Using Pretreatment of Carbon Monoxide Combined with Chlorine Dioxide and Lactic Acid to Maintain Quality of Vacuum-Packaged Fresh Beef

Due to microbial growth, beef easily gets corrupt in retail conditions, and the color and quality of the meat will be deteriorated. Therefore, hurdle technology, namely, pretreatment of carbon monoxide (CO), chlorine dioxide, and lactic acid, is used for vacuum-packaged beef to decontaminate beef and increase its quality stability. Beef was pretreated with 100% CO (C1), 100% CO and 50 mg/L chlorine dioxide (C2), and 100% CO and 50 mg/L chlorine dioxide and 30 g/L lactic acid (C3). The untreated samples were used as control (CK). During storage, the a⁎ color parameters of C1, C2, and C3 were significantly higher than that of CK, indicating CO pretreatment is a good way to maintain color appearance of beef, and chlorine dioxide and lactic acid did not affect the color-protecting role of CO on beef. C3 showed the strongest antimicrobial activity with the lowest total viable counts, followed by C2, C1, and CK. Samples in C3 also showed the lowest total volatile basic nitrogen, pH, thiobarbituric acid reactive substance, and metmyoglobin during the mid-late storage. Moreover, C3 can keep beef with higher unsaturated fatty acids. In conclusion, CO pretreatment combined with chlorine dioxide and lactic acid displayed efficient antimicrobial and color-stability activity for vacuum-packaged beef. It would be a potential way to use pretreatment of CO combined with chlorine dioxide and lactic acid to maintain the quality of vacuum-packaged beef

Thymoma and thymic carcinoma associated with multilocular thymic cyst: a clinicopathologic analysis of 18 cases

Abstract Background Multilocular thymic cysts (MTCs) associated with thymomas or thymic carcinomas (TCs) are rare and may be misdiagnosed as other benign cystic lesions. Methods We retrospectively analysed 18 cases of thymomas or TCs associated with MTCs, which were retrieved from 309 consecutive patients with thymomas or TCs in the Chinese population, emphasizing clinicopathologic characteristics, immunophenotypes and the prognostic impact. Results A total of 14 tumours were described as cystic or solid-cystic masses, and the other 4 tumours were described as solid masses. Histologically, 2 atypical type A, 2 type AB, 1 type B1, 8 type B2, 1 type B3, 1 microscopic thymoma (type A), 2 squamous cell carcinomas (SCCs) and 1 lymphoepithelioma-like carcinoma (LELC) were classified. Prominent multilocular cystic areas with chronic inflammation were observed. The follow-up ranged from 2 to 79 months. Sixteen patients survived without any evidence of recurrence after complete resection. Conclusions Our study suggests that thymomas or TCs associated with MTCs are rare in the Chinese population and have a better clinical behaviour than thymomas or TCs without MTCs. Our data also expand the histologic spectrum of thymomas or TCs accompanied by MTC. To our knowledge, this is the first report of atypical type A thymoma and LELC associated with MTCs

MOESM1 of Cyclin D3 predicts disease-free survival in breast cancer

Additional file 1: Table S1. Relationship between Cyclin D3 expression and clinicopathological features in breast cancer patients

Morphology of <i>ALK</i>-rearrangement lung adnenocarcinoma.

<p>(A) papillary and micropapillary patterns; (B) mucinous cribriform pattern consisting of abundant extracellular mucus and cribriform structures; (C) solid pattern with signet ring cells; (D) mucinous cribriform pattern often floating within mucus-filled alveolar spaces; (E) mucous cells in form of goblet cells; (F) solid pattern with hepatoid tumor cells having abundant eosinophilic cytoplasm, round nuclei, and prominent nucleoli; the tumor cell nuclei are relatively monomorphic.</p

Two patterns of <i>ALK</i> gene alteration detected by FISH using Vysis LSI ALK Dual Color breakpoint probe.

<p>(A) Two distinct red and green signals. (B) Isolated red signal.</p

Comparison between FISH and IHC.

<p>Abbreviation: FISH: Fluorescence In Situ Hybridization; IHC: immunohistochemistry.</p

Comparison between RT-PCR and IHC.

<p>Abbreviation: RT-PCR: Reverse Transcription-Polymerase Chain Reaction; IHC: immunohistochemistry.</p