a highly selective, sensitive, and turn-on chemodosimeter for F^– through a specific cyclization reaction triggered by the strong affinity of fluoride toward silicon with a significant change of fluorescence color in both ethanol and ethanol–water (1:1, v/v) solution. Fluorescence enhancement factors were dramatic: 833-fold in ethanol and 164-fold in ethanol–water (1:1, v/v) solution, respectively. The in situ system generated from the sensing of F^– showed good relay recognition ability for Cu²⁺ via fast fluorescence quenching by the formation of a 1:1 complex in ethanol–water (1:1, v/v) solution. The isolated pure compound <b>2</b> also exhibited high selectivity toward Cu²⁺ in PBS buffer (pH = 7.0) solution. The origin of this sequence specificity of fluorescence recognition was disclosed through the crystal or optimized structures and DFT calculations of corresponding compounds

A Series of Fluorescent and Colorimetric Chemodosimeters for Selective Recognition of Cyanide Based on the FRET Mechanism

A series of fluorescence “turn-on” probes (<b>PY</b>, <b>AN</b>, <b>NA</b>, <b>B1</b>, and <b>B2</b>) have been developed and successfully applied to detect cyanide anions based on the Michael addition reaction and FRET mechanism. These probes demonstrated good selectivity, high sensitivity, and very fast recognition for CN^–. In particular, the fluorescence response of probe <b>NA</b> finished within 3 s. Low limits of detection (down to 63 nM) are also obtained in these probes with remarkable fluorescence enhancement factors. In addition, fluorescence colors of these probes turned to blue, yellow, or orange upon sensing CN^–. In UV–vis mode, all of them showed ratiometric response for CN^–. ¹H NMR titration experiments and TDDFT calculations were taken to verify the mechanism of the specific reaction and fluorescence properties of the corresponding compounds. Moreover, silica gel plates with these probes were also fabricated and utilized to detect cyanide

A Series of Fluorescent and Colorimetric Chemodosimeters for Selective Recognition of Cyanide Based on the FRET Mechanism

A series of fluorescence “turn-on” probes (<b>PY</b>, <b>AN</b>, <b>NA</b>, <b>B1</b>, and <b>B2</b>) have been developed and successfully applied to detect cyanide anions based on the Michael addition reaction and FRET mechanism. These probes demonstrated good selectivity, high sensitivity, and very fast recognition for CN^–. In particular, the fluorescence response of probe <b>NA</b> finished within 3 s. Low limits of detection (down to 63 nM) are also obtained in these probes with remarkable fluorescence enhancement factors. In addition, fluorescence colors of these probes turned to blue, yellow, or orange upon sensing CN^–. In UV–vis mode, all of them showed ratiometric response for CN^–. ¹H NMR titration experiments and TDDFT calculations were taken to verify the mechanism of the specific reaction and fluorescence properties of the corresponding compounds. Moreover, silica gel plates with these probes were also fabricated and utilized to detect cyanide

Clinicopathological Significance of MicroRNA-214 in Gastric Cancer and Its Effect on Cell Biological Behaviour

<div><p>Accumulating evidence indicates that numerous microRNAs are involved in the tumorigenesis and progression of gastric cancer, while the clinical significance of microRNA-214 in gastric cancer is poorly understood and the exact role of microRNA-214 in gastric cancer remains obscure. In the present study, expression levels of microRNA-214 in 80 gastric carcinoma tissues, 18 nontumourous gastric tissues, and 4 types of gastric cancer cell lines were quantified by reverse transcription followed by real-time quantitative polymerase chain reaction (RT-qPCR), and the relationship between microRNA-214 expression and cliniopathological characteristics including prognosis was explored. To investigate the potential role of microRNA-214 in gastric cancer cell biological behaviour, we performed cell proliferation, apoptosis, migration and invasion assays in four gastric cancer cell lines and an immortalized gastric cell line <i>in vitro</i>. Our results showed that microRNA-214 was dramatically downregulated in gastric cancer tissues and gastric cancer cell lines, compared with nontumourous gastric tissues. Stepwise downregulation of microRNA-214 expression was observed among nontumourous gastric mucosa, nonmetastasis gastric cancer tissues, and metastasis gastric cancer tissues. The expression of microRNA-214 was significantly inversely correlated with lymph node metastasis and tumour size but had no correlation with the patient's prognosis. Ectopic expression of microRNA-214 could inhibit cell migration and invasion ability in SGC7901 and MKN45 gastric cancer cells. And knockdown of microRNA-214 significantly facilitated cell proliferation, migration and invasion in a cell-specific manner in MKN28, BGC823 and GES-1 cells. Colony stimulating factor 1 (CSF1) was identified as a target gene of microRNA-214. In summary, our data demonstrated that microRNA-214 is a promising novel biomarker for lymph node metastasis in patients with gastric cancer. And we identified that downregulation of microRNA-214 may regulate the proliferation, invasion and migration of gastric cancer cells by directly targeting CSF1.</p></div

A Selective, Colorimetric, and Fluorescent Chemodosimeter for Relay Recognition of Fluoride and Cyanide Anions Based on 1,1′-Binaphthyl Scaffold

The reaction-based relay recognition of fluoride and cyanide anions was demonstrated for the first time, with rapid response and unique triple (fluorescence color, intensity and absorption band) output modes in either of two sequential sensing events. Ratiometric determination of these two anions by fluorescence and/or absorbance spectra is also achieved

Effect of miR-214 on cell proliferation in SGC7901 and BGC823 cell lines using EdU assay.

<p>(A, C) Representative photographs after transfection with lentivirus miR-214-expressing vector in SGC7901 cells and miR-214 inhibitor in BGC823 cells (magnification 100×). (B, D) The percentage of EdU positive cells, calculated by EdU-labeled cell (red) number compared to total cell number (Hoechst-stained, blue), was defined as proliferation rate. The data showed no significant difference of the proliferation rate between LV3-hsa-miR-214-transfected group and the negative control group in SGC7901 cells (<i>P</i>>0.05). While we found that miR-214 inhibitor transfection could result in a strikingly increase proliferation ability of BGC823 cells (<i>P</i> = 0.0010).</p

Expression of miR-214 in different samples and its association with tumour size.

<p>(A) In comparison with 18 nontumourous gastric mucosa, miR-214 was significantly downregulated in 80 primary gastric tissues (<i>P</i> = 0.0001). MiR-214 expression was e en lower in primary gastric tissues with metastasis (Metastasis) than primary gastric tissues without metastasis (Nonmetastais). (B) Primary gastric tissues were further divided into a low-metastasis group and a high-metastasis group according to the number of lymph node metastasis. (The cutoff was set as six, which is a threshold to distinguish N0∼N1 and N2∼N3 in TNM stage (UICC, 2002). MiR-214 was dramatically reduced in high-metastasis group compared to low-metastasis group (<i>P</i> = 0.0455). (C) MiR-214 downregulation was validated in four gastric cell lines. Compared to well-moderately differentiated cell line MKN28, miR-214 was markedly attenuated in poorly differentiated cell line MKN45 and BGC823, and moderately-poorly differentiated and highly metastatic cell line SGC7901. However, we detected lower expression of miR-214 in GES-1, an immortalized gastric epithelial cell line, compared with four gastric cancer cells (^*<i>P</i><0.05). (D) Association between miR-214 expression and tumour size in primary GC was calculated by Spearman's correlation. Our data suggested that miR-214 expression was inversely correlated with tumor size (Spearman r = −0.2673, <i>P</i> = 0.0083).</p

Table_3_Prognostic and clinicopathological significance of fatty acid synthase in breast cancer: A systematic review and meta-analysis.docx

BackgroundAberrant expression of fatty acid synthase (FASN) was demonstrated in various tumors including breast cancer. A meta-analysis was conducted to investigate the role of FASN in breast cancer development and its potential prognostic significance.MethodsThe Web of Science, PubMed, Embase, and Cochrane Library databases were searched to identify studies that evaluated the relationship between FASN expression and overall survival (OS), relapse-free survival (RFS), and disease-free survival (DFS) of breast cancer patients. To analyze the clinicopathological and prognostic values of FASN expression in breast cancer, pooled hazard ratios (HRs), odds ratios (ORs), and 95% confidence intervals (CIs) were clustered based on random-effects models. To confirm whether the findings were stable and impartial, a sensitivity analysis was performed, and publication bias was estimated. Data were analyzed using Engauge Digitizer version 5.4 and Stata version 15.0.ResultsFive studies involving 855 participants were included. Patients with higher FASN expression did not have a shorter survival period compared to those with lower FASN expression (summary HR: OS, 0.73 [95% CI, 0.41-1.32; P=0.300]; DFS/RFS, 1.65 [95% CI, 0.61-4.43; P=0.323]). However, increased FASN expression was correlated with large tumor size (OR, 2.04; 95% CI, 1.04-4.00; P=0.038), higher human epidermal growth factor receptor 2 (HER2) positivity (OR, 1.53; 95% CI, 1.05-2.23; P=0.028). No significant associations were observed between FASN expression and histological grade (OR, 0.92; 95% CI, 0.41-2.04; P=0.832), Tumor Node Metastasis (TNM) stage (OR, 1.11; 95% CI, 0.49-2.53; P=0.795), nodal metastasis (OR, 1.42; 95% CI, 0.84-2.38; P=0.183), Ki-67 labelling index (OR, 0.64; 95% CI, 0.15-2.63; P=0.533), estrogen receptor (ER) status (OR, 0.90; 95% CI, 0.61-1.32; P=0.586), or progesterone receptor (PR) status (OR, 0.67; 95% CI, 0.29-1.56; P=0.354).ConclusionFASN is associated with HER2 expression and may contribute to tumor growth, but it has no significant impact on the overall prognosis of breast cancer.</p