Decreasing erucic acid level by RNAi-mediated silencing of fatty acid elongase 1 (BnFAE1.1) in rapeseeds (Brassica napus L.)

The β-ketoacyl CoA synthase encoded by fatty acid elongase 1 gene (BnFAE1.1) is a rate-limiting enzyme regulating biosynthesis of erucic acid in rapeseeds (Brassica napus). To develop low level of erucic acid in rapeseeds by intron-spliced hairpin RNA, an inverted repeat unit of a partial BnFAE1.1 gene interrupted by a spliceable intron was cloned into pCAMBIA3301, and a seed-specific (Napin) promoter was used to control the transcription of the transgene. Four transgenic plants harboring a single copy of transgene were generated. Expression of endogenous BnFAE1.1 gene in developing T3 seeds was significantly reduced. In mature T3 seeds, erucic acid was decreased by 60.8 to 99.1% compared with wild type seeds, and accounted for 0.36 to 15.56% of total fatty acids. The level of eicosenoic acid was also greatly decreased. Furthermore, it resulted in a significant increase in the level of oleic acid, but total fatty acid content in T3 seeds was the same with that in wild type seeds. In conclusion, the expression of endogenous BnFAE1.1 was efficiently silenced by the designed RNAi silencer, causing a significant down-regulation in the level of erucic acid. Therefore, the RNAi-mediated post-transcriptional silencing of FAE1 gene to reduce oleic acid in rapeseeds was an efficient method to breed some new B. napus lines.Key words: Brassica napus L., fatty acid elongase, intron-spliced hairpin RNA, down-regulation, erucic acid

The Invisible Platform of E-Business: Trust

E-Business is the new business imperative in the age of Internet. With the features of Internet, business can extend their business activities across space and time. Without traditional fact-to-face interaction, most of the e-business activities can be done through networks. Since they do not have to see each other during e-business transaction, the levels of trust between members are pretty low. Mutual trust within a business, between suppliers and retailers, and between sellers and customers is the most important successful factors of e-business. Based on the review of literatures, we proposed that trust is the most important invisible platform for the e-business. In this article, we discuss the relationship between trust and three major components of e-business: (1) the trust for effective knowledge management (KM) within an organization; (2) the trust between organization and customer for effective customer relationship management (CRM); and (3) the trust between organizations for supply chain management (SCM)

An SU(4) Model of High-Temperature Superconductivity and Antiferromagnetism

We present an SU(4) model of high-temperature superconductivity having many similarities to dynamical symmetries known to play an important role in microscopic nuclear structure physics and in elementary particle physics. Analytical solutions in three dynamical symmetry limits of this model are found: an SO(4) limit associated with antiferromagnetic order; an SU(2) X SO(3) limit that may be interpreted as a d-wave pairing condensate; and an SO(5) limit that may be interpreted as a doorway state between the antiferromagnetic order and the superconducting order. The model suggests a phase diagram in qualitative agreement with that observed in the cuprate superconductors. The relationship between the present model and the SO(5) unification of superconductivity and antiferromagnetic order proposed by Zhang is discussed.Comment: A long paper extended from the early version cond-mat/9903150; accepted by Phys. Rev.

The 13N(d,n)14O Reaction and the Astrophysical 13N(p,g)14O Reaction Rate

$^{13}$N($p,\gamma$)$^{14}$O is one of the key reactions in the hot CNO cycle which occurs at stellar temperatures around $T_9$ $\geq$ 0.1. Up to now, some uncertainties still exist for the direct capture component in this reaction, thus an independent measurement is of importance. In present work, the angular distribution of the $^{13}$N($d,n$)$^{14}$O reaction at $E_{\rm{c.m.}}$ = 8.9 MeV has been measured in inverse kinematics, for the first time. Based on the distorted wave Born approximation (DWBA) analysis, the nuclear asymptotic normalization coefficient (ANC), $C^{^{14}O}_{1,1/2}$, for the ground state of $^{14}$O $\to$ $^{13}$N + $p$ is derived to be $5.42 \pm 0.48$ fm$^{-1/2}$. The $^{13}$N($p,\gamma$)$^{14}$O reaction is analyzed with the R-matrix approach, its astrophysical S-factors and reaction rates at energies of astrophysical relevance are then determined with the ANC. The implications of the present reaction rates on the evolution of novae are then discussed with the reaction network calculations.Comment: 17 pages and 8 figure

Activation of NRG1-ERBB4 signaling potentiates mesenchymal stem cell-mediated myocardial repairs following myocardial infarction

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