Micropropagation of Vaccinium bracteatum Thunb.

Vaccinium bracteatum Thunb. is a vigorous plant that is easy to grow, can adapt to various acid soil species and has good resistance to drought. It has fewer pest problems than most other blueberry cultivars, offering an advantage rootstock for various blueberry cultivars to resist pest and disease. However, it is relatively difficult to propagate and the cuttings can be difficult to root. The present study is an investigation into the effects of the types and combination of plant growth regulators on the tissue culture system of V. bracteatum Thunb. When nodal explants were cultured on media without plant growth regulators (PGRs), the shoots did not produce new buds, while all media supplemented with PGRs significantly affected adventitious bud proliferation in woody plant medium (WPM). Upon addition of PGRs, adventitious buds occurred and significant differences were observed (P < 0.05). The shoot length and proliferation coefficient in WPM basal medium containing 0 mg L-1 KT, 2.0 mg L-1 BA, 2.0 mg L-1 ZT and 2.0 mg L-1 TDZ was much more than other concentrations. The shoot length and proliferation reached 9.64 and 7.66 cm. For rooting, significant variation was observed (P < 0.05) between different concentrations of IBA and NAA. In general, the effect of IBA was much better than NAA. The root length gradually increased with increasing concentration of IBA from 0 to 2.0 mg L-1 followed by a decrease from 2.0 to 4.0 mg L-1. The highest root length and number was 3.16 and 3.33 cm respectively when the concentration of IBA was 2.0 mg L-1.Keywords: Vaccinium bracteatum Thunb., plant growth regulator, tissue culture, micropropagationAfrican Journal of Biotechnology Vol. 12(7), pp. 695-70

Sensitivity analysis of log interpretation parameters of through casing reservoir saturation logging

In the middle and late stages of oilfield production and development, the increase of production measures has resulted in a greater difference in reservoir physical properties between layers and a complex oil-water relationship. Most of the main reservoirs are highly flooded, the distribution of formation water salinity is complex, and the accuracy of remaining oil evaluation is relatively low. In order to solve the problem of inaccurate evaluation of remaining oil in high temperature, high pressure, low formation water salinity and low porosity reservoirs, it is necessary to improve the existing logging interpretation and evaluation technology of remaining oil saturation. It can provide a powerful basis for accurately finding oil layers with development potential, and for adjustment and judgment of potential layers in the middle and late stages of oilfield development. In this paper, the parameter sensitivity is calculated by normalizing the parameter change amplitude, which can better compare the sensitivity of water saturation interpretation of each parameter at different depths. It can also be obtained which factor has the greatest influence on the interpretation of water saturation at different depths. Calculating the partial derivative value corresponding to the capture cross-section value of shale and formation water can specifically compare the sensitivity of parameters to the results and find out the direction of parameter adjustment