Ag-NOR karyotypes of five endemic pseudophoxinus bleeker, 1860 (Teleostei: Leuciscidae) species from anatolia

This study presents Ag-NOR (silver-nucleolus organizer region) karyotypes of five endemic Pseudophoxinus species from Anatolia. The specimens were collected from central and southern Anatolia. The collected specimens were carried alive to the laboratory. Chromosome slides were prepared and Ag-staining technique was applied. Ag-NOR metaphases were photographed with a camera-equipped light microscope. Karyotypes of this metaphases were arranged. Ag-NORs were distributed between the second and eighth submetacentric chromosome pairs in the studied species. This study reveals the details of the Ag-NOR distributions on the karyotypes and may improve the cytogenetic data of the genus. Published by Central Fisheries Research Institute (SUMAE) Trabzon, Turkey

Acanthobrama marmid ve Cyprinion macrostomus (Osteichthyes, Cyprinidae)'un konstitütif heterokromatini

Türkiye’de yaşayan iki Cyprinid Acanthobrama marmid ve Cyprinion macrostomus üzerinde konstitütif heterokromatini araştırıldı. Bu türlerin metafaz kromozomlarından C-band kalıpları rapor edildi. Bütün örneklerde diploid kromozom sayısı 2n=50 bulundu. Her iki türde de bütün kromozomların perisentromerik bölgelerinde Cband görüldü. Ayrıca A. marmid’de iki çift submetasentrik kromozomun kısa kollarında ve C. macrostomus’ta iki çift submeta-subtelosentrik kromozomun kısa kollarında C-band gözlendi. C-band blokları ve NOR bölgeleri arasında benzerlikler bulundu. Her iki türün C-band kalıpları arasında da benzerlikler mevcuttu.Constitutive heterochromatin were studied in two Cyprinids Acanthobrama marmid and Cyprinion macrostomus in Turkey. The C-band patterns of the metaphase chromosomes of these species were reported. Diploid chromosome number was 2n=50 in all specimens. The C-bands were seen in the pericentromeric regions of all chromosomes in both species. In addition C-bands were observed on the short arms of two pairs of submetacentric chromosomes of A. marmid and on the short arms of two pairs of submeta-subtelocentric chromosomes of C. macrostomus. There were similarities between C-band blocks and NOR regions. C-band patterns were also similar in both species

NOR Phenotype of Cyprinion macrostomus (Osteichthyes, Cyprinidae)

In this study, nucleolus organizer regions of Cyprinion macrostomus were investigated. The diploid number of chromosomes of this species was 2n=50 consisting of three pairs of metacentric, 12 pairs of submetacentric, six pairs of subtelocentric and four pairs of acrocentric chromosomes. The nucleolus organizer regions were detected at the end of the short arms of two pairs of medium sized submetacentric chromosomes. Nucleolus organizer region patterns were nearly identical with what that found in most other representatives of the Eurasian Cyprinids.In this study, nucleolus organizer regions of Cyprinion macrostomus were investigated. The diploid number of chromosomes of this species was 2n=50 consisting of three pairs of metacentric, 12 pairs of submetacentric, six pairs of subtelocentric and four pairs of acrocentric chromosomes. The nucleolus organizer regions were detected at the end of the short arms of two pairs of medium sized submetacentric chromosomes. Nucleolus organizer region patterns were nearly identical with what that found in most other representatives of the Eurasian Cyprinids

Fırat Nehri’ndeki Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae)’nin karyotip, NOR ve C-bantlama analizi

Fırat Nehir sisteminde yaşayan Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae)’nin karyotip, C-bantlama ve nükleolus organizatör bölge analizi yapıldı. Metafaz kromozomları böbrek hücrelerinden elde edildi. Diploid kromozom sayısı 19 çift meta-submetasentrik ve 6 çift subtelosentrik olmak üzere 2n = 50 ve kol sayısı 88 bulundu. Eşey kromozomu farklılaşması gözlenmedi. C-bantlama ile altı çift kromozomun perisentromerlerinde heterokromatin bölge tespit edildi. Orta büyüklükte 2 çift submeta-subtelosentrik kromozomun kısa kollarında NOR gözlendi. Bu çalışmanın, yapılacak olan sitogenetik araştırmalara katkı sağlayacağı düşünülmektedir.Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae) living in the Euphrates River system were analyzed in terms of their karyotype, C-banding, and nucleolus organizer region properties. Metaphase chromosome spreads were obtained from kidney cells. The diploid chromosome number was found to be 2n = 50, of which 19 pairs were meta-submetacentric and 6 pairs were subtelocentric, and the fundamental number was found to be 88. No sex chromosome differentiation was observed. C-banding revealed heterochromatin in the pericentromeric regions of 6 pairs of chromosomes. NORs were identified on the short arms of 2 pairs of medium-sized submeta-subtelocentric chromosomes. This study may contribute to other cytogenetic research