Dose-dependent effects of L-carnitine on blood sialic acid, MDA and GSH concentrations in BALB/c mice

L-carnitine is an essential quarternary amine having an important role in the P-oxidation of fatty acids. Although L-carnitine was shown to be protective against toxic effects of some chemicals the dose-effect relationship with respect to its antioxidant action and protection from lipid peroxidation is unknown. To evaluate the dose-response profile of L-carnitine on blood sialic acid, glutathione and malondialdehyde concentrations, 40 mice were randomly allocated to 4 groups. Experimental mice were treated with intraperitoneal saline for 5 days (Group 1), L-carnitine at 100 mg/kg for 5 days (Group 2), L-carnitine at 250 mg/kg for 5 days (Group 3), L-carnitine at 500 mg/kg for 5 days (Group 4). Following the treatments, blood samples were collected, and blood glutathione, malondialdehyde and sialic acid concentrations were determined. L-carnitine provided an antioxidant action at doses of 100, 250 and 500 mg/kg with the strongest antioxidant action observed at 500 mg/kg dose. There was a significant increase in malondialdehyde and sialic acid concentrations at all doses of L-carnitine with the highest effect seen at 500 mg/kg dose. In addition, L-carnitine caused a dose-dependent elevation in glutathione level

Effects of L-carnitine on kidney histopathology, plasma and tissue total sialic acid, malondialdehyde and glutathione concentrations in response to gentamicin administration in Balb/C mice

L-carnitine is an essential cofactor in mitochondrial oxidation of fatty acids with antioxidant and protective effects against lipid peroxidation. We investigated the protective effect of L-carnitine on gentamicin nephrotoxicity and the changes of plasma and kidney total sialic acid concentrations with gentamicin treatments. Twenty four Balb/C mice were divided into 4 groups. Group 1 received subcutaneous (s.c.) isotonic saline daily for 5 days. Group 2 was daily treated with s.c. injection of 500 mg/kg L-Carnitine for 5 days, group 3 was treated with 100 mg/kg of gentamicin (s.c.) injection daily for 5 days, and group 4 with 500 mg/kg L-Carnitine (s.c.) plus 100 mg/kg (s.c.) gentamicin for 5 days. Plasma and tissue glutathione (GSH), malondialdehyde (MDA) and total sialic acid (TSA) concentrations were measured on day 5. Blood urea and creatinine as well as kidney histopathology were also evaluated to assess the nephrotoxic effect of gentamicin. Administration of L-carnitine significantly reduced the gentamicin-induced increases of MDA and TSA concentrations in the plasma and kidneys. In addition. L-carnitine reduced the gentamicin-nephrotoxicity as evidenced by blood urea, creatinine and kidney histopathology. L-carnitine also improved the antioxidant status in the plasma and kidneys of gentamicin treated mice by significantly increasing GSH concentration compared to controls and to mice treated with gentamicin alone. These results suggest that L-carnitine may attenuate gentamicin-induced nephrotoxicity by improving antioxidant status, and reducing tissular lesions in Balb/C mice