A comprehensive gene expression analysis at sequential stages of in vitro cardiac differentiation from isolated MESP1-expressing-mesoderm progenitors

In vitro cardiac differentiation of human pluripotent stem cells (hPSCs) closely recapitulates in vivo embryonic heart development, and therefore, provides an excellent model to study human cardiac development. We recently generated the dual cardiac fluorescent reporter MESP1mCherry/w NKX2-5eGFP/w line in human embryonic stem cells (hESCs), allowing the visualization of pre-cardiac MESP1+ mesoderm and their further commitment towards the cardiac lineage, marked by activation of the cardiac transcription factor NKX2-5. Here, we performed a comprehensive whole genome based transcriptome analysis of MESP1-mCherry derived cardiac-committed cells. In addition to previously described cardiac-inducing signalling pathways, we identified novel transcriptional and signalling networks indicated by transient activation and interactive network analysis. Furthermore, we found a highly dynamic regulation of extracellular matrix components, suggesting the importance to create a versatile niche, adjusting to various stages of cardiac differentiation. Finally, we identified cell surface markers for cardiac progenitors, such as the Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4), belonging to the same subfamily of LGR5, and LGR6, established tissue/cancer stem cells markers. We provide a comprehensive gene expression analysis of cardiac derivatives from pre-cardiac MESP1-progenitors that will contribute to a better understanding of the key regulators, pathways and markers involved in human cardiac differentiation and development

Off-line Roman Cursive Handwriting Recognition

This paper reviews the state of the art in off-line Roman cursive handwriting recognition. The input provided to an off-line handwriting recognition system is an image of a digit, a word, or- more generally- some text, and the system produces, as output, an ASCII transcription of the input. This task involves a number of processing steps, some of which are quite difficult. Typically, preprocessing, normalization, feature extraction, classification, and postprocessing operations are required. In this article we survey the state of the art, analyze recent trends, and try to identify challenges for future research in this field

Stem Cells Commitment on Graphene-Based Scaffolds

none8siIn the last years, a rapid development in production, and functionalization of graphene give rise to several products that have shown great potentials in many fields, such as nanoelectronics, energy technology, sensors, and catalysis. In this context we should not forget the biomedical application of graphene that became a new area with outstanding potential. The first study on graphene for biomedical applications has been performed by Dai in 2008 that reported the use of graphene oxide as an efficient nanocarrier for drug delivery. This pioneristic study opened the doors for the use of graphene in widespread biomedical applications such as drug/gene delivery, biological sensing and imaging, antibacterial materials, but also as biocompatible scaffold for cell culture and tissue engineering. The application of graphene-based scaffolds for tissue engineering applications is confirmed by the many exciting and intriguing literature reports over the last few years, that clearly confirm that graphene and its related substrates are excellent platforms for adhesion, proliferation, and differentiation of various cells such as human Mesenchymal stem cells, human neuronal stem cells, and induced pluripotent stem cells. Since most of the papers on this fields are related to in vitro studies, several future in vivo investigations need to be conducted in order to lead to its utilization as implantable tissue engineering material.mixedBuggio, Maurizio; Tatullo, Marco; Sivolella, Stefano; Gardin, Chiara; Ferroni, Letizia; Mijiritsky, Eitan; Piattelli, Adriano; Zavan, BarbaraBuggio, Maurizio; Tatullo, Marco; Sivolella, Stefano; Gardin, Chiara; Ferroni, Letizia; Mijiritsky, Eitan; Piattelli, Adriano; Zavan, Barbar