PROSTATE-SPECIFIC ANTIGEN A Clue for the Prostatic Origin of Metastasis

The prostate-specific antigen is a recently purified glycoprotein which is present only in the prostatic gland. In order to confirm the usefulness of this protein in isolating prostatic carcinomas from socalled metastatic carcinomas of unknown primary site, we immunohistochemically studied 19 non-neoplastic prostatic tissue, 18 primary carcinomas of the prostate, and 32 non-prostatic adenocarcinomas. From our study, we concluded that PSA is highly specific for the prostatic carcinomas. The absence of PSA, however, does not necessarily rule out its prostatic origin when the tumor is poorly differentiated. In our laboratory, from now on, PSA will be applied routinely in cases of metastatic carcinomas of unknown origin

A Study of Lecture Meetings and Childcare Activities in The Kindergarten

本研究の目的は、子育て支援の一環として試みた講演会や親子での活動が保護者にどのように受け止められ活動の後で変化をもたらしたかをアンケート調査し、今後の講演会や親子活動のあり方を考える上での基礎資料を得ることである。その結果、親子が一緒に活動するものや内容が具体的で保護者のニーズに対応したものの方が、参加率が高く、男女共7割以上の保護者が参加する前より考え方を変化させている。そして、親子参加型の子育て支援が望まれていることが明らかになった。The purpose of this study was to examine the effect of our support of childcare activities: lecture meetings and activities with parents and children. Parents were asked how they accepted these activities and what changes were brought by them. The results should that many parents took part in activities with children, and lecture meetings which had practical and concrete contents. And more than 70% of parents have changed their way of thinking

A THREE-DIMENSIONAL MOTION ANALYSIS OF TWO-HANDED AND WAIST BELT PULLING BACKWARD EXERCISES IN ELITE TUG OF WAR ATHLETES

In order to find the benefits of the waist belt (WB) in Tug of War (TOW) sport, the purpose of this study was to compare kinematic differences between two-handed (TH) and WB pulling backward exercises. The team that holds the gold medal record for the World Indoor TOW Championships 2004 participated in this study (N=20). According to threedimensional video analysis procedures using the direct linear transformation analysis method, the mean body center of mass (CaM) displacement during TH and WB trials were 0.7m and 1.45m, respectively. Moreover, the mean CaM speed of WB was approximately 1.6 times faster than that of TH. These results suggest that the WB had the efficacy to accomplish a given task in the pulling backward exercise. Therefore it is concluded that WB might be one of useful equipmenls in the TOW sport

Immunohistologic Analysis of Malignant Lymphoma 1. Accuracy of Histological Diagnosis and Usefulness of Monoclonal Antibodies

In order to test whether B cell or T cell origin of malignant lymphomas can be reliably suspected on histological basis alone, and to know the usefulness of some antibodies to lymphocytic cells which are currently available commercially, we reviewed 30 cases of malignant lymphomas both histologically and immunohistochemically. Hematoxylin- eosin stained sections from those cases were reclassified using Lukes and Collins\u27 classification with minor modification. Then, monoclonal antibodies; LCA, MT-1, and MB-1 have been applied to paraffin sections from same cases. In addition, four cases of plasmacytoma and a case of metastatic carcinoma which resembled malignant lymphoma were studied immunohistochemically. Our results indicate that (1) in most cases, T and B cell origin of lymphoma may be accurately classified by H-E stainecd section although further study with more cases is definitely necessary to reach this conclusion. (2) LCA immunoreactivity seemed to be stronger in lymphomas of T cell system. (3) MT-1 and MB-1 immunoreactivity did not correlate perfectly with LCA positivity. And (4) MT-1 ant MB-1 provide useful tools to dictate markers for T and B cell lymphomas in routinely fixed and paraffin-embedded tissue

Respirovirus C protein inhibits activation of type I interferon receptor-associated kinases to block JAK-STAT signaling.

Respirovirus C protein blocks the type I interferon (IFN)-stimulated activation of the JAK-STAT pathway. It has been reported that C protein inhibits IFN-α-stimulated tyrosine phosphorylation of STATs, but the underlying mechanism is poorly understood. Here, we show that the C protein of Sendai virus (SeV), a member of the Respirovirus genus, binds to the IFN receptor subunit IFN-α/β receptor subunit (IFNAR)2 and inhibits IFN-α-stimulated tyrosine phosphorylation of the upstream receptor-associated kinases, JAK1 and TYK2. Analysis of various SeV C mutant (Cm) proteins demonstrates the importance of the inhibitory effect on receptor-associated kinase phosphorylation for blockade of JAK-STAT signaling. Furthermore, this inhibitory effect and the IFNAR2 binding capacity are observed for all the respirovirus C proteins examined. Our results suggest that respirovirus C protein inhibits activation of the receptor-associated kinases JAK1 and TYK2 possibly through interaction with IFNAR2