TROP2 Expressed in the Trunk of the Ureteric Duct Regulates Branching Morphogenesis during Kidney Development

TROP2, a cell surface protein structurally related to EpCAM, is expressed in various carcinomas, though its function remains largely unknown. We examined the expression of TROP2 and EpCAM in fetal mouse tissues, and found distinct patterns in the ureteric bud of the fetal kidney, which forms a tree-like structure. The tip cells in the ureteric bud proliferate to form branches, whereas the trunk cells differentiate to form a polarized ductal structure. EpCAM was expressed throughout the ureteric bud, whereas TROP2 expression was strongest at the trunk but diminished towards the tips, indicating the distinct cell populations in the ureteric bud. The cells highly expressing TROP2 (TROP2high) were negative for Ki67, a proliferating cell marker, and TROP2 and collagen-I were co-localized to the basal membrane of the trunk cells. TROP2high cells isolated from the fetal kidney failed to attach and spread on collagen-coated plates. Using MDCK cells, a well-established model for studying the branching morphogenesis of the ureteric bud, TROP2 was shown to inhibit cell spreading and motility on collagen-coated plates, and also branching in collagen-gel cultures, which mimic the ureteric bud's microenvironment. These results together suggest that TROP2 modulates the interaction between the cells and matrix and regulates the formation of the ureteric duct by suppressing branching from the trunk during kidney development

Initial physics achievements of large helical device experiments

The Large Helical Device (LHD) experiments [O. Motojima, et al., Proceedings, 16th Conference on Fusion Energy, Montreal, 1996 (International Atomic Energy Agency, Vienna, 1997), Vol. 3, p. 437] have started this year after a successful eight-year construction and test period of the fully superconducting facility. LHD investigates a variety of physics issues on large scale heliotron plasmas (R = 3.9 m, a = 0.6 m), which stimulates efforts to explore currentless and disruption-free steady plasmas under an optimized configuration. A magnetic field mapping has demonstrated the nested and healthy structure of magnetic surfaces, which indicates the successful completion of the physical design and the effectiveness of engineering quality control during the fabrication. Heating by 3 MW of neutral beam injection (NBI) has produced plasmas with a fusion triple product of 8 X 10^18 keV m^3 s at a magnetic field of 1.5 T. An electron temperature of 1.5 keV and an ion temperature of 1.4 keV have been achieved. The maximum stored energy has reached 0.22 MJ, which corresponds to = 0.7%, with neither unexpected confinement deterioration nor visible magnetohydrodynamics (MHD) instabilities. Energy confinement times, reaching 0.17 s at the maximum, have shown a trend similar to the present scaling law derived from the existing medium sized helical devices, but enhanced by 50%. The knowledge on transport, MHD, divertor, and long pulse operation, etc., are now rapidly increasing, which implies the successful progress of physics experiments on helical currentless-toroidal plasmas

Thermal transport barrier in heliotron-type devices (Large Helical Device and Compact Helical System)

In the discharges of the Large Helical Device [O. Motojima et al., Proceedings of the 16th Conference on Fusion Energy, Montreal, 1996 (International Atomic Energy Agency, Vienna, 1997), Vol. 3, p. 437], a significant enhancement of the energy confinement has been achieved with an edge thermal transport barrier, which exhibits a sharp gradient at the edge. Key features associated with the barrier are quite different from those seen in tokamaks (i) almost no change in particle (including impurity) transport, (ii) a gradual formation of the barrier, (iii) a very high ratio of the edge temperature to the average temperature, (iv) no edge relaxation phenomenon. In the electron cyclotron heating (ECH) heated discharges in the Compact Helical System [K. Matsuoka et al., in Proceedings of the 12th International Conference on Plasma Physics and Controlled Nuclear Fusion Research, Nice, France, 1988 (International Atomic Energy Agency, Vienna, 1989), Vol. 2, p. 411], the internal electron transport barrier has been observed, which enhances the central electron temperature significantly. High shear of the radial electric field appears to suppress the turbulence in the core region and enhance the electron confinement there

Hypersensitivity to Thromboxane Receptor Mediated Cerebral Vasomotion and CBF Oscillations during Acute NO-Deficiency in Rats

), NO-deficiency is often associated with activation of thromboxane receptors (TP). In the present study we hypothesized that in the absence of NO, overactivation of the TP-receptor mediated cerebrovascular signaling pathway contributes to the development of vasomotion and CBF oscillations. synthesis by ozagrel (10 mg/kg iv.) attenuated it. In isolated MCAs U-46619 in a concentration of 100 nM, which induced weak and stable contraction under physiological conditions, evoked sustained vasomotion in the absence of NO, which effect could be completely reversed by inhibition of Rho-kinase by 10 µM Y-27632.These results suggest that hypersensitivity of the TP-receptor – Rho-kinase signaling pathway contributes to the development of low frequency cerebral vasomotion which may propagate to vasospasm in pathophysiological states associated with NO-deficiency

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Fast Cooling Phenomena with Ice Pellet Injection in the JIPP T-IIU Tokamak

"Ice pellet injection experiments were carried out in the JIPP T-IIU tokamak in order to study thermal (cooling) transport just after injection. The cut-off problem of ECE signals due to the rise in density has been resolved by careful measurements of temperature profile at a high time resolution (Delta t=2ms) during its decay phase. The phenomenon of ultra-fast cooling (so-called pre-cooling) has been identified using the two different methods of ECE and soft X-ray(SXR) measurements. In the outer region (r > r_inv) of the plasma the cooling propagation velocity is comparable to or slightly greater than the pellet velocity, while in the central region (r < r_inv) the propagation velocity is significantly greater than the pellet velocity. Ice pellet were injected into various kinds of JIPP T-IIU plasmas, the current and sawtooth phase of which had different values, including a no-sawtooth plasma. The existence of the sawtooth oscillation and arrival of a pellet near the inversion radius r_inv of the sawtooth oscillations have turned out to be necessary conditions for the pre-cooling, and even just after the sawtooth crash the pre-cooling may start around the q=1 surface, not at the plasma center . Simultaneous measurements of electron temperature and density profiles indicate that the central temperature always decreases before the central density increases. Some anomalous transport might be induced by pellet injection at the central region.