Keanekaragaman Spesies Lamun di Kabupaten Bangka Selatan Provinsi Kepulauan Bangka Belitung

Marine area which is planted by seagrass called as seagrass beds. Seagrass is one of the typical ecosystems in marine waters. Bangka Belitung Province is one of the areas in Indonesia that has seagrass ecosystem. This study aims to determine the diversity, density, and coverage area of seagrass beds in the waters of South Bangka Regency. The research was conducted in Tukak, Tanjung Kerasak, and Tanjung Kemirai. The data is collected using quadratic transect method and also data of physics-chemical parameters such as temperature, salinity, pH, depth, velocity and water substrate. The results of the research showed that 7 species of seagrasses in South Bangka Regency are Cymodocea serrulata, Cymodocea rotundata, Halodule uninervis, Enhalus acoroides, Thalassia hemprichii, Syringodium isoetifolium and Halophila minor. Seagrass in Lempeng waters which has the highest density is Cymodocea serrulata, about 58 ind/m2. The highest frequency of seagrass species in Tukak is Thalassia hemprichii about 1%. Seagrass species of Cymodocea serrulata has the highest cover percentage in Lempeng waters about 29.56%

Efek Konsentrasi Sublethal Fenol Terhadap Total Haemocyte Count (THC) Dan Histologi Insang Kepiting Bakau (Scylla Serata)

Hadirnya fenol yang melebihi batas ambang ke dalam ekosistem perairan dapat menjadi stresor kimia bagi organisme akuatik, termasuk juga bagi ekosistem muara oleh karena muara (estuaria) merupakan daerah pertemuan antara air tawar dari perairan sungai dan air laut sehingga berpotensi mengandung bahan kimia antropogenik. Kepiting bakau (Scylla spp.) memiliki siklus hidup yang sebagian besar berada pada ekosistem mangrove dan umum digunakan dalam studi ekotoksisitas. Total Haemocyte Count (THC) dan histologi organ insang dapat menginformasikan Perubahan histologi akibat stressor oleh karena paparan toksik, terutama fenol. Perlakuan sublethal fenol dengan konsentrasi yang berbeda terhadap kepiting bakau (Scylla serata) pada hari ke-1, hari ke-3, hari ke-5 dan hari ke-8 tidak terdapat perbedaan yang signifikan (p> 0,05) (Lampiran) terhadap rata-rata Total Haemocyte Count (THC). Namun pada hari ke-7 menunjukkan penurunan jumlah THC pada tiap perlakuan bila dibandingkan dengan kontrol (tanpa perlakuan). Perubahan histologi yang terjadi meningkat sebanding dengan konsentrasi fenol yang diperlakukan terhadap kepiting bakau (S. serata). Perubahan histologi yang terjadi adalah kerusakan struktur dari lamela insang (l) dan bagian terluar sinus lamela atau outer lamellar sinuses (ols) yang meliputi infiltrasi hemosit, hiperplasia maupun nekrosis

Purification and Characterization of Aeromonas Media Klu 11.16 Chitosanase Isolated From Shrimp Waste

Our previous study found that KLU 11.16, isolated from shrimp waste secreted chitinolytic enzymes. The crude enzyme was interesting since their chitooligosccharide was able to inhibit some pathogenic bacteria. In this study we report a purification and characterization of the chitosanase enzyme produced and the identification of the KLU 11.16. Purification of the enzyme was done two steps by ion exchange chromatography followed by gel filtration. Two out of 4 peaks from Gel Filtration step, i.e. fraction 16 and 33 were capable of hydrolyzing 100% deacetylated chitosan, indicating that both fractions contained chitosanase enzyme. The enzyme from fraction 16 had approximate molecular weight of 98.3 kDa. The enzyme worked optimally at temperature of 300C, and pH 6. Addition of Ca2+, Fe2+, K+, Na+ ions in the form of Cl2 salt and detergent Triton X-100 increased the enzyme activity, while Co2+, Mn2+ and Zn2+ ions in the same concentration decreased the enzyme acitivity. Addition of EDTA and SDS significantly decreased the enzyme activity. Molecular based identification revealed that KLU 11.16 was 99% similar to Aeromonas media

The expression of the hormone estrogen in the gonads of green Asian green mussel (Perna viridis) as biomarker of estrogenic compound on the Banyu Urip s and Kenjeran Waters, East Java

Asian Green Mussel is one the ideal bivalves to investigate the estrogenic contamination because of its life that is sedentary, attached, and sensitive to environmental changes. Estrogenic compound is very harmful to organisms because it can resemble the estrogen hormone (xenoestrogen) and it has high accumulation power in the body. Xenoestrogen can bind to ER expressed in the E2 form. The research method used in this research was ELISA kit. Based on the research result, the ER in the body of Asian Green Mussel in Banyu Urip is 0.125 ng/l in male and 8.2 ng/l in the female. In Kenjeran waters, Perna Viridis is 5.8 ng/l in male and 2.9 ng/l in the female, the estradiol concentrations in water and body of male and female of Asian Green Mussel in Banyu Urip are 4 ng/l; 0.25 ng/l and 30.6 ng/l, respectively. Meanwhile, in Kenjeran are 80 ng/l; 39.9 ng/l and 5.9 ng/l, respectively. E2 Gonads of male Asian Green Mussel in Banyu Urip tend to be lower than in Kenjeran. This is due to the presence of estrogenic compounds that will disrupt the hormone system within the Asian Green Mussel’s body. Thus the Asian Green Mussel synthesizes the estrogen hormone more than the usual level. If this condition continues, it can change the Asian Green Mussel that is supposed to be male changes in female due to excessive synthesis of estrogen hormone. This change may cause an unbalanced condition that will affect the population within an aquatic ecosystem