1,025 research outputs found
PP-039 High use rates of tobacco among adolescents in rural areas of the Indian state of Uttarakhand: The role of fathers
First aid knowledge among health assigned teachers of primary schools
Background: Every parents of a child knows that it is hard enough to keep their child safe at home but when child goes off to school there is a whole new set up of challenges. Parents totally entrust their children to the schools. They rely on the teachers as their second parents in the institute for learning. They know for a fact that their educators will want no harm to come their way. While they are busy working, they fully believe that their children are well cared for. On the other hand, the teachers have the responsibility to keep an eye on their students. They should make sure that they are comfortable and secured within the four walls of their school. This then calls for their ability to handle even the most stressful situations. So much more, the school staff, especially the health teacher has to be equipped with the knowledge of first aid.Methods: Non-experimental survey study was conducted to assess the knowledge of first-aid among Health assigned teacher working in selected schools of Dehradun city. Using purposive sampling technique 40 samples of different schools of Dehradun city was adopted to select the area.Results: Result shows that the majority of health assign teachers were having average knowledge about first aid i.e. 29 (72.5%) and 10 (25%) were having good knowledge and 1 (2.5%) was having poor knowledge regarding first aid. Majority of participant were female 21 (52.5%). The educational status of most participants was post graduate (67.5%). Majority of the schools were private 25 (62.5%). There is no significant association between socio-demographic variables i.e. Gender, level of schools, year of experience as a health assigned teacher and education provided by teacher, but qualifications of teachers and type of schools was found to have a significant association.(p> 0.05).Conclusions: First aid is the assistance given to any person suffering a sudden illness or injury, with care it is provided to preserve life and to prevent the condition from worsening, and for promoting recovery. It is important that health assigned teachers are equipped with strong, professional competencies. In order for health promotion actions to be sustainable in schools, teachers must be capable, competent and skilled health educators. As they are at prime position to contribute to a nations health gain through the provision of health education for future adults. Based on findings we can say that the health assigned teachers are having average knowledge regarding first aid. Therefore, the health assigned teachers must be equipped with appropriate and upgraded knowledge
SARS CoV-2 aggravates cellular metabolism mediated complications in COVID-19 infection.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the primary causative organism in corona virus disease-19 (COVID-19) infections, is a novel member of the human coronavirus family which was first identified in Wuhan, China, towards the end of 2019. This letter reveals new vital missing links in our current understanding of the mechanisms that lead to cell death triggered by ferroptotic stress in COVID-19 infection. It further reveal the importance of homocysteine mediated trans-sulfuration pathway in COVID-19 infection. Hence, Vitamin B6, folic acid, and Vitamin B12 should be incorporated in the treatment regimen for SARS CoV-2 infections to suppress complications, as the virus mediates altered host cell metabolism
Isolated radial head dislocation, a rare and easily missed injury in the presence of major distracting injuries: a case report
High velocity accidents can lead to major injuries – long bone fractures, abdominal trauma, pelvic fractures and chest injuries. These injuries can act as distracting factors during the initial assessment of a polytrauma patient and innocuous but significant smaller injuries can be missed. We present a rare case of isolated anterolateral radial head dislocation in a polytrauma patient
Small Quadrupole Deformation for the Dipole Bands in 112In
High spin states in In were investigated using Mo(O,
p3n) reaction at 80 MeV. The excited level have been observed up to 5.6 MeV
excitation energy and spin 20 with the level scheme showing three
dipole bands. The polarization and lifetime measurements were carried out for
the dipole bands. Tilted axis cranking model calculations were performed for
different quasi-particle configurations of this doubly odd nucleus. Comparison
of the calculations of the model with the B(M1) transition strengths of the
positive and negative parity bands firmly established their configurations.Comment: 10 pages, 11 figures, 2 table
Driving calmodulin protein towards conformational shift by changing ionization states of select residues
Proteins are complex systems made up of many conformational sub-states which are mainly determined by the folded structure. External factors such as solvent type, temperature, pH and ionic strength play a very important role in the conformations sampled by proteins. Here we study the conformational multiplicity of calmodulin (CaM) which is a protein that plays an important role in calcium signaling pathways in the eukaryotic cells. CaM can bind to a variety of other proteins or small organic compounds, and mediates different physiological processes by activating various enzymes. Binding of calcium ions and proteins or small organic molecules to CaM induces large conformational changes that are distinct to each interacting partner. In particular, we discuss the effect of pH variation on the conformations of CaM. By using the pKa values of the charged residues as a basis to assign protonation states, the conformational changes induced in CaM by reducing the pH are studied by molecular dynamics simulations. Our current view suggests that at high pH, barrier crossing to the compact form is prevented by repulsive electrostatic interactions between the two lobes. At reduced pH, not only is barrier crossing facilitated by protonation of residues, but also conformations which are on average more compact are attained. The latter are in accordance with the fluorescence resonance energy transfer experiment results of other workers. The key events leading to the conformational change from the open to the compact conformation are (i) formation of a salt bridge between the N-lobe and the linker, stabilizing their relative motions, (ii) bending of the C-lobe towards the N-lobe, leading to a lowering of the interaction energy between the two-lobes, (iii) formation of a hydrophobic patch between the two lobes, further stabilizing the bent conformation by reducing the entropic cost of the compact form, (iv) sharing of a Ca+2 ion between the two lobes
Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana
Mg-chelatase H subunit (CHLH) is a multifunctional protein involved in chlorophyll synthesis, plastid-to-nucleus retrograde signaling, and ABA perception. However, whether CHLH acts as an actual ABA receptor remains controversial. Here we present evidence that CHLH affects ABA signaling in stomatal guard cells but is not itself an ABA receptor. We screened ethyl methanesulfonate-treated Arabidopsis thaliana plants with a focus on stomatal aperture-dependent water loss in detached leaves and isolated a rapid transpiration in detached leaves 1 (rtl1) mutant that we identified as a novel missense mutant of CHLH. The rtl1 and CHLH RNAi plants showed phenotypes in which stomatal movements were insensitive to ABA, while the rtl1 phenotype showed normal sensitivity to ABA with respect to seed germination and root growth. ABA-binding analyses using 3H-labeled ABA revealed that recombinant CHLH did not bind ABA, but recombinant pyrabactin resistance 1, a reliable ABA receptor used as a control, showed specific binding. Moreover, we found that the rtl1 mutant showed ABA-induced stomatal closure when a high concentration of extracellular Ca2+ was present and that a knockout mutant of Mg-chelatase I subunit (chli1) showed the same ABA-insensitive phenotype as rtl1. These results suggest that the Mg-chelatase complex as a whole affects the ABA-signaling pathway for stomatal movements
Joint Evolutionary Trees: A Large-Scale Method To Predict Protein Interfaces Based on Sequence Sampling
The Joint Evolutionary Trees (JET) method detects protein interfaces, the core
residues involved in the folding process, and residues susceptible to
site-directed mutagenesis and relevant to molecular recognition. The approach,
based on the Evolutionary Trace (ET) method, introduces a novel way to treat
evolutionary information. Families of homologous sequences are analyzed through
a Gibbs-like sampling of distance trees to reduce effects of erroneous multiple
alignment and impacts of weakly homologous sequences on distance tree
construction. The sampling method makes sequence analysis more sensitive to
functional and structural importance of individual residues by avoiding effects
of the overrepresentation of highly homologous sequences and improves
computational efficiency. A carefully designed clustering method is parametrized
on the target structure to detect and extend patches on protein surfaces into
predicted interaction sites. Clustering takes into account residues'
physical-chemical properties as well as conservation. Large-scale application of
JET requires the system to be adjustable for different datasets and to guarantee
predictions even if the signal is low. Flexibility was achieved by a careful
treatment of the number of retrieved sequences, the amino acid distance between
sequences, and the selective thresholds for cluster identification. An iterative
version of JET (iJET) that guarantees finding the most likely interface residues
is proposed as the appropriate tool for large-scale predictions. Tests are
carried out on the Huang database of 62 heterodimer, homodimer, and transient
complexes and on 265 interfaces belonging to signal transduction proteins,
enzymes, inhibitors, antibodies, antigens, and others. A specific set of
proteins chosen for their special functional and structural properties
illustrate JET behavior on a large variety of interactions covering proteins,
ligands, DNA, and RNA. JET is compared at a large scale to ET and to Consurf,
Rate4Site, siteFiNDER|3D, and SCORECONS on specific structures. A significant
improvement in performance and computational efficiency is shown
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