Mutasi Structural Intron Trnl (Uaa) Pada Suku Meranti-merantian (Dipterocarpaceae) [Structural Mutation of Trnl Intron (Uaa) in Dipterocarpaceae]

TRNA " UAA (trnL) intron have been widely utilised for phylogenetic reconstruction in angiosperm families. Two main helical elements of secondary structure of trnL intron (stem loop regions P6 and P8) contained the most variable sequences across taxa.Detailed examination on the insertion-deletion events in trnL intron sequences of 110 species of Dipterocarpaceae has identified 4 stem loop structures within both loops.The first stemJoqp was found in P6 loop and three other were discovered in P8 loop.These structures featuring characteristic for genericJinfra-generic level depended upon which taxonomic classifications are foJJowed.One of these structures formed a large loop of 72 nucleotides and was a unique major stem loop in Dipterocarps family whose loop was the major structural mutation of trnL intron in this family

Keragaman Genetik Beberapa Klon Durian (Durio Zibethinus Murray) Asal Jawa Barat Berdasarkan Sidik Random Amplified Polimorphic Dna [Genetic Diversity of Durian (Durio Zibethinus Murray) From West Java Based on Random Amplified Polymorphic Dna Fingerprint]

Durian (Durio zibethinus) is one of the most popular tropical fruits in SE Asia. Indonesia has several local clones that have not yet been widely introduced to local fruit markets. This present study aimed to assess genetic diversity of 17 clones of durian from West Java based on RAPD fingerprints. Thirty RAPD's primers were initially screened and four were selected for the analysis.These four primers (OPA 13, OPD 8, OPN 6 and OPA 18) generated 63 scorable bands to which 100% of them were polymorphic.OPA-13 at 700bp was exclusively possessed by Tambleg clone and other bands were shared among the other clones. Clustering analysis was performed based on RAPD profiles using the UPGMA method. The range of genetic similarity value among genotypes was 0.15-0.73 suggesting high genetic variation among them. Results from genetic diversity analysis based on plant propagation system showed a higher genetic diversity value in occulating (87.30%) plants than that of grafting (60.32%)

Keragaman Fenotipe Rapd Santalum Album L. Dipulau Timor Bagian Timur [Rapd Phenotypic Variation of Santalum Album L. in Eastern Part of Timor]

Santalum album L. (sandalwood/cendana) is known as one of medicinal and aromatic tree species in Indonesia. The species is valued for its quality light wood timber and for its medicinal properties.The species has been overexploited and is considered as vulnerable plant species.The present study aimed to assess genetic diversity and to estimate genetic relationship among 58 accessions of plant germplasm collection using Random Amplified Polymorphic DNA (RAPD).Two RAPD primers generated 34 scorable bands with 97.06% of them were polymorphic. Clustering analysis was performed based on RAPD profiles using the UPGMA method.The range of genetic dissimilarity value among species was from 6% to 91%, while the range of genetic distance between populations was from 1.89% and 26.88%.These values showed that 5.album from Eastern part of Timor was genetically diverse populations.Within the 12 populations, there were 9 banding patterns recorded from primer OPA 16 and 12 banding patterns from primer OPB 12, suggesting that OPB 12 was more sensitive than that of OPA 16 to show variation within the sample used

Modified Step Variational Iteration Method for Solving Fractional Biochemical Reaction Model

A new method called the modification of step variational iteration method (MoSVIM) is introduced and used to solve the fractional biochemical reaction model. The MoSVIM uses general Lagrange multipliers for construction of the correction functional for the problems, and it runs by step approach, which is to divide the interval into subintervals with time step, and the solutions are obtained at each subinterval as well adopting a nonzero auxiliary parameter ℏ to control the convergence region of series' solutions. The MoSVIM yields an analytical solution of a rapidly convergent infinite power series with easily computable terms and produces a good approximate solution on enlarged intervals for solving the fractional biochemical reaction model. The accuracy of the results obtained is in a excellent agreement with the Adam Bashforth Moulton method (ABMM)

Deteksi Mutan Kentang Hitam Hasil Radiasi Sinar Γ Menggunakan Marka ISSR Dan RAPD

Umbi kentang hitam [Plectranthus rotundifolius (Poir.) Spreng.] merupakan salah satu sumber pangan alternatif bagi sebagian masyarakat Indonesia. Namun, rendahnya keragaman genetik kentang hitam menjadi kendala dalam perakitan varietas unggul. Pemuliaan tanaman dengan cara mutasi antara lain dengan iradiasi sinar γ diharapkan dapat meningkatkan keragaman genetik kentang hitam. Penelitian dilaksanakan pada Bulan Januari - November 2010 di Laboratorium Genetika Tumbuhan, PusatPenelitian Biologi LIPI. Penelitian ini bertujuan mendeteksi mutan kentang hitam hasil radiasi sinar γ pada dosis 6, 25, dan 35 gray dengan menggunakan marka ISSR dan RAPD. Lima belas primer ISSR dan RAPD digunakan untuk mengamplifikasi genom DNA total. Di antara ke-15 primer tersebut, hanya empat primer yang menghasilkan pita-pita polimorfik untuk mendeteksi mutan hasil mutasi pada konsentrasi 25 gray yaitu (OPA 13, OPA 18, OPB 18, dan UBC 834) pada aksesi 1#10; 11.3#4; 11.3#5, dan 2.10#8 dan hanya tiga primer yang mampu mendeteksi mutan pada konsentrasi 35 gray (OPA 13, OPB 18, dan UBC 807) pada aksesi 4.10#2, 4.10#1, 4.10#4, 4.10#3, 4.10#5, dan 33d.1#3. Hal ini menunjukkan bahwa marka ISSR dan RAPD dapat digunakan untuk mendeteksi mutan pada kentang hitam

Analisis Keragaman Genetik Kentang Hitam [Plectranthus Rotundifolius (Poiret) Sprengel] Berdasarkan Marka Issr Dan Rapd. [Analyisis of Genetic Variations of 'Kentang Hitam' Plectranthus Rotundifolius (Poiret) Sprengel Based on Issr and Rapd Marker]

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions