ヒロシマ ダイガク シガクブ リンショウ ジッシュウ シサツ ホウコク

We made a field trip to Hiroshima University Hospital to observe its clinical training practices on February 22, 2013. Distinctive features of the clinical training system were as follows. 1) Training schedule. Learning objectives and clinical cases required are established in each specialty clinics. Students can make training schedules in each specialty clinic for themselves in accordance with the progress of required cases of their own. They can move from a clinic to other clinic in a day in order to follow their cases. 2) The small group system and the tutor system for the group. Students are assigned to a group consisting of 3 to 4 persons. They get a clinical training together and share clinical experiences with each other. They hold the group meeting every other Wednesday. The tutor checks the progress on their cases and discusses about next training objectives with each person. 3) The stepwise training and evaluation system. The clinical training are given in 3 terms. Learning objectives in each term are established in stepwise setting. Students are evaluated for their levels of attainment at the end of each term. 4) Morning meeting and mini-lecture. Students attend morning meeting and a mini-lecture before the clinical training everyday. 5) Clinical clerkship. Clinical clerkship has been performed with support of patients. Patients in the hospital are informed for the clinical clerkship. Participants provide written informed consent prior to entry into the system. However, the number of patients for clinical clerkship were not enough. These features are informative and helpful for us to improve our clinical training system. And recruitment of patients who kindly support the clinical clerkship may be the biggest problem to solve in both universities

ニホン ダイガク マツド シガクブ リンショウ ジッシュウ シサツ ホウコク

Clinical training inspection at Nihon University School of Dentistry at Matsudo was carried out on February 15, 2013, and useful information for clinical training was collected. The clinical training begins in April of the fifth grade, and it ends in June of the sixth grade. The patients are handed over from sixth- to fifth-year students in pair-policlinic training, which is carried out in the first month of clinical training. The number of patients is approximately 20 per student. Objective structured clinical achievement tests (OSCAT) are conducted at a later stage of the fifth grade, and subjects of OSCAT perform a medical interview, composite resin filling, root canal therapy, SRP, muscle trimming, tooth extraction, and vital sign checks. OSCAT is carried out in the testing time from 4 to 20 minutes. Subjects which are difficult to evaluate with such a practical test such as case analysis and treatment strategy development are tested by the written examinations. We are discussing the adoption of OSCAT at the end of the clinical training in our university, serving as a useful and helpful reference. The clinical training is completed by June of the sixth grade, and then classroom lectures are started to prepare for the graduation examination with multiple choice questions (MCQ) in December

トウキョウ イカ シカ ダイガク シガクブ シガッカ ノ リンショウ ジッシュウ シサツ ホウコク

In recent years, dental services are subdivided and complicated, moreover the social circumstances change busily. An important object of the dental education is to bring up the dentist who had rich knowledge and rich human nature adaptable to such a change. The dental students can learn knowledge and skills through lectures and phantom practices. However, it is essential to experience dental examination and treatment in the clinical field to bring up dentists such as the above dentists. In the University of Tokushima faculty of dentistry, clinical clerkship has been performed by patients' cooperation, and university students have learned a communicative competence and behavior to contact with the patients as well as the knowledge and skill of dental treatment, and improved the professional ethics. On the other hand, the patients suffering from underlying disease except dental disease such as hypertension, diabetes mellitus and heart disorder increase. When these diseases are particularly serious, scrupulous attention is necessary in the dental treatment, these patients are unsuitable for clinical clerkship if the patients are cooperative. Moreover it becomes difficult to get the patients' cooperation for clinical clerkship year by year. The improvement of dental clinical education is a matter of great urgency corresponding to such situation. Therefore we inspected a clinical clerkship in faculty of dentistry, Tokyo Medical and Dental University and collected information for improvement of clinical clerkship of the University of Tokushima, faculty of dentistry

Common Peak Approach Using Mass Spectrometry Data Sets for Predicting the Effects of Anticancer Drugs on Breast Cancer

We propose a method for biomarker discovery from mass spectrometry data, improving the common peak approach developed by Fushiki et al. (BMC Bioinformatics, 7:358, 2006). The common peak method is a simple way to select the sensible peaks that are shared with many subjects among all detected peaks by combining a standard spectrum alignment and kernel density estimates. The key idea of our proposed method is to apply the common peak approach to each class label separately. Hence, the proposed method gains more informative peaks for predicting class labels, while minor peaks associated with specific subjects are deleted correctly. We used a SELDI-TOF MS data set from laser microdissected cancer tissues for predicting the treatment effects of neoadjuvant therapy using an anticancer drug on breast cancer patients. The AdaBoost algorithm is adopted for pattern recognition, based on the set of candidate peaks selected by the proposed method. The analysis gives good performance in the sense of test errors for classifying the class labels for a given feature vector of selected peak values

Cholesterol Efflux Capacity of Apolipoprotein A-I Varies with the Extent of Differentiation and Foam Cell Formation of THP-1 Cells

Apolipoprotein A-I (apoA-I), the main protein component of high-density lipoprotein (HDL), has many protective functions against atherosclerosis, one of them being cholesterol efflux capacity. Although cholesterol efflux capacity measurement is suggested to be a key biomarker for evaluating the risk of development of atherosclerosis, the assay has not been optimized till date. This study aims at investigating the effect of different states of cells on the cholesterol efflux capacity. We also studied the effect of apoA-I modification by homocysteine, a risk factor for atherosclerosis, on cholesterol efflux capacity in different states of cells. The cholesterol efflux capacity of apoA-I was greatly influenced by the extent of differentiation of THP-1 cells and attenuated by excessive foam cell formation. N-Homocysteinylated apoA-I indicated a lower cholesterol efflux capacity than normal apoA-I in the optimized condition, whereas no significant difference was observed in the cholesterol efflux capacity between apoA-I in the excessive cell differentiation or foam cell formation states. These results suggest that cholesterol efflux capacity of apoA-I varies depending on the state of cells. Therefore, the cholesterol efflux assay should be performed using protocols optimized according to the objective of the experiment

ニホン シカ ダイガク リンショウ ジッシュウ シサツ ホウコク

We made a field trip to Nippon Dental University Hospital to observe its clinical training practices on February 16, 2011. Distinctive features of the clinical training system were as follows. 1) Clinical training is given to fifth-year students. Students in the sixth-year are intent on their studies. 2) There is a one-month overlap in the training periods between these two groups, as patients are handed over from the fifth- to the fourth-year students. This handover is a student-led event. 3) After this period, pre-clinical training is given to the fifth-year students for three months, and then clinical training begins. 4) Patients are requested to evaluate and give feedback to the students treating them. Each student should receive five or more evaluations. 5) A mentor system was introduced in 2005. According to our findings and the results of the“ World Café” held on the same day with trainee dentists and fifth-year students, we identified the following means of improving our clinical training system. First, students under clinical training are now permitted to participate in case-report conferences of trainee dentists, as of 2011. This program was introduced based on the concept of top-down processing to help students form perceptions about cases and treatment. Second, the necessity of a faculty-development program focused on clinical training is recognized, to standardize and improve the guidance given to students by advising doctors. Third, the mentor system and the“ World Café” need more attention as elements of clinical training to support students and motivate improvement

FUNCTION OF miR-494-3p IN OSTEOBLASTS

Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3'-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation in osteoblasts by up-regulating miR-494-3p followed by FGFR2 and ROCK1 gene repressions

Aprepitant plus granisetron and dexamethasone for prevention of chemotherapy-induced nausea and vomiting in patients with gastric cancer treated with S-1 plus cisplatin

Background: We aimed to evaluate the efficacy of a new combination antiemetic therapy comprising aprepitant, granisetron, and dexamethasone in gastric cancer patients undergoing chemotherapy with cisplatin and S-1. Methods: Gastric cancer patients scheduled to receive their first course of chemotherapy with cisplatin (60 mg/m2) and S-1 (80 mg/m2) were treated with a new combination antiemetic therapy aprepitant, granisetron, and dexamethasone on day 1; aprepitant and dexamethasone on days 2 and 3; and dexamethasone on day 4. The patients reported vomiting, nausea, use of rescue therapy, and change in the amount of diet intake, and completed the Functional Living Index-Emesis (FLIE) questionnaire. The primary endpoint was complete response (CR; no emesis and use of no rescue antiemetics) during the overall study phase (0-120 h after cisplatin administration). The secondary endpoints included complete protection (CP; CR plus no significant nausea); change in the amount of diet intake; and the impact of chemotherapy-induced nausea and vomiting (CINV) on daily life during the overall, acute (0-24 h), and delayed (24-120 h) phases. Results: Fifty-three patients were included. CR was achieved in 88.7, 98.1, and 88.7 % of patients in the overall, acute, and delayed phases, respectively. The corresponding rates of CP were 67.9, 96.2, and 67.9 %. Approximately half of the patients had some degree of anorexia. FLIE results indicated that 79.5 % of patients reported "minimal or no impact of CINV on daily life". Conclusions: Addition of aprepitant to standard antiemetic therapy was effective in gastric cancer patients undergoing treatment with cisplatin and S-1. © 2013 Springer Japan

Susceptibility of muridae cell lines to ecotropic murine leukemia virus and the cationic amino acid transporter 1 viral receptor sequences: implications for evolution of the viral receptor

Ecotropic murine leukemia viruses (Eco-MLVs) infect mouse and rat, but not other mammalian cells, and gain access for infection through binding the cationic amino acid transporter 1 (CAT1). Glycosylation of the rat and hamster CAT1s inhibits Eco-MLV infection, and treatment of rat and hamster cells with a glycosylation inhibitor, tunicamycin, enhances Eco-MLV infection. Although the mouse CAT1 is also glycosylated, it does not inhibit Eco-MLV infection. Comparison of amino acid sequences between the rat and mouse CAT1s shows amino acid insertions in the rat protein near the Eco-MLV-binding motif. In addition to the insertion present in the rat CAT1, the hamster CAT1 has additional amino acid insertions. In contrast, tunicamycin treatment of mink and human cells does not elevate the infection, because their CAT1s do not have the Eco-MLV-binding motif. To define the evolutionary pathway of the Eco-MLV receptor, we analyzed CAT1 sequences and susceptibility to Eco-MLV infection of other several murinae animals, including the southern vole (Microtus rossiaemeridionalis), large Japanese field mouse (Apodemus speciosus), and Eurasian harvest mouse ( Micromys minutus). Eco-MLV infection was enhanced by tunicamycin in these cells, and their CAT1 sequences have the insertions like the hamster CAT1. Phylogenetic analysis of mammalian CAT1s suggested that the ancestral CAT1 does not have the Eco-MLV-binding motif, like the human CAT1, and the mouse CAT1 is thought to be generated by the amino acid deletions in the third extracellular loop of CAT1

Increased E-selectin in hepatic ischemia-reperfusion injury mediates liver metastasis of pancreatic cancer

金沢大学医薬保健研究域医学系Several recent studies have reported that selectins are produced during ischemia-reperfusion injury, and that selectin ligands play an important role in cell binding to the endothelium and in liver metastasis. Portal clamping during pancreaticoduodenectomy with vessel resection for pancreatic head cancer causes hepatic ischemia-reperfusion injury, which might promote liver metastasis. We investigated the liver colonization of pancreatic cancer cells under hepatic ischemia-reperfusion and examined the involvement of E-selectin and its ligands. A human pancreatic cancer cell line (Capan-1) was injected into the spleen of mice after hepatic ischemia-reperfusion (I/R group). In addition, to investigate the effect of an anti-E-selectin antibody on liver colonization in the IR group, mice received an intraperitoneal injection of the anti-E-selectin antibody following hepatic ischemia-reperfusion and tumor inoculation (IR+Ab group). Four weeks later, mice were sacrificed and the number of tumor nodules on the liver was compared to mice without hepatic ischemia-reperfusion (control group). The incidence of liver metastasis in the I/R group was significantly higher (16 of 20, 80%) than that in the control group (6 of 20, 30%) (P<0.01). Moreover, mice in the I/R group had significantly more tumor nodules compared to those in the control group (median, 9.9 vs. 2.7 nodules) (P<0.01). In the I/R+Ab group, only 2 of 5 (40%) mice developed liver metastases. RT-PCR and southern blotting of the liver extracts showed that the expression of IL-1 and E-selectin mRNA after hepatic ischemia-reperfusion was significantly higher than the basal levels. Hepatic ischemia-reperfusion increases liver metastases and E-selectin expression in pancreatic cancer. These results suggest that E-selectin produced due to hepatic ischemia-reperfusion is involved in liver metastasis.Embargo Period 6 month