1,854 research outputs found
Associations of perceived interparental relationship, family harmony and family happiness with smoking intention in never-smoking Chinese children and adolescents: a cross-sectional study
published_or_final_versio
Does electronic cigarette use predict abstinence from conventional cigarettes among smokers in Hong Kong?
published_or_final_versio
Knowledge on harms of thirdhand smoking is associated with greater support on smokefree policies
The 17th World Conference on Tobacco or Health (WCTOH), Cape Town, South Africa, 7-9 March 201
Wigner Crystallization in a Quasi-3D Electronic System
When a strong magnetic field is applied perpendicularly (along z) to a sheet
confining electrons to two dimensions (x-y), highly correlated states emerge as
a result of the interplay between electron-electron interactions, confinement
and disorder. These so-called fractional quantum Hall (FQH) liquids form a
series of states which ultimately give way to a periodic electron solid that
crystallizes at high magnetic fields. This quantum phase of electrons has been
identified previously as a disorder-pinned two-dimensional Wigner crystal with
broken translational symmetry in the x-y plane. Here, we report our discovery
of a new insulating quantum phase of electrons when a very high magnetic field,
up to 45T, is applied in a geometry parallel (y-direction) to the
two-dimensional electron sheet. Our data point towards this new quantum phase
being an electron solid in a "quasi-3D" configuration induced by orbital
coupling with the parallel field
Intraspecfic variation in cold-temperature metabolic phenotypes of Arabidopsis lyrata ssp petraea
Atmospheric temperature is a key factor in determining the distribution of a plant species. Alongside this, plant populations growing at the margin of their range may exhibit traits that indicate genetic differentiation and adaptation to their local abiotic environment. We investigated whether geographically separated marginal populations of Arabidopsis lyrata ssp. petraea have distinct metabolic phenotypes associated with exposure to cold temperatures. Seeds of A. petraea were obtained from populations along a latitudinal gradient, namely Wales, Sweden and Iceland and grown in a controlled cabinet environment. Mannose, glucose, fructose, sucrose and raffinose concentrations were different between cold treatments and populations, especially in the Welsh population, but polyhydric alcohol concentrations were not. The free amino acid compositions were population specific, with fold differences in most amino acids, especially in the Icelandic populations, with gross changes in amino acids, particularly those associated with glutamine metabolism. Metabolic fingerprints and profiles were obtained. Principal component analysis (PCA) of metabolite fingerprints revealed metabolic characteristic phenotypes for each population and temperature. It is suggested that amino acids and carbohydrates were responsible for discriminating populations within the PCA. Metabolite fingerprinting and profiling has proved to be sufficiently sensitive to identify metabolic differences between plant populations at different atmospheric temperatures. These findings show that there is significant natural variation in cold metabolism among populations of A. l. petraea which may signify plant adaptation to local climates
Inverted Expression Profiles of Sex-Biased Genes in Response to Toxicant Perturbations and Diseases
10.1371/journal.pone.0056668PLoS ONE82
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.
p53 and its major E3 ligase Mdm2 are both ubiquitinated and targeted to the proteasome for degradation. Despite the importance of this in regulating the p53 pathway, little is known about the mechanisms of proteasomal recognition of ubiquitinated p53 and Mdm2. In this study, we show that knockdown of the proteasomal ubiquitin receptor S5a/PSMD4/Rpn10 inhibits p53 protein degradation and results in the accumulation of ubiquitinated p53. Overexpression of a dominant-negative deletion of S5a lacking its ubiquitin-interacting motifs (UIM)s, but which can be incorporated into the proteasome, also causes the stabilization of p53. Furthermore, small-interferring RNA (siRNA) rescue experiments confirm that the UIMs of S5a are required for the maintenance of low p53 levels. These observations indicate that S5a participates in the recognition of ubiquitinated p53 by the proteasome. In contrast, targeting S5a has no effect on the rate of degradation of Mdm2, indicating that proteasomal recognition of Mdm2 can be mediated by an S5a-independent pathway. S5a knockdown results in an increase in the transcriptional activity of p53. The selective stabilization of p53 and not Mdm2 provides a mechanism for p53 activation. Depletion of S5a causes a p53-dependent decrease in cell proliferation, demonstrating that p53 can have a dominant role in the response to targeting S5a. This study provides evidence for alternative pathways of proteasomal recognition of p53 and Mdm2. Differences in recognition by the proteasome could provide a means to modulate the relative stability of p53 and Mdm2 in response to cellular signals. In addition, they could be exploited for p53-activating therapies. This work shows that the degradation of proteins by the proteasome can be selectively dependent on S5a in human cells, and that this selectivity can extend to an E3 ubiquitin ligase and its substrate
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