1 research outputs found
Simultaneous Surface-Enhanced Raman Spectroscopy Detection of Multiplexed MicroRNA Biomarkers
Simultaneous detection
of cancer biomarkers holds great promise
for the early diagnosis of cancer. In the present work, an ultrasensitive
and reliable surface-enhanced Raman scattering (SERS) sensor has been
developed for simultaneous detection of multiple liver cancer related
microRNA (miRNA) biomarkers. We first proposed a novel strategy for
the synthesis of nanogap-based SERS nanotags by modifying gold nanoparticles
(AuNPs) with thiolated DNA and nonfluorescent small encoding molecules.
We also explored a simple approach to a green synthesis of hollow
silver microspheres (Ag-HMSs) with bacteria as templates. On the basis
of the sandwich hybridization assay, probe DNA-conjugated SERS nanotags
used as SERS nanoprobes and capture DNA-conjugated Ag-HMSs used as
capture substrates were developed for the detection of target miRNA
with a detection limit of 10 fM. Multiplexing capability for simultaneous
detection of the three liver cancer related miRNAs with the high sensitivity
and specificity was demonstrated using the proposed SERS sensor. Furthermore,
the practicability of the SERS sensor was supported by the successful
determination of target miRNA in cancer cells. The experimental results
indicated that the proposed strategy holds significant potential for
multiplex detection of cancer biomarkers and offers the opportunity
for future applications in clinical diagnosis