19 research outputs found
Synthesis of nucleoside 5′-O-α,β-methylene-β-triphosphates and evaluation of their potency towards inhibition of HIV-1 reverse transcriptase
Solid-Phase Synthesis of Symmetrical 5,5-Dinucleoside Mono-, Di-, Tri-, and Tetraphosphodiesters
Preparation of Synthetically Challenging Nucleotides Using Cyanoethyl P-Imidazolides and Microwaves
Protein pyrophosphorylation by inositol pyrophosphates is a posttranslational event
In a previous study, we showed that the inositol pyrophosphate diphosphoinositol pentakisphosphate (IP7) physiologically phosphorylates mammalian and yeast proteins. We now report that this phosphate transfer reflects pyrophosphorylation. Thus, proteins must be prephosphorylated by ATP to prime them for IP7 phosphorylation. IP7 phosphorylates synthetic phosphopeptides but not if their phosphates have been masked by methylation or pyrophosphorylation. Moreover, IP7 phosphorylated peptides are more acid-labile and more resistant to phosphatases than ATP phosphorylated peptides, indicating a different type of phosphate bond. Pyrophosphorylation may represent a novel mode of signaling to proteins