32 research outputs found

    Vegetable oils, promising feedstock of the hydro-refining process

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    A pilot study of cell-mediated gene therapy for spinal cord injury in mini pigs

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    © 2017 Elsevier B.V.Currently, in clinical practice there is no efficient way to overcome the sequences of neurodegeneration after spinal cord traumatic injury. Using a new experimental model of spinal cord contusion injury on miniature pigs, we proposed to deliver therapeutic genes encoding vascular endothelial growth factor (VEGF), glial cell line-derived neurotrophic factor (GDNF) and neural cell adhesion molecule (NCAM) to the damaged area, using umbilical cord blood mononuclear cells (UCBC). In this study, genetically engineered UCBC (2 × 106 cells in 200 ml of saline) were injected intrathecally to mini-pigs 10 days after SCI. Control and experimental mini pigs were observed for 60 days after surgery. Histological, electrophysiological, and clinical evaluation demonstrated significant improvement in animal treated with genetically engineered UCBCs. Difference in recovery of the somatosensory evoked potentials and in histological findings in control and treated animals support the positive effect of the gene-cell constriction for recovery after spinal cord injury. Results of this study suggest that transplantation of UCBCs simultaneously transduced with three recombinant adenoviruses Ad5-VEGF, Ad5-GDNF and Ad5-NCAM represent a novel potentially successful approach for treatment of spinal cord injury

    Послеоперационная когнитивная дисфункция – является ли она проблемой для анестезиолога-реаниматолога?

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    Postoperative cognitive dysfunction is a cognitive impairment developing in the postoperative period and confirmed by the data of neuropsychological testing. The article presents the results of a survey among practicing anesthesiologists-resuscitators in the Russian Federation on the problem of postoperative cognitive dysfunction. Methods. From January 21, 2019 to April 26, 2019, the survey was conducted among practicing anesthesiology and resuscitation physicians from 20 different regions of the Russian Federation, both in the online format and on paper. 340 questionnaires were analyzed, including 11 questions about the problem of cognitive impairment in the postoperative period. Microsoft Excel, Survio, Statistica 8.0 were used for statistical processing of the results.Results. It was revealed that 74.7% of respondents were aware of the problem of postoperative cognitive impairment. At the same time, doctors ranked second in terms of the frequency of clinical manifestations, symptoms that were more characteristic of postoperative delirium. 18.5% had difficulty in describing the clinical manifestations of postoperative cognitive disorders. Analysis of the answers on the diagnosis of postoperative cognitive dysfunction showed that 45.3% of specialists considered neuropsychological testing mandatory in all patients undergoing surgery, 40% noted the need for mandatory testing of the cognitive functions in patients at risk, but 37.6% of doctors were not able to name a single method, and 34% answered that doctors of other specialties were engaged in neuropsychological testing. The survey results also showed the absence of a single treatment strategy for cognitive dysfunction.Сonclusions. The analysis of respondents' answers revealed insufficient awareness of specialists about clinical manifestations, diagnosis and treatment of postoperative cognitive dysfunction. Послеоперационная когнитивная дисфункция представляет собой когнитивные нарушения, развивающиеся в послеоперационном периоде, подтвержденные данными нейропсихологического тестирования. В статье представлены результаты анкетирования практикующих врачей анестезиологов-реаниматологов Российской Федерации о проблеме послеоперационной когнитивной дисфункции.Методика. Проведено анонимное анкетирование практикующих врачей анестезиологов-реаниматологов 20 различных регионов Российской Федерации путем онлайн-опроса или с использованием бумажных носителей в период с 21 января по 26 апреля 2019 г. Проанализировано 340 анкет, включавших 11 вопросов о проблеме когнитивных нарушений в послеоперационном периоде. Статистическую обработку результатов выполняли с использованием программ Microsoft Excel, Survio, Statistica 8.0.Результаты. Выявлено, что 74,7% респондентов осведомлены о проблеме послеоперационных нарушений когнитивной сферы. При этом на втором месте по частоте клинических проявлений врачи называли симптомы, в большей степени характерные для послеоперационного делирия. У 18,5% возникли затруднения в описании клинических проявлений послеоперационных когнитивных расстройств. Анализ ответов, посвященных диагностике, показал, что 45,3% специалистов считают проведение нейропсихологического тестирования обязательным у всех пациентов, которым планируются оперативные вмешательства, 40% отметили необходимость обязательного обследования когнитивной сферы у пациентов группы риска, однако 37,6% врачей не смогли назвать ни одного метода, а 34% ответили, что нейропсихологическим тестированием занимаются врачи других специальностей. Результаты опроса также показали отсутствие единой лечебной стратегии в отношении когнитивных расстройств.Выводы. Анализ ответов респондентов выявил недостаточную информированность специалистов в области клинических проявлений, диагностики и лечения послеоперационной когнитивной дисфункции

    Спорные термины в анестезиологии и реаниматологии

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    Medical vocabulary is a part of the Russian language used by the professional community. Most of the terms are borrowed from other languages and poorly understood by most native speakers. The development of medicine, in particular, anesthesiology and resuscitation, leads to the emergence of many new conditions, symptoms, syndromes and their defnitions. The latter often cause controversy among doctors. The article is devoted to some aspects of the appearance of controversial terms in anesthesiology and resuscitation to discuss them.Медицинская лексика – часть русского языка, используемая профессиональным сообществом. Большинство отечественных медицинских терминов заимствованы из других языков и малопонятны большинству носителей языка. Развитие медицины, в частности анестезиологии и реаниматологии, приводит к появлению множества новых симптомов, синдромов, методов интенсивной терапии и, соответственно, их номинации, оформление и содержание которой нередко вызывают недопонимание и споры среди врачей. Статья посвящена обсуждению отдельных аспектов трактовки терминов в анестезиологии и реаниматологии, вызывающих наиболее частые разногласия в последнее время

    Postoperative cognitive dysfunction – is it a problem for the anesthesiologist-intensivist?

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    Postoperative cognitive dysfunction is a cognitive impairment developing in the postoperative period and confirmed by the data of neuropsychological testing. The article presents the results of a survey among practicing anesthesiologists-resuscitators in the Russian Federation on the problem of postoperative cognitive dysfunction. Methods. From January 21, 2019 to April 26, 2019, the survey was conducted among practicing anesthesiology and resuscitation physicians from 20 different regions of the Russian Federation, both in the online format and on paper. 340 questionnaires were analyzed, including 11 questions about the problem of cognitive impairment in the postoperative period. Microsoft Excel, Survio, Statistica 8.0 were used for statistical processing of the results.Results. It was revealed that 74.7% of respondents were aware of the problem of postoperative cognitive impairment. At the same time, doctors ranked second in terms of the frequency of clinical manifestations, symptoms that were more characteristic of postoperative delirium. 18.5% had difficulty in describing the clinical manifestations of postoperative cognitive disorders. Analysis of the answers on the diagnosis of postoperative cognitive dysfunction showed that 45.3% of specialists considered neuropsychological testing mandatory in all patients undergoing surgery, 40% noted the need for mandatory testing of the cognitive functions in patients at risk, but 37.6% of doctors were not able to name a single method, and 34% answered that doctors of other specialties were engaged in neuropsychological testing. The survey results also showed the absence of a single treatment strategy for cognitive dysfunction.Сonclusions. The analysis of respondents' answers revealed insufficient awareness of specialists about clinical manifestations, diagnosis and treatment of postoperative cognitive dysfunction

    A pilot study of cell-mediated gene therapy for spinal cord injury in mini pigs

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    © 2017 Elsevier B.V.Currently, in clinical practice there is no efficient way to overcome the sequences of neurodegeneration after spinal cord traumatic injury. Using a new experimental model of spinal cord contusion injury on miniature pigs, we proposed to deliver therapeutic genes encoding vascular endothelial growth factor (VEGF), glial cell line-derived neurotrophic factor (GDNF) and neural cell adhesion molecule (NCAM) to the damaged area, using umbilical cord blood mononuclear cells (UCBC). In this study, genetically engineered UCBC (2 × 106 cells in 200 ml of saline) were injected intrathecally to mini-pigs 10 days after SCI. Control and experimental mini pigs were observed for 60 days after surgery. Histological, electrophysiological, and clinical evaluation demonstrated significant improvement in animal treated with genetically engineered UCBCs. Difference in recovery of the somatosensory evoked potentials and in histological findings in control and treated animals support the positive effect of the gene-cell constriction for recovery after spinal cord injury. Results of this study suggest that transplantation of UCBCs simultaneously transduced with three recombinant adenoviruses Ad5-VEGF, Ad5-GDNF and Ad5-NCAM represent a novel potentially successful approach for treatment of spinal cord injury

    A pilot study of cell-mediated gene therapy for spinal cord injury in mini pigs

    Get PDF
    © 2017 Elsevier B.V.Currently, in clinical practice there is no efficient way to overcome the sequences of neurodegeneration after spinal cord traumatic injury. Using a new experimental model of spinal cord contusion injury on miniature pigs, we proposed to deliver therapeutic genes encoding vascular endothelial growth factor (VEGF), glial cell line-derived neurotrophic factor (GDNF) and neural cell adhesion molecule (NCAM) to the damaged area, using umbilical cord blood mononuclear cells (UCBC). In this study, genetically engineered UCBC (2 × 106 cells in 200 ml of saline) were injected intrathecally to mini-pigs 10 days after SCI. Control and experimental mini pigs were observed for 60 days after surgery. Histological, electrophysiological, and clinical evaluation demonstrated significant improvement in animal treated with genetically engineered UCBCs. Difference in recovery of the somatosensory evoked potentials and in histological findings in control and treated animals support the positive effect of the gene-cell constriction for recovery after spinal cord injury. Results of this study suggest that transplantation of UCBCs simultaneously transduced with three recombinant adenoviruses Ad5-VEGF, Ad5-GDNF and Ad5-NCAM represent a novel potentially successful approach for treatment of spinal cord injury

    A pilot study of cell-mediated gene therapy for spinal cord injury in mini pigs

    No full text
    © 2017 Elsevier B.V.Currently, in clinical practice there is no efficient way to overcome the sequences of neurodegeneration after spinal cord traumatic injury. Using a new experimental model of spinal cord contusion injury on miniature pigs, we proposed to deliver therapeutic genes encoding vascular endothelial growth factor (VEGF), glial cell line-derived neurotrophic factor (GDNF) and neural cell adhesion molecule (NCAM) to the damaged area, using umbilical cord blood mononuclear cells (UCBC). In this study, genetically engineered UCBC (2 × 106 cells in 200 ml of saline) were injected intrathecally to mini-pigs 10 days after SCI. Control and experimental mini pigs were observed for 60 days after surgery. Histological, electrophysiological, and clinical evaluation demonstrated significant improvement in animal treated with genetically engineered UCBCs. Difference in recovery of the somatosensory evoked potentials and in histological findings in control and treated animals support the positive effect of the gene-cell constriction for recovery after spinal cord injury. Results of this study suggest that transplantation of UCBCs simultaneously transduced with three recombinant adenoviruses Ad5-VEGF, Ad5-GDNF and Ad5-NCAM represent a novel potentially successful approach for treatment of spinal cord injury

    Microalbuminuria in risk stratification of non-diabetic hypertesive patients

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    Microalbuminuria (MAU) and a reduction in glomerular filtration rate are independent predictors of cardiovascular morbidity and mortality. Aim was to assess the role of MAU in risk stratification of non-diabetic hypertensive patients. The subgroup of patients with MAU was much more likely to show the other signs of subclinical organ damage as compared to patients with left ventricular hypertrophy, carotid abnormalities or increased pulse wave velocity. Thus, MAU is the integral marker of subclinical organ damage
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