Diffraction-limited 3 μm spectroscopy of IRAS 04296+3429 and IRAS 05341+0852: Spatial extent of hydrocarbon dust emission and dust evolutionary sequence

We present 3 μm spectroscopy of the carbon-rich protoplanetary nebulae IRAS 04296+3429 and IRAS 05341+0852, conducted with the adaptive optics system at the Subaru Telescope. We utilize the nearly diffraction-limited spectroscopy to probe the spatial extent of the hydrocarbon dust emitting zone. We find a hydrocarbon emission core extending up to 100-160 mas from the center of IRAS 04296+3429, corresponding to a physical diameter of 400-640 AU, assuming a distance of 4 kpc. However, we find that IRAS 05341+0852 is not spatially resolved with this instrumentation. The physical extent of these protoplanetary nebulae, along with the reanalyzed data of IRAS 22272+5435 published previously, suggests a correlation between the physical extent of the hydrocarbon dust emission and the spectral evolution of the aliphatic to aromatic features in these post-AGB stars. These measurements represent the first direct test of the proposed chemical synthesis route of carbonaceous dust in the circumstellar environment of evolved stars. © 2007, The American Astronomical Society, Ail rights reserved.published_or_final_versio

Renal tubular Sirt1 attenuates diabetic albuminuria by epigenetically suppressing Claudin-1 overexpression in podocytes

Sirtuin 1 (Sirt1), a NAD[superscript +]-regulated deacetylase with numerous known positive effects on cellular and whole-body metabolism, is expressed in the renal cortex and medulla. It is known to have protective effects against age-related disease, including diabetes. Here we investigated the protective role of Sirt1 in diabetic renal damage. We found that Sirt1 in proximal tubules (PTs) was downregulated before albuminuria occurred in streptozotocin-induced or obese (db/db) diabetic mice. PT-specific SIRT1 transgenic and Sirt1 knockout mice showed prevention and aggravation of the glomerular changes that occur in diabetes, respectively, and nondiabetic knockout mice exhibited albuminuria, suggesting that Sirt1 in PTs affects glomerular function. Downregulation of Sirt1 and upregulation of the tight junction protein Claudin-1 by SIRT1-mediated epigenetic regulation in podocytes contributed to albuminuria. We did not observe these phenomena in 5/6 nephrectomized mice. We also demonstrated retrograde interplay from PTs to glomeruli using nicotinamide mononucleotide (NMN) from conditioned medium, measurement of the autofluorescence of photoactivatable NMN and injection of fluorescence-labeled NMN. In human subjects with diabetes, the levels of SIRT1 and Claudin-1 were correlated with proteinuria levels. These results suggest that Sirt1 in PTs protects against albuminuria in diabetes by maintaining NMN concentrations around glomeruli, thus influencing podocyte function.Japan. Ministry of Education, Culture, Sports, Science and Technology (Grant 22790800