A micro cell lysis device

A new micromachined cell lysis device is developed. It is designed for miniature bio-analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. It consists of muti-electrode pairs to apply electric fields to cells. We adopt the means of using electric field lysing because it can greatly simplify purification steps for preparation of biological samples, when compared to conventional chemical methods. Yeast, Chinese cabbage, radish cells and E. coli are tested with the device. The lysis of yeast, Chinese cabbage, radish cells is observed by a microscope. The experimental observation suggests E. coli are also lysed by the pulsed electric field. The range of electric field for the lysis is on the order of 1 kV/cm to 10 kV/cm. In addition, for practical reasons, we reduce the voltage required for lysing to less than 10 V by making the electrode gap on the order of microns

Flexible parylene actuator for micro adaptive flow control

This paper describes the first flexible parylene electrostatic actuator valves intended for micro adaptive flow control for the future use on the wings of micro-air-vehicle (MAV). The actuator diaphragm is made of two layers of parylene membranes with offset vent holes. Without electrostatic actuation, air can move freely from one side of the skin to the other side through the vent holes. With actuation, these vent holes are sealed and the airflow is controlled. The membrane behaves as a complete diaphragm. We have successfully demonstrated this function using a 2-mm x 2-mm parylene diaphragm electrostatic actuator valves. This work also includes the novel anti-stiction technology that is crucial to make such large-area parylene actuator diaphragm with the combined use of anti-stiction posts, self-assembled monolayers (SAM), surface roughening, and bromine trifluoride (BrFe) dry etching. With the help of SAM treatment, the operating voltage is lowered from 30 volts to 13 volts. The load deflection method is then used to measure the effective thickness of the composite diaphragm. The flexible parylene diaphragm can be deflected up to 100 μm when 150 Torr of pressure is applied. The result is fitted into a theoretical model and yields an effective thickness of 5.9 μm, which is agreeable with the actual thickness of 5.6 μm, thus proves the functionality of the device

Optimal Capacitive Load Matching of Micro Electret Power Generators

This paper presents a model of micro-electret power generators. This model uses two capacitors with fixed charge density and variable area to model the actual micro electret power generator. Simulations of power output with capacitive loads and resistive loads are presented. The power output decreases as the load capacitance increases while it increases as the resistive load increases. To verify the model and simulation results, power output generation experiments are performed and the results confirm the simulation. To collect useful energy from the power generator, a small capacitive load, rather than a resistive load, is required

Disposable Integrated Microfluidic Biochip for Blood Typing by Plastic Microinjection Moulding

Blood typing is the most important test for both transfusion recipients and blood donors. In this paper, a low cost disposable blood typing integrated microfluidic biochip has been designed, fabricated and characterized. In the biochip, flow splitting microchannels, chaotic micromixers, reaction microchambers and detection microfilters are fully integrated. The loaded sample blood can be divided by 2 or 4 equal volumes through the flow splitting microchannel so that one can perform 2 or 4 blood agglutination tests in parallel. For the purpose of obtaining efficient reaction of agglutinogens on red blood cells (RBCs) and agglutinins in serum, we incorporated a serpentine laminating micromixer into the biochip, which combines two chaotic mixing mechanisms of splitting/recombination and chaotic advection. Relatively large area reaction microchambers were also introduced for the sake of keeping the mixture of the sample blood and serum during the reaction time before filtering. The gradually decreasing multi-step detection microfilters were designed in order to effectively filter the reacted agglutinated RBCs, which show the corresponding blood group. To achieve the cost-effectiveness of the microfluidic biochip for disposability, the biochip was realized by the microinjection moulding of COC (cyclic olefin copolymer) and thermal bonding of two injection moulded COC substrates in mass production with a total fabrication time of less than 20 min. Mould inserts of the biochip for the microinjection moulding were fabricated by SU-8 photolithography and the subsequent nickel electroplating process. Human blood groups of A, B and AB have been successfully determined with the naked eye, with 3 mu l of the whole sample bloods, by means of the fabricated biochip within 3 min.X11100104sciescopu

Near tip strain evolution under cyclic loading

The concept of ratchetting strain as a crack driving force in controlling crack growth has previouslybeen explored at Portsmouth using numerical approaches for nickel-based superalloys. In this paper, we reportthe first experimental observations of the near-tip strain evolution as captured by the Digital Image Correlation(DIC) technique on a compact tension specimen of stainless steel 316L. The evolution of the near-tip strainswith loading cycles was studied whilst the crack tip was maintained stationary. The strains were monitored overthe selected distances from the crack tip for a given number of cycles under an incremental loading regime. Theresults show that strain ratchetting does occur with load cycling, and is particularly evident close to the crack tipand under higher loads. A finite element model has been developed to simulate the experiments and thesimulation results are compared with the DIC measurements

Water Soluble Hyperbranched Polymers from Controlled Radical Homopolymerization of PEG Diacrylate

Hyperbranched PEG-based polymers were synthesized through homopolymerization of PEG diacrylate via enhanced intermolecular branching and showed a concentration-dependent thermoresponsive property.</p

Health status of older adults with Type 2 diabetes mellitus after aerobic or resistance training: A randomised trial

10.1186/1477-7525-9-59Health and Quality of Life Outcomes9

Robot Control by ERPs of Brain Waves

This paper presented the technique of robot control by event-related potentials (ERPs) of brain waves. Based on the proposed technique, severe physical disabilities can free browse outside world. A specific component of ERPs, N2P3, was found and used to control the movement of robot and the view of camera on the designed brain-computer interface (BCI). Users only required watching the stimuli of attended button on the BCI, the evoked potentials of brain waves of the target button, N2P3, had the greatest amplitude among all control buttons. An experimental scene had been constructed that the robot required walking to a specific position and move the view of camera to see the instruction of the mission, and then completed the task. Twelve volunteers participated in this experiment, and experimental results showed that the correct rate of BCI control achieved 80% and the average of execution time was 353 seconds for completing the mission. Four main contributions included in this research: (1) find an efficient component of ERPs, N2P3, for BCI control, (2) embed robot's viewpoint image into user interface for robot control, (3) design an experimental scene and conduct the experiment, and (4) evaluate the performance of the proposed system for assessing the practicability