53 research outputs found
ASPEK SANITASI DAN HIGIENE DI KANTIN ASRAMA TINGKAT PERSIAPAN BERSAMA (TPB) INSTITUT PERTANIAN BOGOR
The major purpose of this research was to analyze sanitation and hygiene at TPB - IPB Dormitoryâs Canteens. Microbiological analysis (sanitation analysis) was done on chefâs hands and some utensils (eating plate, serving plate, and chopping board). There were a lot of Staphylococcusfound in food handlerâs hands (19-57 CFU). This research showed that there was no tendency which was the worst canteen from the three canteens at boyâs dormitory or girlâs dormitory. The statistical analysis showed that the location of the canteen and kind of utensils influenced the total Enterobacter. The chefâs hands of C1 and A3 canteens contained E. coli bacteria, but the other chefâs hands, did not. Eating plate, serving plate, and chopping board from Canteens A3 and C1 were not safe due to the number of microbes they had (1.08x102-3.6x102 CFU/cm2). All utensils at canteen A1 were safe. C2âs and C3âs chopping boards were not safe, but their eating plate and serving plate were safe
Reactive oxygen species and male reproductive hormones
Reports of the increasing incidence of male infertility paired with decreasing semen quality have triggered studies
on the effects of lifestyle and environmental factors on the male reproductive potential. There are numerous exogenous
and endogenous factors that are able to induce excessive production of reactive oxygen species (ROS) beyond that of
cellular antioxidant capacity, thus causing oxidative stress. In turn, oxidative stress negatively affects male reproductive
functions and may induce infertility either directly or indirectly by affecting the hypothalamus-pituitary-gonadal (HPG)
axis and/or disrupting its crosstalk with other hormonal axes. This review discusses the important exogenous and
endogenous factors leading to the generation of ROS in different parts of the male reproductive tract. It also highlights
the negative impact of oxidative stress on the regulation and cross-talk between the reproductive hormones. It further
describes the mechanism of ROS-induced derangement of male reproductive hormonal profiles that could ultimately
lead to male infertility. An understanding of the disruptive effects of ROS on male reproductive hormones would
encourage further investigations directed towards the prevention of ROS-mediated hormonal imbalances, which in turn
could help in the management of male infertility
ASPEK SANITASI DAN HIGIENE DI KANTIN ASRAMA TINGKAT PERSIAPAN BERSAMA (TPB) INSTITUT PERTANIAN BOGOR
800x600 Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 The major purpose of this research was to analyze sanitation and hygiene at TPB - IPB Dormitoryâs Canteens. Microbiological analysis (sanitation analysis) was done on chefâs hands and some utensils (eating plate, serving plate, and chopping board). There were a lot of Staphylococcus found in food handlerâs hands (19-57 CFU). This research showed that there was no tendency which was the worst canteen from the three canteens at boyâs dormitory or girlâs dormitory. The statistical analysis showed that the location of the canteen and kind of utensils influenced the total Enterobacter. The chefâs hands of C1 and A3 canteens contained E. coli bacteria, but the other chefâs hands, did not. Eating plate, serving plate, and chopping board from Canteens A3 and C1 were not safe due to the number of microbes they had (1.08x102-3.6x102 CFU/cm2). All utensils at canteen A1 were safe. C2âs and C3âs chopping boards were not safe, but their eating plate and serving plate were safe. Keywords: sanitation, hygiene, Dormitoryâs Canteen, microbiology test, utensil</em
Sulfinylated Azadecalins act as functional mimics of a pollen germination stimulant in Arabidopsis pistils
Polarized cell elongation is triggered by small molecule cues during development of diverse organisms. During plant reproduction, pollen interactions with the stigma result in the polar outgrowth of a pollen tube, which delivers sperm cells to the female gametophyte to effect double fertilization. In many plants, pistils stimulate pollen germination. However, in Arabidopsis, the effect of pistils on pollen germination and the pistil factors that stimulate pollen germination remain poorly characterized. Here, we demonstrate that stigma, style, and ovules in Arabidopsis pistils stimulate pollen germination. We isolated an Arabidopsis pistil extract fraction that stimulates Arabidopsis pollen germination, and employed ultrahigh resolution ESI FT-ICR and MS/MS techniques to accurately determine the mass (202.126 daltons) of a compound that is specifically present in this pistil extract fraction. Using the molecular formula (C
10
H
19
NOS) and tandem mass spectral fragmentation patterns of the
m/z
(mass to charge ratio) 202.126 ion, we postulated chemical structures, devised protocols, synthesized
N
-Methanesulfinyl 1- and 2-azadecalins that are close structural mimics of the
m/z
202.126 ion, and showed that they are sufficient to stimulate Arabidopsis pollen germination
in vitro
(30 ”M stimulated ~50% germination) and elicit accession-specific response. Although
N
-Methanesulfinyl 2-azadecalin stimulated pollen germination in three species of Lineage I of Brassicaceae, it did not induce a germination response in
Sisymbrium irio
(Lineage II of Brassicaceae) and tobacco, indicating that activity of the compound is not random. Our results show that Arabidopsis pistils promote germination by producing azadecalin-like molecules to ensure rapid fertilization by the appropriate pollen
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