298 research outputs found

    Efeito da adubação mineral sobre a produção de frutos em pupunheira sem espinho (Bactris gasipaes Kunth).

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    O trabalho tem como objetivo avaliar a produção de pupunheira sem espinho em diferentes adubações

    Programa de melhoramento genético de pupunha na Embrapa, IAC e INPA.

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    Estado da arte; Recursos genéticos; Estratégias de melhoramento; Desenvolvimento experimental.bitstream/item/30975/1/Doc205.pd

    Contribution of the institutions in the Northern region of Brazil to the development of plant cultivars and their impact on agriculture.

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    This paper describes the development of breeding programs in northern Brazil and their main impacts on agriculture. Their contribution to the breeding of the species palm oil, acai fruit, cacao, cupuaçu, guarana, tomato, camu-camu, cocona, peach palm, and rubber was laid out in detail. Advances in breeding programs of institutions such as Embrapa, Ceplac, Inpa, and Universities require investments in infrastructure and in human and financial resources to ensure continuity and efficiency in economic, social and environmental gains. The improvement of native species, the main focus of the breeding programs of the institutions in the Northern region of Brazil, is a form of exploiting the Amazonian biodiversity for the benefit of society. Therefore, policies to foster research institutions should be a subject of deliberation and action of the scientific and technological community in Brazil

    Glucocerebrosidases catalyze a transgalactosylation reaction that yields a newly-identified brain sterol metabolite, galactosylated cholesterol

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    ?-Glucocerebrosidase (GBA) hydrolyzes glucosylceramide (GlcCer) to generate ceramide. Previously, we demonstrated that lysosomal GBA1 and nonlysosomal GBA2 possess not only GlcCer hydrolase activity, but also transglucosylation activity to transfer the glucose residue from GlcCer to cholesterol to form ?-cholesterylglucoside (?-GlcChol) in vitro. ?-GlcChol is a member of sterylglycosides present in diverse species. How GBA1 and GBA2 mediate ?-GlcChol metabolism in the brain is unknown. Here, we purified and characterized sterylglycosides from rodent and fish brains. Although glucose is thought to be the sole carbohydrate component of sterylglycosides in vertebrates, structural analysis of rat brain sterylglycosides revealed the presence of galactosylated cholesterol (?-GalChol), in addition to ?-GlcChol. Analyses of brain tissues from GBA2-deficient mice and GBA1- and/or GBA2-deficient Japanese rice fish (Oryzias latipes) revealed that GBA1 and GBA2 are responsible for ?-GlcChol degradation and formation, respectively, and that both GBA1 and GBA2 are responsible for ?-GalChol formation. Liquid chromatography?tandem MS revealed that ?-GlcChol and ?-GalChol are present throughout development from embryo to adult in the mouse brain. We found that ?-GalChol expression depends on galactosylceramide (GalCer), and developmental onset of ?-GalChol biosynthesis appeared to be during myelination. We also found that ?-GlcChol and ?-GalChol are secreted from neurons and glial cells in association with exosomes. In vitro enzyme assays confirmed that GBA1 and GBA2 have transgalactosylation activity to transfer the galactose residue from GalCer to cholesterol to form ?-GalChol. This is the first report of the existence of ?-GalChol in vertebrates and how ?-GlcChol and ?-GalChol are formed in the brain.Medical Biochemistr
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