NERVE CONDUCTION VELOCITY INVESTIGATION IN ATHLETES WITH TRAINED LOWER EXTRAMITY FOR WELL·CONTROLLlNG MOVEMENT

Reaction, coordination and speed ability are essential for success in a variety of sports. Nerve conduction velocity (NCV) is related to all of these abilities. Previous studies had investigated power and endurance type athletes, but there is less research in athletes who are skilled in coordinated lower extremity movements requiring more neural adaptation for nerve conduction velocity after specific exercise training. The purpose of this study is to investigate nerve conduction velocity of soccer, sanshou players and untrained subjects. Results show that NCV was significantly different among the 3 groups (p < .05) for the femoral and tibal nerves. For both femoral and tibial nerve.§, results showed that the soccer players had significantly faster NCV than the other groups (p < .05). According to the results, faster NCV adaptation from long term training in soccer players may be relate to their movement requirement of changing their movement direction quickly and skillfully

POOR FORCE CONTROL PERFORMANCE AND UNVARIED EMG PATTERN AFTER AGONIST MUSCLE FATIGUE IN HUMANS

Ballistic and accurately control of a targeted fast contraction relies on phasic activations of the agonist and antagonist muscles. The purpose of the study was to investigate the effect of tibialis anterior (TA) fatigue on the systematic bias and the consistence of the net dorsiflexion torque generation and the controlling pattern of the agonist-antagonist muscles. Ten subjects were tested twice with a week apart. Fast and slow dorsiflexion at 40%-MVG were measured before and after fatigue of the TA by voluntary isometric dorsiflexions. The EMG of the TA and soleus (Sol) were recorded. The results revealed that more post-fatigue increment of the systematic error was in the fast dorsiflexions, random error increment were similar in both speeds of isometric dorsiflexions, the cocontraction ratio increased after fatigue only in the slow dorsiflexions. Our results suggested that precision of the fast targeted isometric contractions was reduced after fatigue because of unvaried agonist-antagonist control strategy

Subject-relevant Document Recommendation: A Reference Topic-Based Approach

Knowledge-intensive workers, such as academic researchers, medical professionals or patent engineers, have a demanding need of searching information relevant to their work. Content-based recommender system (CBRS) makes recommendation by analyzing similarity of textual contents between documents and users’ preferences. Although content-based filtering has been one of the promising approaches to document recommendations, it encounters the over-specialization problem. CBRS tends to recommend documents that are similar to what have been in user’s preference profile. Rationally, citations in an article represent the intellectual/affective balance of the individual interpretation in time and domain understanding. A cited article shall be associated with and may reflect the subject domain of its citing articles. Our study addresses the over-specialization problem to support the information needs of researchers. We propose a Reference Topic-based Document Recommendation (RTDR) technique, which exploits the citation information of a focal user’s preferred documents and thereby recommends documents that are relevant to the subject domain of his or her preference. Our primary evaluation results suggest the outperformance of the proposed RTDR to the benchmarks

Pathophysiology of Neuropathic Pain in Type 2 Diabetes: Skin denervation and contact heat–evoked potentials

OBJECTIVE: Neuropathic pain due to small-fiber sensory neuropathy in type 2 diabetes can be diagnosed by skin biopsy with quantification of intra- epidermal nerve fiber ( IENF) density. There is, however, a lack of noninvasive physiological assessment. Contact heat-evoked potential ( CHEP ) is a newly developed approach to record cerebral responses of A fiber- mediated thermonociceptive stimuli. We investigated the diagnostic role of CHEP. RESEARCH DESIGN AND METHODS: From 2006 to 2009, there were 32 type 2 diabetic patients (20 males and 12 females, aged 51.63 10.93 years) with skin denervation and neuropathic pain. CHEPs were recorded with heat stimulations at the distal leg, where skin biopsy was performed. RESULTS: CHEP amplitude was reduced in patients compared with age- and sex-matched control subjects (14.8 15.6 vs. 33.7 10.1 V, P < 0.001). Abnormal CHEP patterns ( reduced amplitude or prolonged latency) were noted in 81.3 % of these patients. The CHEP amplitude was the most significant parameter correlated with IENF density (P = 0. 003) and pain perception to contact heat stimuli (P = 0.019) on multiple linear regression models. An excitability index was derived by calculating the ratio of the CHEP amplitude over the IENF density. This excitability index was higher in diabetic patients than in control subjects (P = 0.023), indicating enhanced brain activities in neuropathic pain. Among different neuropathic pain symptoms, the subgroup with evoked pain had higher CHEP amplitudes than the subgroup without evoked pain (P = 0.011). CONCLUSIONS: CHEP offers a noninvasive approach to evaluate the degeneration of thermonociceptive nerves in diabetic neuropathy by providing physiological correlates of skin denervation and neuropathic pain

Evaluation of Lentiviral-Mediated Expression of Sodium Iodide Symporter in Anaplastic Thyroid Cancer and the Efficacy of In Vivo Imaging and Therapy

Anaplastic thyroid carcinoma (ATC) is one of the most deadly cancers. With intensive multimodalities of treatment, the survival remains low. ATC is not sensitive to 131I therapy due to loss of sodium iodide symporter (NIS) gene expression. We have previously generated a stable human NIS-expressing ATC cell line, ARO, and the ability of iodide accumulation was restored. To make NIS-mediated gene therapy more applicable, this study aimed to establish a lentiviral system for transferring hNIS gene to cells and to evaluate the efficacy of in vitro and in vivo radioiodide accumulation for imaging and therapy. Lentivirus containing hNIS cDNA were produced to transduce ARO cells which do not concentrate iodide. Gene expression, cell function, radioiodide imaging and treatment were evaluated in vitro and in vivo. Results showed that the transduced cells were restored to express hNIS and accumulated higher amount of radioiodide than parental cells. Therapeutic dose of 131I effectively inhibited the tumor growth derived from transduced cells as compared to saline-treated mice. Our results suggest that the lentiviral system efficiently transferred and expressed hNIS gene in ATC cells. The transduced cells showed a promising result of tumor imaging and therapy

Lack of association of genetic variants for diabetic retinopathy in Taiwanese patients with diabetic nephropathy

[[abstract]]Objective Diabetic nephropathy (DN) and diabetic retinopathy (DR) comprise major microvascular complications of diabetes that occur with a high concordance rate in patients and are considered to potentially share pathogeneses. In this case-control study, we sought to investigate whether DR-related single nucleotide polymorphisms (SNPs) exert pleiotropic effects on renal function outcomes among patients with diabetes. Research design and methods A total of 33 DR-related SNPs were identified by replicating published SNPs and via a genome-wide association study. Furthermore, we assessed the cumulative effects by creating a weighted genetic risk score and evaluated the discriminatory and prediction ability of these genetic variants using DN cases according to estimated glomerular filtration rate (eGFR) status along with a cohort with early renal functional decline (ERFD). Results Multivariate logistic regression models revealed that the DR-related SNPs afforded no individual or cumulative genetic effect on the nephropathy risk, eGFR status or ERFD outcome among patients with type two diabetes in Taiwan. Conclusion Our findings indicate that larger studies would be necessary to clearly ascertain the effects of individual genetic variants and further investigation is also required to identify other genetic pathways underlying DN.[[notice]]補正完

Crystallinity Improvement of ZnO Thin Film on Different Buffer Layers Grown by MBE

The material and optical properties of ZnO thin film samples grown on different buffer layers on sapphire substrates through a two-step temperature variation growth by molecular beam epitaxy were investigated. The thin buffer layer between the ZnO layer and the sapphire substrate decreased the lattice mismatch to achieve higher quality ZnO thin film growth. A GaN buffer layer slightly increased the quality of the ZnO thin film, but the threading dislocations still stretched along the c-axis of the GaN layer. The use of MgO as the buffer layer decreased the surface roughness of the ZnO thin film by 58.8% due to the suppression of surface cracks through strain transfer of the sample. From deep level emission and rocking curve measurements it was found that the threading dislocations play a more important role than oxygen vacancies for high-quality ZnO thin film growth

Transcriptome Analysis of Synaptoneurosomes Identifies Neuroplasticity Genes Overexpressed in Incipient Alzheimer's Disease

In Alzheimer's disease (AD), early deficits in learning and memory are a consequence of synaptic modification induced by toxic beta-amyloid oligomers (oAβ). To identify immediate molecular targets downstream of oAβ binding, we prepared synaptoneurosomes from prefrontal cortex of control and incipient AD (IAD) patients, and isolated mRNAs for comparison of gene expression. This novel approach concentrates synaptic mRNA, thereby increasing the ratio of synaptic to somal mRNA and allowing discrimination of expression changes in synaptically localized genes. In IAD patients, global measures of cognition declined with increasing levels of dimeric Aβ (dAβ). These patients also showed increased expression of neuroplasticity related genes, many encoding 3′UTR consensus sequences that regulate translation in the synapse. An increase in mRNA encoding the GluR2 subunit of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) was paralleled by elevated expression of the corresponding protein in IAD. These results imply a functional impact on synaptic transmission as GluR2, if inserted, maintains the receptors in a low conductance state. Some overexpressed genes may induce early deficits in cognition and others compensatory mechanisms, providing targets for intervention to moderate the response to dAβ